Protein/Protein, DNA/DNA and DNA/Protein based vaccination strategies using truncated Omp2b against Brucella infection in BALB/c Mice

Golshani, Maryam; Rafati, Sima; Nejati-Moheimani, Mehdi; Pourabdi, Somaye; Arsang, Amin; Bouzari, Saeid

doi:10.1016/j.ijmm.2017.03.004

Cited by 9 publications

(8 citation statements)

References 38 publications

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“…Cytokines play a crucial role in differentiation of naive CD4 + T cells [57]. Figure 5 shows that aP-GSe enhanced production of not only IFN-γ, IL-2, and IL-12 but also IL-4, IL-6, and IL10, suggesting that both Th1 and Th2 subset cells were activated [58][59][60][61]. Cytokine-cytokine receptor interaction on naive CD4 + T cells activates the Janus kinase and signal transducers and activators of transcription (JAK-STAT) pathways, JAK1/2 and STAT1/3/4 induced by IFN-γ and IL-12 to stimulate T-bet and further IFN-γ production, and IL-4 triggers JAK1/3 and STAT6 to activate transcription factors GATA-3 [57].…”

Section: Discussionmentioning

confidence: 99%

A Solution with Ginseng Saponins and Selenium as Vaccine Diluent to Increase Th1/Th2 Immune Responses in Mice

Wang

Cui

et al. 2020

Journal of Immunology Research

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Pseudorabies is an important infectious disease of swine, and immunization using attenuated pseudorabies virus (aPrV) vaccine is a routine practice to control this disease in swine herds. This study was to evaluate a saline solution containing ginseng stem-leaf saponins (GSLS) and sodium selenite (Se) as a vaccine adjuvant for its enhancement of immune response to aPrV vaccine. The results showed that aPrV vaccine diluted with saline containing GSLS-Se (aP-GSe) induced significantly higher immune responses than that of the vaccine diluted with saline alone (aP-S). The aP-GSe promoted higher production of gB-specific IgG, IgG1, and IgG2a, neutralizing antibody titers, secretion of Th1-type (IFN-γ, IL-2, IL-12), and Th2-type (IL-4, IL-6, IL-10) cytokines, and upregulated the T-bet/GATA-3 mRNA expression when compared to aP-S. In addition, cytolytic activity of NK cells, lymphocyte proliferation, and CD4 + /CD8 + ratio was also significantly increased by aP-GSe. More importantly, aP-GSe conferred a much higher resistance of mice to a field virulent pseudorabies virus (fPrV) challenge. As the present study was conducted in mice, further study is required to evaluate the aP-GSe to improve the vaccination against PrV in swine.

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Section: Discussionmentioning

confidence: 99%

A Solution with Ginseng Saponins and Selenium as Vaccine Diluent to Increase Th1/Th2 Immune Responses in Mice

Wang

Cui

et al. 2020

Journal of Immunology Research

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“…[ 8 ] We have previously analyzed the immunogenicity and protective efficacy of B. abortus 544 Omp2b and L7/L12 proteins in BALB/c mice. [ 19 22 23 ] Our successful results encouraged us to design a chimera based on SOmp2b and L7/L12 immunogens.…”

Section: Discussionmentioning

confidence: 99%

“…[ 8 10 ] We have previously cloned and expressed the SOmp2b (a form lacking the signal peptide) protein and also the truncated form (aa163-aa362), successfully. [ 21 22 ] Our in silico analysis of Om2b protein indicated that the antigen is potential to induce both B- and T-cell mediated immune responses and it can be evaluated as a new subunit vaccine candidate against brucellosis. Moreover, further in vivo evaluation of the immunogenicity and protective efficacy of the SOmp2b and its truncated form (TOmp2b) done by us showed that SOmp2b and TOmp2b are potential vaccine candidates against B. melitensis and B. abortus infections.…”

