Protein recovery using gas–liquid dispersions

Enrichment behavior of immunoglobulin by foam fractionation using response surface methodology

“…Like pure water, dilute aqueous solutions have a polar nature and the gas-liquid interface formed between such solutions and gases is of a non-polar nature. The ability of dissolved molecules to transfer from the bulk of a polar liquid to a non-polar interface depends either on the possession by these molecules of an hydrophobic and an hydrophilic part or on them being hydrophobic [ADAMSON 1960, KISHIMOTO 1962, LEMLICH 1972b transfer process is also known as a partition process and is directly connected to the physicochemical properties of the molecules [LEMLICH 1972a, NOBLE, et al 1998, NORD 2000.…”

Selective recovery of lactate dehydrogenase using affinity foam

“…Recently, colloidal gas aphrons (CGAs), i.e., the microbubbles generated by vigorous stirring of a surfactant solution, have attracted attention as yet another tool for separation of proteins (Fernandes et al, 2002;Jauregi and Varley, 1998;Noble et al, 1998;. The microbubbles (10±100 lm), consisting of a gaseous inner core surrounded by a thin, multilayered surfactant ®lm (Jauregi et al, 2000;Sebba, 1987), provide a high interfacial area for adsorption of molecules, and their high buoyancy leads to a quick and simple separation from the bulk solution .…”

Nanobubbles are not a Superficial Matter

“…[84,85] Given the tendency of ions to show either enhanced or reduced concentrations at the air-water interface, [15][16][17][18][19][20][21] it seems likely that nanobubbles will acquire a surface charge, which will then influence the energetics of their potential interactions with biological surfaces, just as is the case for nanoparticles. [86][87][88][89] The presence of nanobubbles at surfaces may also have hydrodynamic implications, especially if there is focused gas flow out of the bubble apex.…”