Protein secretion in Bacillus species.

Simonen, M; Palva, I

doi:10.1128/mmbr.57.1.109-137.1993

Cited by 239 publications

(156 citation statements)

References 247 publications

(371 reference statements)

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“…Transport by the Sec pathway may be precluded if the protein folds at all tightly, and bacterial Sec systems are indeed unable to export a large proportion (in fact the majority) of cytoplasmic or heterologous proteins tested (e.g. [27]). The same problem may apply to those proteins that have arisen since the initial endosymbiotic events, such as 23K, 16K, PSI-N, PSII-T and P16.…”

Section: Implications For the Evolution Of The Tat Pathwaymentioning

confidence: 99%

Different lumen‐targeting pathways for nuclear‐encoded versus cyanobacterial/plastid‐encoded Hcf136 proteins

et al. 2000

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Lumenal proteins are transported across the thylakoid membrane by two very different pathways: Sec-dependent or twin-arginine translocase (Tat)-dependent, where the substrate protein can be transported in a folded state. We present the first evidence that a given protein can be targeted by different pathways in different organisms. Arabidopsis Hcf136 is targeted exclusively by the Tat pathway in pea chloroplasts and no Secdependent transport is evident even when the twin-arginine is replaced by twin-lysine. However, twin-arginine motifs are absent from the presequences of Hcf136 proteins encoded by plastid or cyanobacterial genomes, strongly implying translocation by another pathway (presumably Sec). We suggest that the Hcf136 protein was transferred to the Tat pathway when the gene became incorporated into the nuclear genome, possibly due to the tighter folding associated with the more involved, posttranslational targeting pathway.z 2000 Federation of European Biochemical Societies.

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Section: Implications For the Evolution Of The Tat Pathwaymentioning

confidence: 99%

Different lumen‐targeting pathways for nuclear‐encoded versus cyanobacterial/plastid‐encoded Hcf136 proteins

et al. 2000

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“…B. subtilis and closely related bacilli secrete industrially important enzymes directly into the growth medium at concentrations in excess of 10 g/1 and this has provided the basis of much of the commercial interest in these bacteria [2,16]. However, attempts to use B. subtilis to secrete heterologous proteins at commercially significant concentrations have, with few exceptions, met with little success.…”

Section: Introductionmentioning

confidence: 99%

Sequencing and functional analysis of the genome of Bacillus subtilis strain 168

Harwood

Wipat

1996

FEBS Letters

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The international programme to sequence the 4.2 Mb genome of Bacillus subtilis, a model Gram-positive bacterium, is a joint project involving European, Japanese and US research groups. To date ca. 3.0 Mb of the genome has been sequenced, with the remaining 1.2 Mb expected to be completed in 1997. The amenability of B. subtilis to genetic manipulation, combined with the availability of extensive expertise on its biochemistry and physiology, makes this bacterium a valuable organism in which to investigate the properties of genes for which functions cannot be readily ascribed by standard methods.

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“…Both organisms are attractive for this purpose because of their apathogenity and their classification as GRAS organisms (generally regarded as save). Moreover, their easy cultivation and their high natural capacity to secrete proteins into the growth medium qualify them for the industrial overproduction of homologous or heterologous proteins (Simonen and Palva, 1993). For the control of the physiological state and the productivity of the production cells efficient analysis tools are of great interest.…”

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confidence: 99%

ECB12: 12th European Congess on Biotechnology

2005

Journal of Biotechnology

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Abstracts 3 Systems biology: Transforming biology and medicine Leroy Hood President, Institute for Systems Biology, Seattle, Washington, USA The advent of the Human Genome Project has transformed biology by providing a genetics parts list of all genes and proteins, by fueling the contention biology is an informational science, and by catalyzing the emergence of biological information (e.g., rapid DNA sequencing or DNA chips). From this has emerged a new approach to biology termed systems biology-centered on the idea one can study biological systems by delineating the relationships of all of their component elements-and, hence, come to understand the resulting systems properties. I will discuss these important points, give several examples of systems approaches, and conclude by discussing the profound change systems biology will engender in medicine-moving us toward a predictive, preventive, and personalized medicine. 4Interpreting the human and C. elegans genomes

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Protein secretion in Bacillus species.

Cited by 239 publications

References 247 publications

Different lumen‐targeting pathways for nuclear‐encoded versus cyanobacterial/plastid‐encoded Hcf136 proteins

Different lumen‐targeting pathways for nuclear‐encoded versus cyanobacterial/plastid‐encoded Hcf136 proteins

Sequencing and functional analysis of the genome of Bacillus subtilis strain 168

ECB12: 12th European Congess on Biotechnology

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