Protein–Protein Interactions in Plant Thioredoxin Dependent Systems

Meyer, Yves; Miginiac‐Maslow, Myroslawa; Schürmann, Peter; Jacquot, Jean‐Pierre

doi:10.1002/9781119312994.apr0056

Cited by 6 publications

(3 citation statements)

References 99 publications

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“…The catalytic properties of the target enzymes are modified in a manner that depends on their function in the organism's metabolism. Different aspects of this regulatory system have been treated in a number of recent reviews (Jacquot et al, 1997a;Meyer et al, 1999;Ruelland and Miginiac-Maslow, 1999;Dai et al, 2000a;Follmann, 2000;Schürmann and Jacquot, 2000;Meyer et al, 2001;Schürmann and Buchanan, 2001;Schürmann, 2003a,b;Dai et al, 2004Dai et al, , 2005Walters and Johnson, 2004). Here we will concentrate on FTR and its interactions with Fd and thioredoxin.…”

Section: Ferredoxin:thioredoxin Reductasementioning

confidence: 96%

The Interaction of Ferredoxin with Ferredoxin-Dependent Enzymes

Hase

Schürmann

Knaff

Advances in Photosynthesis and Respiration

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Section: Ferredoxin:thioredoxin Reductasementioning

confidence: 96%

The Interaction of Ferredoxin with Ferredoxin-Dependent Enzymes

Hase

Schürmann

Knaff

Advances in Photosynthesis and Respiration

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“…The proteomics has an important characteristic when compared with the genomics, that is, proteins have a direct influence on each other [31]. The realization of the function of a protein usually depends on its interaction with other proteins, implying that no protein functions independently [32][33][34].…”

Section: Tmt Revealed the Heterogeneity Of Stolon Buds In Strawberrymentioning

confidence: 99%

Phenotypic Analysis Combined with Tandem Mass Tags (TMT) Labeling Reveal the Heterogeneity of Strawberry Stolon Buds

Guan

Zhao²,

Qian

et al. 2019

Preprint

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Background: Ramet propagation in strawberry (Fragaria × ananassa) is the most effective way in production. However, the lack of systematically phenotypic observations and high-throughput methods limits our ability to analyze the key factors regulating the heterogeneity in strawberry stolon buds. Results: From observation, we found that the axillary bud located in the first node quickly stepped into dormancy (DSB), after several bract and leaf buds were differentiated. The stolon apical meristem (SAM) degenerated as the new ramet leaf buds (RLB), and the new active axillary stolon buds (ASB) differentiated continually after the differentiation of the first leaf. Using the tandem mass tags (TMT) labeling method, a total of 7,271 strawberry proteins were identified. Between ASB and DSB, the spliceosome DEPs, such as Ser/Arg-rich (SR) and heterogeneous nuclear ribonucleoprotein particle (hnRNP), showed the highest enrichment and high PPI connectivity. This indicated that the differences in DEPs (e.g., SF-3A and PK) at the transcriptional level may be causing the differences between the physiological statuses of ASB and DSB. As expected, the photosynthetic pre-form RLB mainly differentiated from ASB and DSB judging by the DEP enrichment of photosynthesis. However, there are still other specialized features of DEPs between RLB and DSB and between ASB and DSB. The DEPs relative to DNA duplication [e.g., minichromosome maintenance protein (MCM 2, 3, 4, 7)], provide a strong hint of functional gene duplication leading the bud heterogeneity between RLB and DSB. In addition, the top fold change DEP of LSH 10-like might be involved in the degeneration of SAM into RLBs, based on its significant function in modulating the plant shoot initiation. As for RLB/ASB, the phenylpropanoid biosynthesis pathway probably regulates the ramet axillary bud specialization, and further promotes the differentiation of xylem when ASB develops into a new stolon [e.g., cinnamyl alcohol dehydrogenase 1 (CAD1) and phenylalanine ammonia-lyase 1 (PAL1)]. Conclusions: By using phenotypic observation combined with proteomic networks with different types of strawberry stolon buds, the definite dormancy phase of DSB was identified, and the biological pathways and gene networks that might be responsible for heterogeneity among different stolon buds in strawberry were also revealed.

