Protein Separations Using Colloidal Magnetic Nanoparticles

Bucak, Şeyda; Jones, Deverraux A.; Laibinis, Paul E.; Hatton, T. Alan

doi:10.1021/bp0200853

Cited by 302 publications

(192 citation statements)

References 16 publications

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“…[1][2][3][4][5] For example, antibody and glass functionalized SPM beads are now widely used for the isolation of specific cell types and nucleic acids, respectively. 6,7 High-gradient magnetophoresis (HGM) is 25 typically used to collect the SPM beads from complex aqueous solutions, i.e., a high magnetic field and field gradients generated by a magnet are applied to the suspension to remove the SPM beads.…”

Section: Introductionmentioning

confidence: 99%

Flow enhanced non-linear magnetophoretic separation of beads based on magnetic susceptibility

Kilinc

Ran

et al. 2013

Lab Chip

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Magnetic separation provides a rapid and efficient means of isolating biomaterials from complex mixtures based on their adsorption on superparamagnetic (SPM) beads. Flow enhanced non-linear magnetophoresis (FNLM) is a high-resolution mode of separation in which hydrodynamic and magnetic fields are controlled with micron resolution to isolate SPM beads with specific physical properties. In this article we demonstrate that a change in the critical frequency of FNLM can be used to identify beads with 10 magnetic susceptibilities between 0.01 and 1.0 with a sensitivity of 0.01 Hz -1 . We derived an analytical expression for the critical frequency that explicitly incorporates the magnetic and non-magnetic composition of a complex to be separated. This expression was then applied to two cases involving the detection and separation of biological targets. This study defines the operating principles of FNLM and highlights the potential for using this technique for multiplexing diagnostic assays and isolating rare cell 15 types.

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Section: Introductionmentioning

confidence: 99%

Flow enhanced non-linear magnetophoretic separation of beads based on magnetic susceptibility

Kilinc

Ran

et al. 2013

Lab Chip

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“…However, such solid systems can have slow mass transport and complex recycling processes. A better option is to use dispersed nanoparticles, which are homogeneously distributed in solution; such a system has favorable mass transport to surfaces and can permit magnetic capture of depleted materials [10]. In addition, dispersed sorbents avoid many of the classical problems of filtration related to occluding and fouling of packed columns and membranes.…”

Section: Introductionmentioning

confidence: 99%

The effect of nanocrystalline magnetite size on arsenic removal

Mayo

Yavuz

Yean

et al. 2007

Science and Technology of Advanced Materials

446

179

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Higher environmental standards have made the removal of arsenic from water an important problem for environmental engineering. Iron oxide is a particularly interesting sorbent to consider for this application. Its magnetic properties allow relatively routine dispersal and recovery of the adsorbent into and from groundwater or industrial processing facilities; in addition, iron oxide has strong and specific interactions with both As(III) and As(V). Finally, this material can be produced with nanoscale dimensions, which enhance both its capacity and removal. The objective of this study is to evaluate the potential arsenic adsorption by nanoscale iron oxides, specifically magnetite (Fe 3 O 4 ) nanoparticles. We focus on the effect of Fe 3 O 4 particle size on the adsorption and desorption behavior of As(III) and As(V). The results show that the nanoparticle size has a dramatic effect on the adsorption and desorption of arsenic. As particle size is decreased from 300 to 12 nm the adsorption capacities for both As(III) and As(V) increase nearly 200 times. Interestingly, such an increase is more than expected from simple considerations of surface area and suggests that nanoscale iron oxide materials sorb arsenic through different means than bulk systems. The desorption process, however, exhibits some hysteresis with the effect becoming more pronounced with small nanoparticles. This hysteresis most likely results from a higher arsenic affinity for Fe 3 O 4 nanoparticles. This work suggests that Fe 3 O 4 nanocrystals and magnetic separations offer a promising method for arsenic removal. r

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“…Their magnetic features have found widespread uses in applications as diverse as environmental remediation, magnetic recording, biomacromolecule separation, catalyst separation, drug/gene delivery and release, and magnetic resonance imaging. [15][16][17][18] In the past few years, multifunctional composite materials have attracted inevasible attention of scientists.…”

Section: Introductionmentioning

confidence: 99%