Protein-protein interaction between ezrin and p65 in human breast cancer cells

Tang, Ruixiang; Li, F X; Shao, Wen-Feng; Yu, Xiaomei; Xiong, Jun

doi:10.4238/gmr.15028334

Cited by 6 publications

(4 citation statements)

References 29 publications

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“…Several studies have demonstrated that knocking down ezrin and P65 expression induces tumor metastasis in different cancers [61, 62]. Furthermore, Tang et al [63] demonstrated that ezrin and P65 were physically associated with one another. We hypothesize that the interaction between ezrin and P65 is associated with the activation of the NF-κB pathway leading to breast cancer metastasis.…”

Section: Discussionmentioning

confidence: 99%

Identification and analysis of long non-coding RNA related miRNA sponge regulatory network in bladder urothelial carcinoma

Wang

Zhang

et al. 2019

Cancer Cell Int

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Background: The aim of this study was to investigate the regulatory network of lncRNAs as competing endogenous RNAs (ceRNA) in bladder urothelial carcinoma (BUC) based on gene expression data derived from The Cancer Genome Atlas (TCGA). Materials and methods: RNA sequence profiles and clinical information from 414 BUC tissues and 19 non-tumor adjacent tissues were downloaded from TCGA. Differentially expressed RNAs derived from BUC and non-tumor adjacent samples were identified using the R package "edgeR". Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was performed using the "clusterProfiler" package. Gene ontology and protein-protein interaction (PPI) networks were analyzed for the differentially expressed mRNAs using the "STRING" database. The network for the dysregulated lncRNA associated ceRNAs was then constructed for BUC using miRcode, miRTarBase, miRDB, and Tar-getScan. Cox regression analysis was performed to identify independent prognostic RNAs associated with BUC overall survival (OS). Survival analysis for the independent prognostic RNAs within the ceRNA network was calculated using Kaplan-Meier curves. Results: Based on our analysis, a total of 666, 1819 and 157 differentially expressed lncRNAs, mRNAs and miRNAs were identified respectively. The ceRNA network was then constructed and contained 59 lncRNAs, 23 DEmiRNAs, and 52 DEmRNAs. In total, 5 lncRNAs (HCG22, ADAMTS9-AS1, ADAMTS9-AS2, AC078778.1, and AC112721.1), 2 miRNAs (hsa-mir-145 and hsa-mir-141) and 6 mRNAs (ZEB1, TMEM100, MAP1B, DUSP2, JUN, and AIFM3) were found to be related to OS. Two lncRNAs (ADAMTS9-AS1 and ADAMTS9-AS2) and 4 mRNA (DUSP2, JUN, MAP1B, and TMEM100) were validated using GEPIA. Thirty key hub genes were identified using the ranking method of degree. KEGG analysis demonstrated that the majority of the DEmRNAs were involved in pathways associated with cancer. Conclusion: Our findings provide an understanding of the important role of lncRNA-related ceRNAs in BUC. Additional experimental and clinical validations are required to support our findings.

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Section: Discussionmentioning

confidence: 99%

Identification and analysis of long non-coding RNA related miRNA sponge regulatory network in bladder urothelial carcinoma

Wang

Zhang

et al. 2019

Cancer Cell Int

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“…12 Blood vessels enable the exchange of nutrient and catabolites for cancer cells and allow communication between primary and metastatic tumours. 13,14 Therefore, angiogenesis, the process of new blood vessel growth, is crucial for cancer development and is a potential target for cancer therapy.…”

Section: Introductionmentioning

confidence: 99%

Ezrin promotes breast cancer progression by modulating AKT signals

Kong

Lin

et al. 2019

Br J Cancer

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BACKGROUND: Ezrin, which is known as a cytoskeleton linker protein, is closely linked with the metastatic progression of cancer and is frequently abnormally expressed in aggressive cancer types. However, the possible involvement of Ezrin in metastasis and angiogenesis in breast cancer remains unclear. METHODS: Immunohistochemical analysis of Ezrin was performed on both BC samples (n = 117) and normal epithelium samples (n = 47). In vivo and in vitro assays were performed to validate the effect of Ezrin on AKT pathway-mediated BC progression. RESULTS: In this study, Ezrin was found to be upregulated in BC tissues, which was linked with aggressive tumour characteristics and poor prognosis. Moreover, we showed that Ezrin promotes BC proliferation, migration, invasion, and angiogenesis in vitro and in vivo. Mechanistic analysis showed that Ezrin interacted with AKT, and promoted its kinase activity, thereby regulating the AKT pathway in BC. CONCLUSIONS: In all, we propose a model for an Ezrin/AKT oncoprotein axis, which provides novel insight into how Ezrin contributes to BC progression.

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“…MicroRNA-183 can inhibit metastasis of cancer cells by inhibiting ezrin protein in lung (15) and breast cancer (16). In addition, it can also inhibit migration of lung cancer cells by downregulating WISP2 protein or upregulating the expression of S100P protein (17).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑183 and microRNA‑141 are potential risk factors for poor prognosis in patients with nasopharyngeal carcinoma

Lian

2018

Oncol Lett

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This study investigated whether microRNA-183 and microRNA-141 in nasopharyngeal carcinoma (NPC) lesions are potential risk factors for poor prognosis. A total of 317 NPC patients admitted to Zhengzhou Central Hospital Affiliated to Zhengzhou University from January 2010 to March 2015 were included. Reverse transcription-quantitative PCR (RT-qPCR) was used to detect the expression of microRNA-183 and microRNA-141 in lesions and adjacent tissues, and the relationship between the microRNA-183 and microRNA-141 expression levels and prognosis was analyzed. The expression levels of microRNA-183 and microRNA-141 in lesions were significantly higher than those in adjacent tissues (p<0.05). Patients with distant metastasis had significantly higher expression levels of microRNA-183 and microRNA-141 than patients without distant metastasis (p<0.01). Patients with disease-free survival (DFS) <3 years showed significantly higher expression levels of microRNA-183 and microRNA-141 than those with DFS ≥3 years (p<0.01). NPC patients with high expression levels of microRNA-183 and microRNA-141 showed poor prognosis. MicroRNA-183 and microRNA-141 may play an important role in the distant metastasis of NPC, and have a great impact on prognosis.

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Protein-protein interaction between ezrin and p65 in human breast cancer cells

Cited by 6 publications

References 29 publications

Identification and analysis of long non-coding RNA related miRNA sponge regulatory network in bladder urothelial carcinoma

Identification and analysis of long non-coding RNA related miRNA sponge regulatory network in bladder urothelial carcinoma

Ezrin promotes breast cancer progression by modulating AKT signals

MicroRNA‑183 and microRNA‑141 are potential risk factors for poor prognosis in patients with nasopharyngeal carcinoma

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