Protein profiling of alpha-fetoprotein producing gastric adenocarcinoma

He, Liang; Ye, Fei; Liu, Qu; Wang, Daguang; Cui, Miao; Wei, Chengguo; Xing, Yanpeng; Lee, Peng; Suo, Jian; Zhang, David Y.

doi:10.18632/oncotarget.8571

Cited by 19 publications

(25 citation statements)

References 32 publications

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“…Moreover, elevated serum levels of AFP indicates the growth of malignant lung and bladder tumors as well as gastrointestinal cancers of stomach, pancreas, and colon (Mizejewski, 2014). High mortality and morbidity rates were reported to patients with AFP-positive-gastrointestinal cancer (AFP(+)GC) than AFP(-)GC-patients due to active cell proliferation, high mitotic rate, amplified cell invasion and migration, rapid tumor progression and advanced tumor stage (He et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

The Carcinogenic Agent Diethylnitrosamine Induces Early Oxidative Stress, Inflammation and Proliferation in Rat Liver, Stomach and Colon: Protective Effect of Ginger Extract

Mansour

Abdallah

Ibrahim

et al. 2019

Asian Pac J Cancer Prev

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Background:Diethylnitrosamine (DENA), a well-known dietary carcinogen, related to cancer initiation of various organs. The present study investigated the deleterious mechanisms involved in the early destructive changes of DENA in different organs namely, liver, stomach and colon and the potential protective effect of GE against these mechanisms. Methods:Adult male albino rats were assigned into four groups. A normal control group received the vehicle, another group was injected with a single necrogenic dose of DENA (200 mg/kg, i.p) on day 21. Two groups received oral GE (108 or 216 mg/kg) daily for 28 days. Sera, liver, stomach and colon were obtained 7 days after DENA injection. Serum aspartate transaminase and alanine transaminase were detected as well as reduced glutathione (GSH), malondialdehyde, nitric oxide metabolites, interleukin 1β, tumor necrosis factor (TNF-α), alpha-fetoprotein (AFP) and nuclear factor-erythroid 2-related factor2 (Nrf2) in liver, stomach and colon. Histopathological studies and immunohistochemical examination of cyclooxygenase-2 (COX2) were conducted.Results:DENA induced elevation in liver function enzymes with significant increase in oxidation and inflammation biomarkers and AFP while decreased levels of Nrf2 in liver, stomach and colon were detected. Histologically, DENA showed degenerative changes in hepatocytes and inflammatory foci. Inflammatory foci displayed increased expression of COX2 in immunohistochemical staining. GE-pretreatment improved liver function and restored normal GSH with significant mitigation of oxidative stress and inflammatory biomarkers compared to DENA-treated group. AFP was reduced by GE in both doses, while Nrf2 increased significantly. Histology and immunostaining of hepatic COX-2 were remarkably improved in GE-treated groups in a dose dependent manner. Conclusion:GE exerted a potential anti-proliferative activity against DENA in liver, stomach and colon via Nrf2 activation, whilst suppression of oxidation and inflammation.

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Section: Discussionmentioning

confidence: 99%

The Carcinogenic Agent Diethylnitrosamine Induces Early Oxidative Stress, Inflammation and Proliferation in Rat Liver, Stomach and Colon: Protective Effect of Ginger Extract

Mansour

Abdallah

Ibrahim

et al. 2019

Asian Pac J Cancer Prev

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“…Moreover, miR-122-5p was not correlated with liver metastasis of non-AFPGC in clinical settings (data not shown). He et al (30) revealed that upregulated Bcl-2 (anti-apoptotic protein) was correlated with liver metastasis of AFPGC in clinical settings. Similarly, the protein level of Bcl-2 was decreased by inhibition of miR-122-5p in the present study.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of apoptosis by miR‑122‑5p in α‑fetoprotein‑producing gastric cancer

