Protein-protein interactions of human viruses

Goodacre, Norman; Devkota, Prajwal; Bae, Eunhae H.; Wuchty, Stefan; Uetz, Peter

doi:10.1016/j.semcdb.2018.07.018

Cited by 41 publications

(30 citation statements)

References 107 publications

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“…Studying virus-host protein–protein interactions is an important strategy to better understand the functions of viral proteins [ 31 , 32 ]. Regarding paramyxovirus M protein and host protein interactions, it has been reported that the interaction of the Nipah and Hendra virus M proteins with AP3B1 protein promotes virus-like particle (VLP) production [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Chicken bromodomain-containing protein 2 interacts with the Newcastle disease virus matrix protein and promotes viral replication

Duan

Han

Zhou

et al. 2020

Vet Res

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Bromodomain-containing protein 2 (BRD2) is a nucleus-localized serine-threonine kinase that plays pivotal roles in the transcriptional control of diverse genes. In our previous study, the chicken BRD2 (chBRD2) protein was found to interact with the Newcastle disease virus (NDV) matrix (M) protein using a yeast two-hybrid screening system, but the role of the chBRD2 protein in the replication of NDV remains unclear. In this study, we first confirmed the interaction between the M protein and chBRD2 protein using fluorescence co-localization, co-immunoprecipitation and pull-down assays. Intracellular binding studies indicated that the C-terminus (aa 264–313) of the M protein and the extra-terminal (ET) domain (aa 619–683) of the chBRD2 protein were responsible for interactions with each other. Interestingly, although two amino acids (T621 and S649) found in the chBRD2/ET domain were different from those in the human BRD2/ET domain and in that of other mammals, they did not disrupt the BRD2-M interaction or the chBRD2-M interaction. In addition, we found that the transcription of the chBRD2 gene was obviously decreased in both NDV-infected cells and pEGFP-M-transfected cells in a dose-dependent manner. Moreover, small interfering RNA-mediated knockdown of chBRD2 or overexpression of chBRD2 remarkably enhanced or reduced NDV replication by upregulating or downregulating viral RNA synthesis and transcription, respectively. Overall, we demonstrate for the first time that the interaction of the M protein with the chBRD2 protein in the nucleus promotes NDV replication by downregulating chBRD2 expression and facilitating viral RNA synthesis and transcription. These results will provide further insight into the biological functions of the M protein in the replication of NDV.

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Section: Discussionmentioning

confidence: 99%

Chicken bromodomain-containing protein 2 interacts with the Newcastle disease virus matrix protein and promotes viral replication

Duan

Han

Zhou

et al. 2020

Vet Res

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“…Protein-protein interactions, either transient or as part of stable complexes, are key to most cellular processes and biological pathways. In recent years, it has in fact become clear that a detailed description of specific protein interaction networks is required for our understanding of many disease states and the development of new drugs (Goodacre et al, 2018;Laddach et al, 2018). Accordingly, experimental approaches dedicated to the identification of proteinprotein interactions are fundamental to understanding complex biological processes.…”

Section: Introductionmentioning

confidence: 99%

Proximity-dependent biotinylation mediated by TurboID to identify protein–protein interaction networks in yeast

Larochelle

Bergeron

Arcand

et al. 2019

Journal of Cell Science

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The use of proximity-dependent biotinylation assays coupled to mass spectrometry (PDB-MS) has changed the field of protein-protein interaction studies. However, despite the recurrent and successful use of BioID-based protein-protein interactions screening in mammalian cells, the implementation of PDB-MS in yeast has not been effective. Here, we report a simple and rapid approach in yeast to effectively screen for proximal and interacting proteins in their natural cellular environment by using TurboID, a recently described version of the BirA biotin ligase. Using the protein arginine methyltransferase Rmt3 and the RNA exosome subunits, Rrp6 and Dis3, the application of PDB-MS in yeast by using TurboID was able to recover protein-protein interactions previously identified using other biochemical approaches and provided new complementary information for a given protein bait. The development of a rapid and effective PDB assay that can systematically analyze protein-protein interactions in living yeast cells opens the way for large-scale proteomics studies in this powerful model organism.

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“…Also, the researchers have little knowledge of the molecular biology of SARS-CoV2 infection [16]. In the present state of art, viral infection mechanism is not fully understood though various proteinprotein interactions (PPIs) of virus and host are known [17,18]. So identifying interactions between the SARS-CoV2 virus proteins and host proteins helps understand the mechanism of viral infection and develop treatments and vaccines [19].…”

Section: Introductionmentioning

confidence: 99%

Spatial Distribution of Amino Acids of the SARS-CoV2 Proteins

Hassan¹,

Rout²

2020

Preprint

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The world is now undergoing through a global emergency due to COVID-19 which needs immediate remedies in order to strengthen the healthcare facility to save the nations. Looking towards to the remedies, research on different aspects including the genomic and proteomic level characterizations of the SARS-CoV2 are necessarily important. In this present study, the spatial representation/composition of twenty amino acids across the primary protein sequences of SARS-CoV2 have been looked into through different parameters viz. Shannon entropy, Hurst exponent in order to fetch the autocorrelation and amount of information over the spatial representations. Also frequency distribution of each of the amino acids over the protein sequences have been chalked out.

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Protein-protein interactions of human viruses

Cited by 41 publications

References 107 publications

Chicken bromodomain-containing protein 2 interacts with the Newcastle disease virus matrix protein and promotes viral replication

Chicken bromodomain-containing protein 2 interacts with the Newcastle disease virus matrix protein and promotes viral replication

Proximity-dependent biotinylation mediated by TurboID to identify protein–protein interaction networks in yeast

Spatial Distribution of Amino Acids of the SARS-CoV2 Proteins

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