Protein status of holshtinized black and white cattle

Себежко, О. И.; Mayer, RJ; Tarasenko, E. I.; Marenkov, V. G.; Garth, V. V.

doi:10.1051/bioconf/20213606023

Cited by 3 publications

(1 citation statement)

References 3 publications

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“…Показатели продуктивности крупного рогатого скота связаны с интерьерными особенностями животных, включая генетически обусловленные реакции азотистого и белкового обменов [1,2]. Аспартатаминотрансфераза (АсТ), или глутамат оксалоацетат трансаминаза (GOT), представляет собой пиридоксальфосфат-зависимый трансаминазный фермент, который участвует в азотистом обмене у животных и человека, катализируя обратимый перенос α-аминогруппы между аминокислотами во многих органах и тканях [3].…”

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Hereditary definiteness of aspartate aminotransferase level in Holstein cattle

Себежко

Konovalova

Korotkevich

et al. 2023

Vestn. Novosib. gos. agrar. univ.

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Farm animals’ growth, development and realization of productive qualities are closely interrelated by enzymatic transformations of amino acids, proteins and other complex nitrogenous compounds. Aspartate aminotransferase (AsT) is involved in the metabolism of amino acids in the cycles of urea, tricarboxylic acids and gluconeogenesis. The level of AsT in the blood depends on many paratypical and genetic factors. The paper presents the results of a biochemical analysis of the AsT content in the blood serum of Holstein bulls aged 12-13 months, who are descendants of 4 breeding bulls. Blood sampling was carried out in animals with a live weight of 330-365 kg, bred in the conditions of a large livestock enterprise located in Western Siberia. The level of AsT in the blood serum of animals was determined by the Reitman-Frenkel colourimetric method using reagents “Transaminase AsT Novo” (manufacturer “Vector-Best” Russia). It was found that the average value of the AsT content in the groups of sons was lower than the generally accepted physiological norm and, for all descendants, was 22.44 ± 1.29 E/l. The reference interval for AsT based on the central 95% percentile with 90% confidence intervals was 12.29 (8.67...15.91) - 34.84(31.2... 38.5) E/l. The descendants of different fathers had different phenotypic variability of the indicator and differed in the level of AST. Thus, the descendants of the bull-producer Brio were characterized by a higher level compared to the sons of the bulls Malstrem and Fabio by 1.69 and 1.8 times, respectively. The revealed differences indicate the hereditary determination of the AsT level in Holstein cattle. The strength of the influence of the father’s factor was 0.18.

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Hereditary definiteness of aspartate aminotransferase level in Holstein cattle

Себежко

Konovalova

Korotkevich

et al. 2023

Vestn. Novosib. gos. agrar. univ.

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Nitrogen metabolism in blackmoiled cattle of Kuzbass

Tarasenko,

Sebezhko

2024

Vestn. Novosib. gos. agrar. univ.

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A research analysis of nitrogen metabolism in black-and-white cattle was carried out in the conditions of the Kuzbass region. Healthy animals at the age of 2nd lactation with a productivity level from 7300 to 10500 kg were selected for the experimental group. Laboratory studies were carried out at the Department of Veterinary Genetics and Biotechnology of the Novosibirsk State Agrarian University. Indicators of nitrogen metabolism in blood serum were assessed photometrically on a biochemical semi-automatic analyzer Photometer 5010 V5+. Reagent kits from Vector-Best and Olvex-Diagnosticum were used. When biometric data processing, standard descriptive statistics techniques and robust indicators were used. The Anderson-Darling test was used to assess the normality of data distribution. .When calculating correlation relationships, the Pearson coefficient was used. Calculations were performed in the “RStudio” programming environment. The obtained average values of nitrogen metabolism indicators were within the physiological norm. The strongest positive associations were observed between urea levels and uric acid, and urea and creatinine. No connection was found between other indicators of nitrogen metabolism. There were no differences in milk yield between cows with high and low levels of nitrogen metabolism. Graphs of the range of nitrogen content in black-and-white cows during lactation are presented. Calculations of reference indicators based on data from Kuzbass dairy cattle make it possible to more accurately assess the clinical condition of animals and timely detect deviations from established standard values. It is also necessary to take into account reference limits when monitoring the quality and completeness of cattle.

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Assessment of the influence of the metabolitic probiotic Biosib METAPRO on protein metabolism indicators in broiler chickens

Shvydkov,

Sebezhko,

Kalmykova

et al. 2024

Vestnik of the Russian agricultural science

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The paper presents data on the study of the influence of the metabolic probiotic (metaprobiotic) Biosib METAPRO, developed based on a complex of cultures Bifidobacterium longum and Lactobacillus plantarum at LLC “Sibbiopharm”, on the indicators of protein metabolism in broiler chickens. The study was conducted on Ross 308 cross chickens from 6 days to 35 days of age, using four groups: control and three experimental groups, to which Biosib METAPRO was additionally administered in different doses. The quantitative assessment of protein metabolism indicators in the blood serum of birds was performed photometrically using Vector-Best reagent kits. It was established that the levels of total protein, albumin, globulin, albumin/globulin ratio, alanine aminotransferase (ALT), aspartate aminotransferase (AST), de Ritis coefficient, and creatinine in broiler chickens were within the generally accepted physiological norms for birds of this age or showed insignificant deviations. Statistical analysis revealed a significant influence of the metaprobiotic on total protein, A/G ratio, ALT, AST, de Ritis coefficient, and creatinine. The most pronounced changes were observed in chickens receiving Biosib METAPRO at a dose of 0.05%. The greatest number of differences was found between the control and the 1st experimental (Biosib METAPRO at a dose of 0.05%) groups. When administering the preparation at this dosage to broiler chickens, the level of total protein increases by 9.97 g/l, there is a change in A/G ratio, an increase in ALT activity by 1.32 mmol/l⋅h, a decrease in AST by 3.76 mmol/l⋅h, normalization of the de Ritis coefficient, and an increase in the level of creatinine by 13.2 µmol/l (p0.05). The results of the study demonstrate the positive influence of the metabolic probiotic Biosib METAPRO on protein metabolism in broiler chickens, which may contribute to increasing the efficiency and sustainability of poultry meat production.

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Protein status of holshtinized black and white cattle

Cited by 3 publications

References 3 publications

Hereditary definiteness of aspartate aminotransferase level in Holstein cattle

Hereditary definiteness of aspartate aminotransferase level in Holstein cattle

Nitrogen metabolism in blackmoiled cattle of Kuzbass

Assessment of the influence of the metabolitic probiotic Biosib METAPRO on protein metabolism indicators in broiler chickens

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