et al. 2019

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α-Fetoprotein (AFP)-producing gastric cancer (AFPGC) is recognized as one of the most aggressive tumors with subsequent poor prognosis compared with common gastric cancer (GC) subtypes. However, the molecular mechanism remains to be elucidated. We previously identified that miR-122-5p could be a useful biomarker in AFPGC patients. We examined herein the biological function of miR-122-5p and the molecular mechanism underlying tumor progression in AFPGC. We used the AFPGC cell line (FU97) and miR-122-5p inhibitor to examine the function of miR-122-5p. Moreover, we investigated the possible targets of miR-122-5p. The expression level of miR-122-5p was significantly increased in the FU97 cell line than in common GC cell lines. Also, suppression of miR-122-5p significantly reduced AFP levels and proliferation in AFPGC through an induction of apoptosis. Western blotting revealed that the expression of anti-apoptotic protein (Bcl-2) was decreased and that of pro-apoptotic protein (caspase-3) was increased in miR-122-5p suppression of FU97. Moreover, we revealed that FOXO3 was an important target of miR-122-5p in AFPGC, which inhibited apoptosis and subsequently manifested aggressiveness. In conclusion, miR-122-5p inhibited apoptosis and facilitated tumor progression by targeting FOXO3 in AFPGC, which indicates the possibility of miR-122-5p as a potential therapeutic target in AFPGC.

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“…He et al, [38] using the protein pathway array, 11 of out of 286 proteins tested were found to be differentially expressed, and several of them correlated with prognosis of AFP producing gastric adenocarcinoma. Two of them were worth mentioning; XIAP (X-linked inhibitor of apoptosis) and IGFIrβ (Insulin-like growth factor-I receptor β).…”

Section: Molecular Biology Of Afp-gcmentioning

confidence: 99%

“…The 1-,3-,5-year survival rates for AFP>300ng/ml were 15.4%, 7.7%, and 0% respectively. In three studies [21,23,38,57], multivariate survival analysis revealed that AFP positivity to be the independent prognostic factor. In the study by Liang et.al.…”

Section: Prognosismentioning

confidence: 99%

“…In the study by Liang et.al. (38), in AFP producing gastric adenocarcinoma group, 27 patients had relapsed [84.4% (27/32)] compared with 28 in AFP nonproducing gastric adenocarcinoma group [62.2% (28/45)]. Mean relapse free survival (RFS) was 9 months (95%CI: 6.6-11.6 months) in the AFP producing gastric adenocarcinoma group, compared with 30 months (95% CI: 6.6-53.4 months) in the AFP non-producing gastric adenocarcinoma group.…”

Section: Prognosismentioning

confidence: 99%

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Literature Review of Gastric Cancer Producing Alphafetoprotein

Sh¹

2017

Gastrointest Cancer Res Ther

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Background: Gastric cancer is the third most common cause of cancerrelated death worldwide. Alpha-fetoprotein producing gastric cancer (AFP-GC) is a subtype of gastric cancer which has more aggressive behavior than the common type. Awareness and understanding of this disease entity are of paramount importance, because treatment is different from that of the classical gastric cancer. Method: The literature search started from 1970, when the first patient of AFP-GC was reported, until March of 2017, using Google search engine, PUB Med to identify relevant articles on alpha-fetoprotein producing gastric cancer. Subsequent references searches were done from the retrieved articles. Results: Since the first case of alpha-fetoprotein producing gastric cancer was first reported, many cases have been reported, mostly in Asia. Although AFP-GCs have been recognized in Japan and parts of Asia for more than 40 years, there were only a few publications about AFP-GC outside of Asia. In this article, I review literature concerning the clinicopathological features, immunoreactivity, molecular biology, treatment, and prognosis of AFP-GC. Conclusion: In the literature, some potential protein markers were identified, and cellular pathways involved in AFP-GCs were suggested. Two types of targeted drugs have been used to treat such patients with a good response. Further studies of the roles of these markers and pathways may shed light on developing more effective drugs to treat patients with AFP-GC.

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Protein profiling of alpha-fetoprotein producing gastric adenocarcinoma

Cited by 19 publications

References 32 publications

The Carcinogenic Agent Diethylnitrosamine Induces Early Oxidative Stress, Inflammation and Proliferation in Rat Liver, Stomach and Colon: Protective Effect of Ginger Extract

The Carcinogenic Agent Diethylnitrosamine Induces Early Oxidative Stress, Inflammation and Proliferation in Rat Liver, Stomach and Colon: Protective Effect of Ginger Extract

Inhibition of apoptosis by miR‑122‑5p in α‑fetoprotein‑producing gastric cancer

Literature Review of Gastric Cancer Producing Alphafetoprotein

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