Protein-stimulated enrichment of human platelet membranes in linoleylphosphatidylcholines. Effect upon adenylate cyclase and fluidity

Chambaz, Jean; Pépin, Dominique; Robert, Annie; Wolf, Claude; Béréziat, G.

doi:10.1016/0005-2736(83)90417-0

Cited by 13 publications

(3 citation statements)

References 64 publications

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“…Cyclic adenosine monophosphate (cAMP) plays a significant role in the maintenance of oocyte meiotic arrest in several mammalian species (mouse, Cho et al, 1974;rat, Magnusson & Hillensjö, 1977; pig, Rice & McGaughey, 1981, Racowsky, 1985acow, Ball et al, 1983;hamster, Racowsky, 1985b;cow, Homa, 1988). Incorporation of unsaturated fatty acids into membrane phospholipids increases basal and stimulated activity of adenylate cyclase (Engelhard et al, 1978;Colard et al, 1980;Poon et al, 1981;Chambaz et al, 1983). The stimulatory effect is specific for incorporation of linoleic acid (Colard et al, 1980;Chambaz et al, 1983).…”

Section: Discussionmentioning

confidence: 99%

“…Incorporation of unsaturated fatty acids into membrane phospholipids increases basal and stimulated activity of adenylate cyclase (Engelhard et al, 1978;Colard et al, 1980;Poon et al, 1981;Chambaz et al, 1983). The stimulatory effect is specific for incorporation of linoleic acid (Colard et al, 1980;Chambaz et al, 1983). Evidence supports the existence of adenylate cyclase in the mammalian oocyte plasma membrane (Racowsky, 1985a, b), and it is therefore tempting to speculate that the inhibitory effect of linoleic acid on spontaneous breakdown of germinal vesicles in bovine oocytes may be due, in part, to its specific stimulatory effect on activity of adenylate cyclase.…”

Section: Discussionmentioning

confidence: 99%

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Changes in linoleic acid during follicular development and inhibition of spontaneous breakdown of germinal vesicles in cumulus-free bovine oocytes

Homa

Brown²

1992

Reproduction

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Summary. The fatty-acid composition of follicular fluid from small and large developing follicles was analysed and the effects of saturated and unsaturated fatty acids on spontaneous breakdown of germinal vesicles were investigated. Fatty acids were bound to bovine serum albumin and cultured with occytes at 100 \g=m\mol/l. Linoleic acid (18:2) was the only fatty acid tested that significantly inhibited breakdown of germinal vesicles (P < 0\m=.\01). The effect was dose-dependent and was greatest at 50 \g=m\molfatty acid/1 (% breakdown of control, 81\m=.\1\ m=+-\6\m=.\8 vs. 50\g=m\mol linoleic acid/1, 35\m=.\4\ m=+-\7\ m=. \ 3; P < 0\m=.\02). Linoleic acid was the major fatty acid, constituting about a third of the total fatty acid in the follicular fluid; followed by 18\m=.\9 \ m=+-\ 1\m=.\0%and 16\m=.\9 \ m=+-\ 1\m=.\3%oleic acid (18:1) in small and large follicles, respectively. Saturated fatty acids accounted for <30% of the total fatty acid composition. There was a marked absence of tetraenoic acids in small and large follicles. Proportions of linoleic acid were significantly lower in follicular fluid from large follicles (31\m=.\1 \m=+-\1\m=.\2%of total fatty acid) than from small follicles (34\m=.\8 \m=+-\0\m=.\7%of total fatty acid) (P < 0\m=.\05) and there was a significant inverse correlation between follicle diameter and percentage of linoleic acid in the follicular fluid (r= \m=-\0\m=.\6966; P < 0\m=.\05). There was no significant alteration in any other fatty acid during follicular development. The results suggest that (i) a critical relative concentration of linoleic acid may be required for maintenance of meiotic arrest in bovine oocytes and (ii) the decline in the proportion of linoleic acid during follicular development may contribute to the relief of the inhibition of breakdown of germinal vesicles.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Changes in linoleic acid during follicular development and inhibition of spontaneous breakdown of germinal vesicles in cumulus-free bovine oocytes

