Protein-protein interaction network and mechanism analysis in ischemic stroke

Quan, Z.; Yuan, Quan; Wei, Bo-Bo; Fang, De-ning; Yu, Weidong; Jia, Hao; Quan, Weili; Liu, Yuguang; Wang, Qihong

doi:10.3892/mmr.2014.2696

Cited by 17 publications

(16 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many important biological processes were revealed in our study, such as cell death, programmed cell death, MAPK signaling pathway, which are consistent with the findings of previous studies 14 . There were 5 phosphate-related processes in the unique biological processes of module 8, accounting for 17.24%.…”

Section: Discussionsupporting

confidence: 93%

Crosstalk between miRNAs and their regulated genes network in stroke

Yuan

Kang

et al. 2016

Sci Rep

View full text Add to dashboard Cite

In recent years, more and more studies focus on the roles of genes or miRNAs in stroke. However, the molecular mechanism connecting miRNAs and their targetgenes remains unclear. The aim of this study was to determine the differential regulation and correlations between miRNAs and their targetgenes in human stroke. Stroke-related miRNAs were obtained from the Human MicroRNA Disease Database (HMDD) and their targetgenes were generated from three independent sources. Kappa score was used to create the network and the functional modules. A total of 11 stroke-related miRNAs were identified from the HMDD and 441 overlapping targetgenes were extracted from the three databases. By network construction and GO analysis, 13 functional modules, 186 biological processes, and 21 pathways were found in the network, of which functional module 8 was the largest module, cellular-related process and phosphate-related process were the most important biological processes, and MAPK signaling pathway was the most significant pathway. In our study, all miRNAs regulate the stroke modular network by their targetgenes. After the validation of miRNAs, we found that miR-605 and miR-181d were highly expressed in the blood of stroke patients which never reported before may supply novel target for treatment.

show abstract

Section: Discussionsupporting

confidence: 93%

Crosstalk between miRNAs and their regulated genes network in stroke

Yuan

Kang

et al. 2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Numerous murine ischemic stroke studies have noted that CXCL10 is significantly upregulated within the ischemic region after both transient and permanent middle cerebral artery occlusion . In one of these studies, inhibition of IFN‐ γ signaling prior to middle cerebral artery occlusion blocked induction of CXCL10 and reduced infarct volume, T‐cell infiltration, and neurodegeneration .…”

Section: Discussionmentioning

confidence: 99%

CCL2 and CXCL10 are associated with poor outcome after intracerebral hemorrhage

Landreneau

Mullen

Messé

et al. 2018

Ann Clin Transl Neurol

View full text Add to dashboard Cite

ObjectiveIntracerebral hemorrhage carries a high mortality and survivors are frequently left with significant disability. Immunological mechanisms may play an important role in hemorrhage‐induced brain injury, however, research linking these mechanisms with clinical outcome remains limited. We aim to identify serum inflammatory mediators that are associated with outcome after intracerebral hemorrhage in order to translate data from experimental models to a patient cohort and identify potential targets worthy of reverse translation.MethodsA prospective cohort study at two comprehensive stroke centers enrolled patients with spontaneous intracerebral hemorrhage. Peripheral blood was collected at 6, 24, and 72 h from onset. Functional outcome was assessed at 90 days using the modified Rankin Scale (mRS). Serum inflammatory mediators were measured using multiplex ELISA. Multivariable modeling identified serum biomarkers independently associated with functional outcome at 90 days.Results115 patients completed the study. At 6 h after onset, patients with elevated CCL2 had worse mRS score at day 90 (OR 4.07, 95% CI 1.27–13.10, P = 0.02) after adjusting for age, gender, ICH volume, IVH, infratentorial location and NIHSS score. At 24 and 72 h after onset, elevation in CXCL10 was independently associated with worse 90 days mRS score (24 h: OR 8.08, 95% CI 2.69–24.30, P < 0.001; 72 h: OR 3.89, 95% CI 1.12–13.49, P = 0.03).InterpretationAcute and subacute elevations in specific immune factors are associated with poor outcome, highlighting potential pathways that may contribute to ongoing brain injury in patients with intracerebral hemorrhage.

show abstract

“…Hematopoietic cell lineage and Cytokine-cytokine receptor interaction were also found in middle cerebral artery occlusion induced ischemic stroke mice (Quan et al 2015). Furthermore, a recent study showed affected Ribosome, Toll-like receptor signaling pathway, MAPK signaling pathway, and Chemokine signaling pathway in the ischemic rodent brain (Liang et al 2015).…”

Section: Discussionmentioning

confidence: 96%

“…And it's produced by many different cells including monocytes, macrophages, fibroblasts, endothelial cells, keratinocytes, mast cells, T-lymphocytes, and also by microglia and astrocytes (Ferrarese et al 1999). An increasing number of experimental observations showed that IL6 plays a central role in the pathogenesis of several ischemic cardiovascular disorders, including ischemic stroke (Quan et al 2015). A recent report suggested that IL6…”

Section: Discussionmentioning

confidence: 99%

Peer Review #1 of "Integrated analysis of ischemic stroke datasets revealed sex and age difference in anti-stroke targets (v0.2)"

2016

View full text Add to dashboard Cite

Ischemic stroke is a common neurological disorder and the burden in the world is growing. This study aims to explore the effect of sex and age difference on ischemic stroke using an integrated microarray datasets. The results showed a dramatic difference in whole gene expression profiles and influenced pathways between male and female, and also in the old and young individuals. Furthermore, compared with old male, old female patients showed more serious biological function damage. However, female showed less affected pathways than male in young subjects. Functional interaction networks showed these differential expression genes were mostly related to immune and inflammation-related functions. In addition, we found ARG1 and MMP9 were up-regulated in total and all subgroups. Importantly, IL1A, ILAB, IL6 and TNF and other anti-stroke target genes were up-regulated in males. However, these anti-stroke target genes showed low expression in females. This study found huge sex and age differences in ischemic stroke especially the opposite expression of anti-stroke target genes. Future studies are needed to uncover these pathological mechanisms, and to take appropriate pre-prevention, treatment and rehabilitation measures.

show abstract

Protein-protein interaction network and mechanism analysis in ischemic stroke

Cited by 17 publications

References 40 publications

Crosstalk between miRNAs and their regulated genes network in stroke

Crosstalk between miRNAs and their regulated genes network in stroke

CCL2 and CXCL10 are associated with poor outcome after intracerebral hemorrhage

Peer Review #1 of "Integrated analysis of ischemic stroke datasets revealed sex and age difference in anti-stroke targets (v0.2)"

Contact Info

Product

Resources

About