Protein-surface interactions on stimuli-responsive polymeric biomaterials

Cross, M. C.; Toomey, Ryan; Gallant, Nathan D.

doi:10.1088/1748-6041/11/2/022002

Cited by 41 publications

(21 citation statements)

References 123 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Coatings based on thermoresponsive polymers consistently prove to be a valuable foundation for cell sheet engineering purposes [ 1 , 2 , 3 ]. Due to their physical response to changes in temperature under aqueous conditions, such coatings can switch from a rather hydrophobic, protein- and cell-adhesive state into a more hydrophilic, protein- and cell-repellant state upon cooling [ 4 , 5 ]. This phase transition is characterized by an increase in coating hydration, which is usually accompanied by the swelling of the thermoresponsive polymer layer.…”

Section: Introductionmentioning

confidence: 99%

Thermoresponsive Poly(glycidyl ether) Brush Coatings on Various Tissue Culture Substrates—How Block Copolymer Design and Substrate Material Govern Self-Assembly and Phase Transition

Stöbener

Weinhart

2020

Polymers

View full text Add to dashboard Cite

Thermoresponsive poly(glycidyl ether) brushes can be grafted to applied tissue culture substrates and used for the fabrication of primary human cell sheets. The self-assembly of such brushes is achieved via the directed physical adsorption and subsequent UV immobilization of block copolymers equipped with a short, photo-reactive benzophenone-based anchor block. Depending on the chemistry and hydrophobicity of the benzophenone anchor, we demonstrate that such block copolymers exhibit distinct thermoresponsive properties and aggregation behaviors in water. Independent on the block copolymer composition, we developed a versatile grafting-to process which allows the fabrication of poly(glycidyl ether) brushes on various tissue culture substrates from dilute aqueous-ethanolic solution. The viability of this process crucially depends on the chemistry and hydrophobicity of, both, benzophenone-based anchor block and substrate material. Utilizing these insights, we were able to manufacture thermoresponsive poly(glycidyl ether) brushes on moderately hydrophobic polystyrene and polycarbonate as well as on rather hydrophilic polyethylene terephthalate and tissue culture-treated polystyrene substrates. We further show that the temperature-dependent switchability of the brush coatings is not only dependent on the cloud point temperature of the block copolymers, but also markedly governed by the hydrophobicity of the surface-bound benzophenone anchor and the subjacent substrate material. Our findings demonstrate that the design of amphiphilic thermoresponsive block copolymers is crucial for their phase transition characteristics in solution and on surfaces.

show abstract

Section: Introductionmentioning

confidence: 99%

Thermoresponsive Poly(glycidyl ether) Brush Coatings on Various Tissue Culture Substrates—How Block Copolymer Design and Substrate Material Govern Self-Assembly and Phase Transition

Stöbener

Weinhart

2020

Polymers

View full text Add to dashboard Cite

show abstract

“…For example, Jalili et al developed injectable PNIPAM‐ co ‐acrylamide hydrogels capable of releasing DOX in a temperature and magnetic field dependent manner. Multiple stimuli‐responsive hydrogels for drug delivery and release have been reviewed elsewhere . Such materials can be used in 4D bioinks.…”

Section: Potential Bioinks For 4d Bioprintingmentioning

confidence: 99%

Advances and Future Perspectives in 4D Bioprinting

Ashammakhi

Ahadian

Fan

et al. 2018

Biotechnology Journal

198

106

View full text Add to dashboard Cite

Three-dimensionally printed constructs are static and do not recapitulate the dynamic nature of tissues. Four-dimensional (4D) bioprinting has emerged to include conformational changes in printed structures in a predetermined fashion using stimuli-responsive biomaterials and/or cells. The ability to make such dynamic constructs would enable an individual to fabricate tissue structures that can undergo morphological changes. Furthermore, other fields (bioactuation, biorobotics, and biosensing) will benefit from developments in 4D bioprinting. Here, the authors discuss stimuli-responsive biomaterials as potential bioinks for 4D bioprinting. Natural cell forces can also be incorporated into 4D bioprinted structures. The authors introduce mathematical modeling to predict the transition and final state of 4D printed constructs. Different potential applications of 4D bioprinting are also described. Finally, the authors highlight future perspectives for this emerging technology in biomedicine.

show abstract

“…studied gels. Crosslinked PNIPAM gels swell at temperatures lower than about 34 °C and collapse at temperatures higher than this value [15,16]. This type of thermo-responsive behaviour has demonstrated successful drug release.…”

Section: Introductionmentioning

confidence: 98%

Temperature-sensitive Ternary Interpenetrating Polymeric Networks for Potential Gastrointestinal Drug Release

Ekici¹

2017

J. Drug

View full text Add to dashboard Cite

Interpenetrating polymeric network (IPN) structures have been studied extensively as drug carriers to enhance the required therapeutic effect. In this study, IPNs were prepared in the form of cylindrical by using chitosan known to be biocompatible with the body, poly(N-isopropyl acrylamide) (PNIPAM) which has temperature-sensitive nature, and poly(N-vinyl pyrrolidone) (PVP). N,N'-methylenebisacrylamide (MBA) and glutaraldehyde (GA) were selected as crosslinkers to clamp polymer chains. GA were used in different concentration to control the network porous of the IPNs. Dynamic swelling studies were carried out at pH 1.1 and pH 7.4. Pulsatile swelling studies were performed on all IPN hydrogels to determine to what extent the hydrogels would respond to changes in environmental pH and temperature and how fast that response would be. Amoxicillin was loaded into the hydrogels, and released into buffered solutions as a function of pulsatile changes in temperature and pH. Amoxicillin was loaded into the hydrogels, and released into buffered solutions as a function of pulsatile changes in temperature and pH. The final percentage of amoxicillin released is noted to decrease as the content of GA in the hydrogel increases. Data clearly demonstrate that the use of thermosensitive IPN hydrogels can increase the release of drug molecules from hydrophilic IPNs based on PNIPAM. It can be said from these values that IPNs are intelligent hydrogels and can be thought of as potential devices for pH-and temperaturestimulated sustained delivery of drugs into physiological solutions or for other biomedical applications.

show abstract

Protein-surface interactions on stimuli-responsive polymeric biomaterials

Cited by 41 publications

References 123 publications

Thermoresponsive Poly(glycidyl ether) Brush Coatings on Various Tissue Culture Substrates—How Block Copolymer Design and Substrate Material Govern Self-Assembly and Phase Transition

Thermoresponsive Poly(glycidyl ether) Brush Coatings on Various Tissue Culture Substrates—How Block Copolymer Design and Substrate Material Govern Self-Assembly and Phase Transition

Advances and Future Perspectives in 4D Bioprinting

Temperature-sensitive Ternary Interpenetrating Polymeric Networks for Potential Gastrointestinal Drug Release

Contact Info

Product

Resources

About