Section: Introductionmentioning

confidence: 99%

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In silico Design, and In vitro Expression of a Fusion Protein Encoding Brucella abortus L7/L12 and SOmp2b Antigens

et al. 2018

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Background:L7/L12 is a protective antigen conserved in main Brucella pathogens and is considered as potential vaccine candidate. Outer membrane protein 2b is an immunogen conserved in all Brucella pathogens.Materials and Methods:The purpose of the current study was to in silico design a L7/L12-SOmp2b fusion protein and in vitro production of the chimera. Two possible fusion forms, L7/L12-SOmp2b and SOmp2b-L7/L12, were subjected to in silico modeling and analysis. Cloning and expression of the fusion protein has been done in the pET28a vector and Escherichia coli Bl21 (DE3), respectively.Results:Analysis and validation of the fusion proteins three-dimensional models showed that both models are in the range of native proteins. However, L7/L12-SOmp2b structure was more valid than the SOmp2b-L7/L12 model and subjected to in vitro production. The major histocompatibility complex II (MHC-II) epitope mapping using Immune Epitope DataBase indicated that the model contained good MHC-II binders. The L7/L12-Omp2b coding sequence was cloned in pET28a vector. The fusion was successfully expressed in E. coli BL21 by induction with isopropyl-β-d-thiogalactopyranoside. The rL7/L12-SOmp2b was purified with Ni-NTA column. The yield of the purified rL7/L12-SOmp2b was estimated by Bradford method to be 240 μg/ml of the culture. Western blot analysis revealed a specific reactivity with purified rL7/L12-SOmp2b produced in E. coli cells and showed the expression in the prokaryotic system.Conclusions:Our data indicates that L7/L12-SOmp2b fusion protein has a potential to induce both B- and T-cell-mediated immune responses and it can be evaluated as a new subunit vaccine candidate against brucellosis.

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“…For instance, in BALB/c mice, low levels of IgG2a with high levels in IgG1 indicate a Th2 response, while high levels of IgG2a (despite an increase in IgG1) is strongly linked to Th1 responses. [32][33][34][35] Vaccine delivery with MN arrays has induced multiple antigen-specific antibodies, including IgG1, [11] IgG2a (for BALB/c mice), [15,35] IgG2c (for C57BL/6 mice), [11] IgA, [36] and IgE [37] antibodies, but the effect of MN design on antibody dominance is poorly investigated.…”

Section: Introductionmentioning

confidence: 99%

An Ultrahigh‐Density Microneedle Array for Skin Vaccination: Inducing Epidermal Cell Death by Increasing Microneedle Density Enhances Total IgG and IgG1 Immune Responses

Coffey

Burg

Rananakomol

et al. 2022

Advanced NanoBiomed Research

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Many types of microneedle (MN) arrays have been tested for delivery of vaccines to the skin. However, the effect of MN geometry/array design on antibody production is still unclear. Reports suggest that systemic immune responses may be affected by how MN arrays “mechanically” deliver vaccines, which can induce cell damage and act as an adjuvant. This includes parameters such as MN length/insertion depth, delivery energy/velocity, MN shape, and density. However, these effects have not been systematically investigated. Herein, the effect of MN density on antibody responses to influenza vaccination is assessed, keeping all other variables constant. MN arrays are manufactured within the “high‐density range” from 5 k microneedles cm−2 (n cm−2) to the “ultrahigh” 30 k n cm−2. Prior to this study, the highest MN density used for vaccination is 20 k n cm−2. Thus, MN array vaccination is evaluated over an unprecedented range. Cell viability is assessed in the skin after application and antibody responses at days 21/63. It is demonstrated that increasing MN density from 5 to 30 k n cm−2 increases both epidermal cell death and anti‐influenza IgG1, without an increase in anti‐influenza IgG2a. This suggests that MN density has a direct adjuvant effect on immune responses through Th2‐mediated signalling—a response critical for human vaccination.

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Protein/Protein, DNA/DNA and DNA/Protein based vaccination strategies using truncated Omp2b against Brucella infection in BALB/c Mice

Cited by 9 publications

References 38 publications

A Solution with Ginseng Saponins and Selenium as Vaccine Diluent to Increase Th1/Th2 Immune Responses in Mice

A Solution with Ginseng Saponins and Selenium as Vaccine Diluent to Increase Th1/Th2 Immune Responses in Mice

In silico Design, and In vitro Expression of a Fusion Protein Encoding Brucella abortus L7/L12 and SOmp2b Antigens

An Ultrahigh‐Density Microneedle Array for Skin Vaccination: Inducing Epidermal Cell Death by Increasing Microneedle Density Enhances Total IgG and IgG1 Immune Responses

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