Protein–Protein and Protein–Membrane Associations in the Lignin Pathway

Bassard, Jean‐Etienne; Richert, Ludovic; Geerinck, Jan; Renault, H; Duval, Frédéric; Ullmann, Pascaline; Schmitt, Martine; Meyer, Etienne H.; Mutterer, Jérôme; Boerjan, Wout; Jaeger, Geert De; Mély, Yves; Goossens, Alain; Werck‐Reichhart, Danièle

doi:10.1105/tpc.112.102566

Cited by 133 publications

(128 citation statements)

References 80 publications

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“…These findings are consistent with the hypothesis that the differential expression of the four 4CLs leads to their different biological functions (Costa et al, 2005;Soltani et al, 2006;Bassard et al, 2012). To examine the spatiotemporal patterns of expression of the four 4CLs, we generated transgenic Arabidopsis plants carrying 4CL promoter-GUS constructs and analyzed plants throughout development.…”

Section: Promoter-gus Analysis Of Four 4cls In Arabidopsissupporting

confidence: 82%

Four isoforms of Arabidopsis thaliana 4-coumarate: CoA ligase (4CL) have overlapping yet distinct roles in phenylpropanoid metabolism

et al. 2015

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The biosynthesis of lignin, flavonoids, and hydroxycinnamoyl esters share the first three enzymatic steps of the phenylpropanoid pathway. The last shared step is catalyzed by 4-coumarate:CoA ligase (4CL), which generates p-coumaroyl CoA and caffeoyl CoA from their respective acids. Four isoforms of 4CL have been identified in Arabidopsis (Arabidopsis thaliana). Phylogenetic analysis reveals that 4CL1, 4CL2, and 4CL4 are more closely related to each other than to 4CL3, suggesting that the two groups may serve different biological functions. Promoter-GUS analysis shows that 4CL1 and 4CL2 are expressed in lignifying cells. In contrast, 4CL3 is expressed in a broad range of cell types, and 4CL3 has acquired a distinct role in flavonoid metabolism. Sinapoylmalate, the major hydroxycinnamoyl ester found in Arabidopsis, is greatly reduced in the 4cl1 4cl3 mutant, showing that 4CL1 and 4CL3 function redundantly in its biosynthesis. 4CL1 accounts for the majority of the total 4CL activity, and loss of 4CL1 leads to reduction in lignin content but no growth defect. The 4cl1 4cl2 and 4cl1 4cl2 4cl3 mutants are both dwarf but do not have further reduced lignin than the 4cl1 mutant, indicating that either 4CL1 or 4CL2 is required for normal plant growth. Although 4CL4 has a limited expression profile, it does make a modest contribution to lignin biosynthesis. Together, these data show that the four isoforms of 4CL in Arabidopsis have overlapping yet distinct roles in phenylpropanoid metabolism.

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Section: Promoter-gus Analysis Of Four 4cls In Arabidopsissupporting

confidence: 82%

Four isoforms of Arabidopsis thaliana 4-coumarate: CoA ligase (4CL) have overlapping yet distinct roles in phenylpropanoid metabolism

et al. 2015

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“…C3H/C4H? : Interaction between C3H and C4H has been shown for both Arabidopsis and poplar, but activity of the C3H/ C4H heterodimer for the conversion of p-coumaric acid to caffeic acid has only been shown for poplar (Bassard et al, 2012;Chen et al, 2011). of lignin deposition in the xylem of ccr1 ProSNBE:CCR1 appeared to be sufficient for the complete recovery of the stem biomass yield of ccr1 ProSNBE:CCR1 lines.…”

Section: Discussion Vessels Act As Good Neighbors To Xylary Fibersmentioning

confidence: 99%

Vessel-Specific Reintroduction of CINNAMOYL-COA REDUCTASE1 (CCR1) in Dwarfed ccr1 Mutants Restores Vessel and Xylary Fiber Integrity and Increases Biomass

et al. 2017

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Lignocellulosic biomass is recalcitrant toward deconstruction into simple sugars due to the presence of lignin. To render lignocellulosic biomass a suitable feedstock for the bio-based economy, plants can be engineered to have decreased amounts of lignin. However, engineered plants with the lowest amounts of lignin exhibit collapsed vessels and yield penalties. Previous efforts were not able to fully overcome this phenotype without settling in sugar yield upon saccharification. Here, we reintroduced CINNAMOYL-COENZYME A REDUCTASE1 (CCR1) expression specifically in the protoxylem and metaxylem vessel cells of Arabidopsis (Arabidopsis thaliana) ccr1 mutants. The resulting ccr1 ProSNBE:CCR1 lines had overcome the vascular collapse and had a total stem biomass yield that was increased up to 59% as compared with the wild type. Raman analysis showed that monolignols synthesized in the vessels also contribute to the lignification of neighboring xylary fibers. The cell wall composition and metabolome of ccr1 ProSNBE:CCR1 still exhibited many similarities to those of ccr1 mutants, regardless of their yield increase. In contrast to a recent report, the yield penalty of ccr1 mutants was not caused by ferulic acid accumulation but was (largely) the consequence of collapsed vessels. Finally, ccr1 ProSNBE:CCR1 plants had a 4-fold increase in total sugar yield when compared with wild-type plants.

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“…A compendium of protein-protein interactions in Arabidopsis was constructed combining the following sources: BioGRID 3.2.110 (ChatrAryamontri et al, 2013), CORNET (only experimentally validated interactions) (De Bodt et al, 2012), STRINGv9.1 (only category Binding) (Franceschini et al, 2013), EVEX (only category binding) (Van Landeghem et al, 2013), and a TAP data set assembled from literature (Takahashi et al, 2008;Pauwels et al, 2010;Van Leene et al, 2010;Bassard et al, 2012;Domenichini et al, 2012;Eloy et al, 2012;Antoni et al, 2013;Cromer et al, 2013;Di Rubbo et al, 2013;Heijde et al, 2013;Spinner et al, 2013;Fonseca et al, 2014;Gadeyne et al, 2014;Perez et al, 2014;Vercruyssen et al, 2014). After removing redundancy and self-interactions, this lead to a set with a total of 46,113 interactions between 9813 proteins.…”

Section: Ppi Data In Arabidopsismentioning

confidence: 99%