Protein separation by affinity extraction with reversed micelles of Span 85 modified with Cibacron Blue F3G-A

Temperature Effects on Micelle Formation and Particle Charging with Span Surfactants in Apolar Media

“…These species share the same sorbitan headgroup, while varying the tail length, tail saturation, and number of tails. Current studies have used reverse micelles of Span 60 and Span 85 as means of protein extraction. − Other studies have used Span 80 reverse micelles to synthesize mesoporous titania membranes for ultrafiltration purposes . Many recent studies have looked at the ability of Span surfactants to charge particles in apolar solvents, measuring the magnitudes and signs of the particle electrophoretic mobilities. − Given the wide array of applications of these compounds, many different operating conditions are used.…”

Effects of Reverse Micellar Structure on the Particle Charging Capabilities of the Span Surfactant Series

“…These compounds share the same sorbitan headgroup, while varying in tail length, tail saturation, and number of tails, as shown in Figure . Current studies have used inverse micelles of Span 60 and Span 85 − as means of protein extraction from solution, while other studies have used inverse micelles of Span 80 to synthesize mesoporous titania membranes for ultrafiltration purposes . Many recent studies have looked at the ability of Span surfactants to charge particles in apolar solvents, measuring the magnitudes and signs of the particle electrophoretic mobilities. ,,,, However, most studies have looked only at how the chemistry of the surfactant headgroup affects the charging behavior. − ,, The authors’ most recent study has shown that the magnitude of particle charge, of 30 nm magnesia particles, increases as the tail length of the Span species increases.…”

Water Encapsulation by Nanomicelles

“…A fine-tuning of the experimental conditions allowed formation of a propofol trimer and tetramer with a water molecule and to determine the structure of the aggregates. Because of their important biological role, several applications have been found for RMs: they can be used as reaction systems for enzymatic catalysis, [5,6] drug delivery, [7,8] solvent-based extraction of proteins, [9,10] industrial processes, [11] microenvironment for discovery of protein structures, [12] protein refolding, [13,14] or even treated as models of membrane systems for the separation of proteins, [15] etc. Interpretation of the spectra in the light of high-level calculations allowed determination of the clusters structure and demonstration that the trimer of propofol with a water molecule forms cyclic hydrogen-bond networks but, on the other hand, the tetramer is big enough to encapsulate the water molecule inside its hydrophilic core.…”

“…[1,2] RMs are thus able to encapsulate microscopic pools of water while the apolar part remains in contact with an organic solvent. Because of their important biological role, several applications have been found for RMs: they can be used as reaction systems for enzymatic catalysis, [5,6] drug delivery, [7,8] solvent-based extraction of proteins, [9,10] industrial processes, [11] microenvironment for discovery of protein structures, [12] protein refolding, [13,14] or even treated as models of membrane systems for the separation of proteins, [15] etc. Because of their important biological role, several applications have been found for RMs: they can be used as reaction systems for enzymatic catalysis, [5,6] drug delivery, [7,8] solvent-based extraction of proteins, [9,10] industrial processes, [11] microenvironment for discovery of protein structures, [12] protein refolding, [13,14] or even treated as models of membrane systems for the separation of proteins, [15] etc.…”