Homa

Brown²

1992

Reproduction

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“…Membrane micro viscosity, which depends on the nature of PUFA chains in the phospholipids and on the proportion of cholesterol, affects the interaction between the receptor, regulator and effector components of the AC complex, but in a not readily predictable manner (e.g. Sinensky, Minneman & Molinoff, 1979;Salesse, Garnier & Daveloos, 1982;Chambaz et al, 1983). LPX will tend to increase membrane microviscosity but also to cause degradation ofthe membrane structure; in synaptic membranes, but not in membrane fractions from liver, heart muscle or erythrocytes, LPX has been reported to result in activation of AC (Baba et al, 1981).…”

Section: Possible Consequences Of Altered Membrane Propertiesmentioning

confidence: 99%

Neurochemical Approaches to the Pathogenesis of Down's Syndrome

Balázs¹,

Brooksbank²

1985

J intellect Disabil Res

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Characterization and analysis of the subclasses and molecular species of choline phosphoglycerides from porcine heart by successive chemical hydrolyses and reverse phase high-performance liquid chromatography

Shaikh

1990

Mol Cell Biochem

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Choline phosphoglycerides (CPG) represent the major fraction of heart phospholipids. Since depletion of membrane phospholipids and accumulation of lyso-compounds, particularly lysophosphatidylcholines, have been implicated in arrhythmogenesis, it was of great interest to study the composition of this major phospholipid fraction of the heart at a molecular level in an established animal model. The data presented here describe the first report on the detailed chemical examination of CPG and resolution, characterization and quantitative analysis of the molecular species of this phospholipid fraction from porcine heart by high performance liquid chromatography (HPLC). This fraction constitutes 37.5 +/- 0.7% (n = 21) of the total phospholipids and upon successive mild acid and alkaline hydrolyses revealed the presence of essentially three subclasses: diacyl-, alkenylacyl-, and alkylacyl glycerophosphorylcholines, in a relative abundance of 57.7 +/- 2.2% (n = 8), 37.3 +/- 1.3% (n = 8) and 4.6 +/- 0.2% (n = 8), respectively. The fourth subclass, dialkyl CPG was found only in minute amounts (0.43 +/- 0.05%, n = 8) and the presence of dialkenyl and alkenylalkyl analogues could not be detected. Alternatively, by converting the CPG fraction to benzoate derivatives after phospholipase C digestion, it was possible to isolate and quantitate subclass composition by TLC/spectroscopy or both subclass compositions and molecular species analysis by HPLC directly by a UV detector online with the column. By these techniques, subclass composition was found to be very similar to that obtained by the chemical hydrolysis technique. By HPLC, up to 25 species can be identified and quantitated in each subclass, their identity being confirmed by gas-liquid chromatography, after their isolation from the column. The analyses showed that up to 74% of the diacyl moiety consisted of 16:0-18:2 (34%), 16:0-18:1 (27%), and 18:0-18:2 (13%) species, while the major species of the alkenylacyl moiety were 16:0-18:2 (44%) 16:0-18:1 (13%), 16:0-20:4 (12%) and 18:1-18:2 (9%) making up more than 75% of the total mass of this subclass. The major molecular species of the alkylacyl moiety was 16:0-18:2, constituting up to 47% of this fraction, while others constituted about 10% (16:0-18:1), 9% (18:1-18:2), 8% (16:0-20:4) and 6% (18:0-18:2), making up 80% of the total mass. The ether chain composition of akylacyl CPG whether determined after isolation of this fraction by the chemical hydrolysis technique or by HPLC was indistinguishable.(ABSTRACT TRUNCATED AT 400 WORDS)

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Protein-stimulated enrichment of human platelet membranes in linoleylphosphatidylcholines. Effect upon adenylate cyclase and fluidity

Cited by 13 publications

References 64 publications

Changes in linoleic acid during follicular development and inhibition of spontaneous breakdown of germinal vesicles in cumulus-free bovine oocytes

Changes in linoleic acid during follicular development and inhibition of spontaneous breakdown of germinal vesicles in cumulus-free bovine oocytes

Neurochemical Approaches to the Pathogenesis of Down's Syndrome

Characterization and analysis of the subclasses and molecular species of choline phosphoglycerides from porcine heart by successive chemical hydrolyses and reverse phase high-performance liquid chromatography

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