Protein‐Structure‐Directed Metal–Organic Zeolite‐like Networks as Biomacromolecule Carriers

Abstract

Fabrication of zeolite‐like metal–organic frameworks (ZMOFs) for advanced applications, such as enzyme immobilization, is of great interest but is a great synthetic challenge. Herein, we have developed a new strategy using proteins as structure‐directed agents to direct the formation of new ZMOFs that can act as versatile platforms for the in situ encapsulation of proteins under ambient conditions. Notably, protein incorporation directs the formation of a ZMOF with a sodalite (sod) topology instead of a non‐porous diamondoid (dia) topology under analogous synthetic conditions. Histidines in proteins play a crucial role in the observed templating effect. Modulating histidine content thereby influenced the resultant MOF product (from dia to dia + sod mixture and, ultimately, to sod MOF). Moreover, the resulting ZMOF‐incorporated proteins preserved their activity even after exposure to high temperatures and organic solvents, demonstrating their potential for biocatalysis and biopharmaceutical applications.

Synthesis of a Reactive Template-Induced Core–Shell PZS@ZIF-67 Composite Microspheres and Its Application in Epoxy Composites

“…Novel hybrid nanomaterials with particular surface chemistry and multidimensional geometric nanoarchitecture may promote advanced materials' rapid evolution. As an important branch of MOFs (metal-organic frameworks), zeolite-like metal-organic frameworks (ZIFs) integrate the advantages of zeolite and MOFs, such as high porosity, cage-like cavities, adjustable pores, designate functions, excellent stability, etc., which caused great application prospects in the fields of efficient catalysis [27,28], energy storage [29,30], gas adsorption separation [31,32], sensor detection [33,34], and immobilized enzymes [35,36]. In recent years, researchers have been focusing on regulating MOFs' morphology and structure, especially making MOFs exist as ultra-thin flakes, which can effectively increase the coordinatively unsaturated metal sites and further improve their application performance.…”

Short‐Time Hydrothermal Treatment of Poplar Wood for the Production of a Lignin‐Derived Polyphenol Antioxidant

“…Because each LPAm olecule consists of 6.2-6.8 aromaticr ings on average, almost every aromatic ring had one PhOH group, which is possibly the reason why LPAw as soluble in water.T he remarkable PhOH content of LPAw ould allow wide applications not only [15] 1105-4191 783-13771.4-3.0 2.2-4.8 12.5-68.5 DAIL [30] 860-1190 540-650 1.5-1.9 0.9-1.8 2.0-5.6 BHT ----0.2, [30] 0.3, [31] 1.1 [15] BHA ----2.4, [31] 11.1 [30] VE [15] ----263. in antioxidant synthesis buta lso in polymer synthesis, for example,for phenolic adhesives and resins. [36] The crude LPAp reparationsw ere tested for antioxidant properties as inhibitors. The DPPH inhibition curve is shown in Figure 6.…”

Embedding Functional Biomacromolecules within Peptide‐Directed Metal–Organic Framework (MOF) Nanoarchitectures Enables Activity Enhancement

“…To date,3 Dz eolitic imidazolate frameworks (ZIFs,c a. 3.5 aperture) are the most widely explored MOFs for hosting bio-entities, [12][13][14][15][16][17][18][19][20] owing to their biocompatible synthesis conditions that shield biomacromolecules against denaturation during the MOF biohybrids formation. The activity of the biomacromolecule after entrapment in a3 D microporous ZIF,h owever, is still far from satisfactory.F or instance,t he biomacromolecules (horseradish peroxidase, lipase,c atalase,e tc.)…”

“…MOFs have great potential for hosting guest molecules compared with traditional materials . Recently, scientists found that biomacromolecules could be entrapped in situ into the growing MOFs via approaches such as coprecipitation and biomineralization . The biomacromolecule@MOF biohybrids allow cell activity to be mimicked .…”

“…[9][10][11] Recently,s cientists found that biomacromolecules could be entrapped in situ into the growing MOFs via approaches such as coprecipitation and biomineralization. [12][13][14][15][16][17][18][19] Thebiomacromolecule@MOF biohybrids allow cell activity to be mimicked. [20] Fore xample,t he ultrahigh porosity of MOFs provides cytomembrane-like structures for hosting biomolecules,a nd the porous MOFs networks allows the selective transport of the substrate that mimics the biotransformation process in living cell.…”