Protein profiling of zebrafish embryos unmasks regulatory layers during early embryogenesis

da Silva Pescador, Gabriel; Baia Amaral, Danielson; Varberg, Joseph M.; Zhang, Ying; Hao, Yan; Florens, Laurence; Bazzini, Ariel A.

doi:10.1016/j.celrep.2024.114769

Cell Reports

2024

DOI: 10.1016/j.celrep.2024.114769

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Protein profiling of zebrafish embryos unmasks regulatory layers during early embryogenesis

Gabriel da Silva Pescador,

Danielson Baia Amaral,

Joseph M. Varberg

et al.

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Critical role of Spatio-Temporally Regulated Maternal RNAs in Zebrafish Embryogenesis

Kushawah,

Amaral,

Hassan

et al. 2024

Preprint

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The maternal-to-zygotic transition shifts regulatory control from maternal to zygotic messenger RNAs (mRNA) through maternal mRNA degradation. While temporal aspects of maternal mRNA decay are known, spatial mechanisms remain underexplored. Using CRISPR-Cas9 and CRISPR-Cas13d systems, we functionally dissected the contribution of maternal versus zygotic fractions and overcame challenges of studying embryonic lethal genes. We identified differentially distributed maternal mRNAs in specific cells and evidenced the critical role of five maternal mRNAs, cth1, arl4d, abi1b, foxa and lhx1a, in embryogenesis. Further, we focused on the functionally uncharacterized cth1 gene, revealing its essential role in gametogenesis and embryogenesis. Cth1 acts as a spatio-temporal RNA decay factor regulating mRNA stability and accumulation of its targets in a spatio-temporal manner through 3'UTR recognition during early development. Furthermore, Cth1 3'UTR drives its spatio-temporal RNA localization. Our findings provide new insights into spatio-temporal RNA decay mechanisms and highlight dual CRISPR-Cas strategies in studying embryonic development.

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Critical role of Spatio-Temporally Regulated Maternal RNAs in Zebrafish Embryogenesis

Kushawah,

Amaral,

Hassan

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

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Protein profiling of zebrafish embryos unmasks regulatory layers during early embryogenesis

Cited by 1 publication

References 82 publications

Critical role of Spatio-Temporally Regulated Maternal RNAs in Zebrafish Embryogenesis

Critical role of Spatio-Temporally Regulated Maternal RNAs in Zebrafish Embryogenesis

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