Protein repellent polyurethane-urea surfaces by chemical grafting of hydroxyl-terminated poly(ethylene oxide): effects of protein size and charge

Archambault, Jacques; Brash, John L.

doi:10.1016/j.colsurfb.2003.09.004

Cited by 96 publications

(90 citation statements)

References 22 publications

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“…[22][23][24][25][26][27] The covalent grafting of PEG onto a variety of substrates, including silicon, 24,28 polyurethane-urea, 29 glass, 30,31 fluorinated ethylene-propylene copolymers, 32 and polysulfone membranes, 33 has been reported along with a quite satisfactory protein-repellent effect. It may be anticipated that the proteinrepellent properties of PEG would prevent an extracellular matrix from being formed and, thus, cells from adhering.…”

Section: Introductionmentioning

confidence: 99%

Improved Performances of Intraocular Lenses by Poly(ethylene glycol) Chemical Coatings

et al. 2007

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Cataract surgery is a routine ophthalmologic intervention resulting in replacement of the opacified natural lens by a polymeric intraocular lens (IOL). A main postoperative complication, as a result of protein adsorption and lens epithelial cell (LEC) adhesion, growth, and proliferation, is the secondary cataract, referred to as posterior capsular opacification (PCO). To avoid PCO formation, a poly(ethylene glycol) (PEG) chemical coating was created on the surface of hydrogel IOLs. Attenuated total reflectance Fourier transform infrared spectroscopy, “captive bubble” and “water droplet” contact angle measurements, and atomic force microscopy analyses proved the covalent grafting of the PEG chains on the IOL surface while keeping unchanged the optical properties of the initial material. A strong decrease of protein adsorption and cell adhesion depending on the molar mass of the grafted PEG (1100, 2000, and 5000 g/mol) was observed by performing the relevant in vitro tests with green fluorescent protein and LECs, respectively. Thus, the study provides a facile method for developing materials with nonfouling properties, particularly IOLs.

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Section: Introductionmentioning

confidence: 99%

Improved Performances of Intraocular Lenses by Poly(ethylene glycol) Chemical Coatings

et al. 2007

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“…For example, PEO has been bonded to polystyrene and lowdensity polyethylene surfaces by use of electron beam irradiation, 6 incorporated into a polyurethane matrix material, 7 or chemically grafted to a surface. 8 Also, the adsorption of proteins and adhesion of mammalian cells were highly reduced by covalent attachment of zwitterionic polymers such as poly(sulfobetaine methacrylate) and poly(carboxybetaine methacrylate). 9 Polymer chains attached by one end to a surface or interface with a density of attachment points high enough to obligate the chains to stretch along the normal to the surface or interface are so-called polymer brushes, as opposed to the so-called mushroom regime.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Surface-Grafted Polyacrylamide Brushes and Their Inhibition of Microbial Adhesion

et al. 2007

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A method is presented to prevent microbial adhesion to solid surfaces exploiting the unique properties of polymer brushes. Polyacrylamide (PAAm) brushes were grown from silicon wafers by atom transfer radical polymerization (ATRP) using a three-step reaction procedure consisting of immobilization of a coupling agent γ-aminopropyltriethoxysilane, anchoring of an ATRP initiator 4-(chloromethyl)benzoyl chloride, and controlled radical polymerization of acrylamide. The surfaces were characterized by X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, ellipsometry, and contact-angle measurements. The calculated grafting density pointed to the presence of a dense and homogeneous polymer brush. Initial deposition rates, adhesion after 4 h, and detachment of two bacterial strains (Staphylococcus aureus ATCC 12600 and Streptococcus salivarius GB 24/9) and one yeast strain (Candida albicans GB 1/2) to both PAAm-coated and untreated silicon surfaces were investigated in a parallel plate flow chamber. A high reduction (70−92%) in microbial adhesion to the surface-grafted PAAm brush was observed, as compared with untreated silicon surfaces. Application of the proposed grafting method to silicone rubbers may offer great potential to prevent biomaterials-centered infection of implants.

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“…3,10 The most prominent example of a protein-resistant surface coating is a brush of poly(ethylene oxide) (PEO) where one end of the PEO chains is chemically bound to the solid substrate. [11][12][13][14][15] The protein resistance of such brushes may be explained by steric repulsions. When a protein molecule approaches a brush of hydrophilic long flexible polymer chains, the brush will be compressed leading to an unfavourable reduced conformational entropy of the polymer chains.…”

Section: Introductionmentioning

confidence: 99%

Salt-induced protein resistance of polyelectrolyte brushes studied using fluorescence correlation spectroscopy and neutron reflectometry

Czeslik

Jackler

Hazlett

et al. 2004

Phys. Chem. Chem. Phys.

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We used two-photon excitation fluorescence correlation spectroscopy (FCS) and neutron reflectometry to study in situ the effect of salt concentration on the degree of protein binding to polyelectrolyte brushes. The binding of bovine serum albumin (BSA) to poly(acrylic acid) (PAA) brushes was characterized at neutral pH values where both the protein and the brushes carry a negative charge. Spherical PAA brush particles were used in the FCS experiments, whereas a planar PAA brush served as protein substrate in the neutron reflectometry experiments. It has been found that BSA binds strongly to both the spherical and the planar PAA brushes under electrostatic repulsion at low ionic strength. The BSA volume fraction profile, as determined from the neutron reflectivities, indicates a deep penetration of the BSA molecules into the PAA brush. However, the analysis of the FCS data reveals that the protein affinity of the spherical PAA brush particles decreases drastically when increasing the concentration of sodium chloride to a few 100 mM. This observation is in line with the measured neutron reflectivities of the planar PAA brush. The reflectivity curve obtained in the absence of protein is virtually overlapping with that measured when the PAA brush is in contact with a BSA solution but containing 500 mM sodium chloride which suggests protein resistance of the planar PAA brush at this elevated salt concentration. The results of this study provide evidence for a new kind of protein-resistant interfaces. Whereas protein binding to the PAA brush is likely to be dominated by the release of counterions, this driving force vanishes as the ionic strength of the solution is raised and protein molecules are repelled from the interface by steric interactions. In a general view, the ''switching'' of the protein affinity of a PAA brush by varying the ionic strength of the protein solution over a relatively small range may appear to be useful for biotechnological applications.

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Protein repellent polyurethane-urea surfaces by chemical grafting of hydroxyl-terminated poly(ethylene oxide): effects of protein size and charge

Cited by 96 publications

References 22 publications

Improved Performances of Intraocular Lenses by Poly(ethylene glycol) Chemical Coatings

Improved Performances of Intraocular Lenses by Poly(ethylene glycol) Chemical Coatings

Synthesis and Characterization of Surface-Grafted Polyacrylamide Brushes and Their Inhibition of Microbial Adhesion

Salt-induced protein resistance of polyelectrolyte brushes studied using fluorescence correlation spectroscopy and neutron reflectometry

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