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“…The proteome has an important characteristic difference with the genome, that is, proteins have a direct influence on each other [ 24]. The realization of the function of a protein usually depends on its interaction with other proteins implying that no independent functional protein exists [25][26][27]. Therefore, through comprehensive analysis and evaluation of GO annotation (BP, MF, CC), enrichment in KEGG, and connective degree in PPI, we can predict the core functional DEPs involved in the key metabolic pathways [ 26,28].…”

Section: Tmt Revealed the Heterogeneity Of Stolon Buds In Strawberrymentioning

confidence: 99%

Phenotypic Analysis Combined with Tandem Mass Tags (TMT) Labeling Reveals the Heterogeneity of Strawberry Stolon Buds

Guan

2019

Preprint

View full text Add to dashboard Cite

Background: Ramet propagation in strawberry (Fragaria × ananassa) is the most effective way in production. However, the lack of systematically phenotypic observations and high-throughput methods limits our ability to analyze the key factors regulating the heterogeneity in strawberry stolon buds. Results: From observation, we found that the axillary bud located in the first node quickly stepped into dormancy (DSB), after several bract and leaf buds were differentiated. The stolon apical meristem (SAM) degenerated as the new ramet leaf buds (RLB) and the new active axillary stolon buds (ASB) differentiated continually, after the differentiation of the first leaf. Using tandem mass tags (TMT) labeling method, totally 7,271 strawberry proteins were identified, and were used for further bioinformatics analysis in differentially expressed proteins (DEPs) between the groups of ASB and DSB, RLB and DSB, and RLB and ASB. Between ASB and DSB, the spliceosome DEPs, such as Ser/Arg-rich (SR) and heterogeneous nuclear ribonucleoprotein particle (hnRNP), showed the highest enrichment and high PPI connectivity. This indicated that the differences in DEPs (e.g., SF-3A subunit 2 isoform X1, hnRBP C1827.05c, and PK, cytosolic isozyme) at the transcriptional level may be causing the differences between the physiological statuses of ASB and DSB. As expected, the photosynthetic pre-form RLB mainly differentiated from ASB and DSB judging by the DEP enrichment of photosynthesis. However, there are still other specialized features of DEPs between RLB and DSB and between ASB and DSB. The DEPs relative to DNA duplication [e.g., minichromosome maintenance protein (MCM 2, 3, 4, 7)], provide a strong hint of functional gene duplication leading the bud heterogeneity between RLB and DSB. In addition, the top fold change in DEP LSH 10-like protein might be involved in the degeneration of SAM into RLBs. As for RLB/ASB, the phenylpropanoid biosynthesis pathway probably regulates the ramet axillary bud specialization, and further promotes the differentiation of xylem when ASB develops into a new stolon [e.g., cinnamyl alcohol dehydrogenase 1 (CAD1) and phenylalanine ammonia-lyase 1 (PAL1)]. Conclusions: The definite dormancy phase of DSB, and the biological pathways and gene networks that might be responsible for stolon buds heterogeneity were also revealed.

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Protein–Protein Interactions in Plant Thioredoxin Dependent Systems

Cited by 6 publications

References 99 publications

The Interaction of Ferredoxin with Ferredoxin-Dependent Enzymes

The Interaction of Ferredoxin with Ferredoxin-Dependent Enzymes

Phenotypic Analysis Combined with Tandem Mass Tags (TMT) Labeling Reveal the Heterogeneity of Strawberry Stolon Buds

Phenotypic Analysis Combined with Tandem Mass Tags (TMT) Labeling Reveals the Heterogeneity of Strawberry Stolon Buds

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