Protein synthesis during germination of Allomyces macrogynus mitospores

Smith, Beverly A.; Burke, Daniel D.

doi:10.1128/jb.143.3.1498-1500.1980

Cited by 1 publication

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“…The evidence presented in this paper and in a related study (28) indicates that ungerminated mitospores of A. macrogynus contain their own developmental program for early protein synthe-sis required for germination in the form of prepackaged polysomes and stable template mRNA. A subsequent paper will deal with protein synthesis during spore germination (29).…”

Section: Resultsmentioning

confidence: 99%

Pattern of ribonucleic acid synthesis during germination of Allomyces macrogynus mitospores

Smith¹,

Burke²

1980

J Bacteriol

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The relationship between ribonucleic acid (RNA) synthesis and germination of Allomyces macrogynus mitospores was investigated. It was determined that the synthesis of all classes of RNA was initiated during the first 10 min of germination, around the time of encystment. It is during this stage that the membrane of the nuclear cap structure begins to break down, dispersing the cell complement of ribosomes throughout the cytoplasm. After encystment, there was an increase in the rate of synthesis of the four stable RNA species (4S, 5S, 19S, and 27S) which leveled off as the germ tube emerged. Data suggested that messenger RNA was synthesized at an increasing rate during the course of germination. Studies of RNA precursor pool behavior and RNA synthesis in the presence and absence of actinomycin D indicated that no species of RNA, including messenger RNA, was synthesized in the presence of actinomycin D. Further, precursor pool measurement indicated that the apparent increase in the rate of RNA synthesis during germination was largely due to increased specific activity of the RNA precursor pool.

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Section: Resultsmentioning

confidence: 99%