Protein Synthesis during the Initial Phase of the Temperature-Induced Bleaching Response in <i>Euglena gracilis</i>

Ortiz, William

doi:10.1104/pp.93.1.141

Cited by 7 publications

(5 citation statements)

References 17 publications

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“…It has been shown, in vitro, by Brandt and Wiessner (1977) that chloroplast mRNA polymerase IV is completely inhibited at elevated temperatures. Furthermore, in vivo, several changes occur when E. oracilis is exposed to 34-35 ~ C, including a reduced incorporation of [14C]leucine into chloroplast proteins (Brandt 1981) and changes in the chloroplast protein pattern (Ortiz and Wilson 1988;Ortiz 1990). Under the same conditions we also observed a drastic decrease in chloroplast transcription, as measured by 32p incorporation into chloroplast RNAs (data not shown).…”

Section: Discussionsupporting

confidence: 49%

Photocontrol of thylakoid protein synthesis in Euglena: differential post-transcriptional regulation depending on nutritional conditions

Weiss

Houlné

Schantz

1992

Planta

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The expression of three chloroplastic genes, psbA (the gene for the reaction center D1 protein of PSII), psbC (the gene for the P700 apoprotein of PSI) and psaB (the gene for the intermediate antema of PSII), and a nuclear gene, cab (the gene for the chlorophyll a/bbinding protein), has been investigated during chloroplast development in Euglena gracilis Klebs. The polysomal fraction, mRNA stability and protein turnover were analysed under different conditions of cell greening. The results indicate that the main regulatory step for the nuclear and chloroplastic genes was at the translational level when greening of cells took place on a resting medium. When cell greening took place on a medium with balanced phosphate, carbon and nitrogen sources (nutritional medium), the main regulation occurred posttranscriptionally by mobilization of the transcripts onto the polysomes. These results indicate that in E. gracilis, for a given gene, regulation operates at different levels, and that although light is the principal effector in the regulation of the genes involved in chloroplast development, the effect of metabolites should also be considered. Interestingly, it appears that these various regulation levels are similar for the chloroplastic and nucleocytoplasmic compartments.

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Section: Discussionsupporting

confidence: 49%

Photocontrol of thylakoid protein synthesis in Euglena: differential post-transcriptional regulation depending on nutritional conditions

Weiss

Houlné

Schantz

1992

Planta

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“…Estos blanqueamientos también se han observado en colonias de Dunaliella salina en placas petri. Las posibles explicaciones incluyen la variación repentina en la concentración de oxigeno y/o variación de temperaturas (Ortiz, 1990). Cuando un alga como Euglena gracilis se expone a temperaturas superiores a 33 ºC inmediatamente presenta un aumento repentino de la concentración de la clorofila, que tiene su pico 15 horas después de la variación repentina de temperatura.…”

Section: Discussionunclassified

EFECTO DE DIFERENTES FUENTES DE NITRÓGENO EN EL CONTENIDO DE CAROTENOIDES Y CLOROFILA DE CUATRO CEPAS PERUANAS DE Dunaliella salina Teod.

Ibáñez

Rodríguez

2006

Ecol. apl.

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Resumen Se estudió el efecto de diferentes fuentes de nitrógeno (NO 3 NH 4 , SO 4 (NH 4) 2 , NO 3 K , NO 3 Na y Urea) en el crecimiento y contenido de pigmentos carotenoides y clorofila en cuatro cepas peruanas de Dunaliella salina. A los 27 días, el sulfato de amonio (y después el nitrato de amonio) produjeron la más alta densidad celular y contenido de pigmentos (mg/l) en las siguientes cepas: FV-002 (Salina de Huacho) y FV-003 (Salina de Chilca). Sin embargo, en los tratamientos con sulfato de amonio, se observó el blanqueamiento de los cultivos algunos días después.

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“…The initial stage of this process is characterized by selective protein breakdown, especially of Rubisco large and small subunits, which are almost undetectable after 33 h of treatment (Ortiz and Wilson 1988). The synthesis of the chloroplast-encoded large subunit of Rubisco ceases shortly after exposure to restrictive temperatures, whereas some nuclear-encoded chloroplast proteins of unidentified function are induced (Ortiz 1990).…”

mentioning

confidence: 99%

CORRELATED BIOCHEMICAL AND ULTRASTRUCTURAL CHANGES IN NITROGEN‐STARVED EUGLENA GRACILIS¹

García-Ferris

Rı́os

Ascaso

et al. 1996

Journal of Phycology

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Growth of Euglena gracilis Z Pringsheim under photoheterotrophic conditions in a nitrogen‐deprived medium resulted in progressive loss of chloroplastic material until total bleaching of the cells occurred. Biochemical analysis and ultrastructural observation of the first stages of the starvation process demonstrated an early lag phase (from 0 to 9 h) in which cells increased in size, followed by a period of cell division, apparently supported by the mobilization of some chloroplastic proteins such as the photosynthetic CO2‐fixing enzyme ribulose‐1,5‐bisphosphate carboxylase/oxygenase. The degradation of the enzyme started after 9 h of starvation and was preceded by a transient concentration of this protein in pyrenoidal structures. Protein nitrogen and photosynthetic pigments as well as number of chloroplasts per cell decreased during proliferation through mere distribution among daughter cells. However, after 24 h, when cell division had almost ceased, there was a slow but steady decline of photosynthetic pigments. This was paralleled by observable ultrastructural changes including progressive loss of chloroplast structure and accumulation of paramylon granules and lipid globules in the cytoplasm. These findings reinforce the role of chloroplastic materials as a nitrogen source during starvation of E. gracilis in a carbon‐rich medium. The excess of ribulose‐1,5‐bisphosphate carboxylase/oxygenase acts as a first reservoir that, once exhausted, is superseded by the generalized disassembly of the photosynthetic structures, if the adverse environment persists more than 24 h.

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Protein Synthesis during the Initial Phase of the Temperature-Induced Bleaching Response in Euglena gracilis

Abstract: ABSTRACT

Cited by 7 publications

References 17 publications

Photocontrol of thylakoid protein synthesis in Euglena: differential post-transcriptional regulation depending on nutritional conditions

Photocontrol of thylakoid protein synthesis in Euglena: differential post-transcriptional regulation depending on nutritional conditions

EFECTO DE DIFERENTES FUENTES DE NITRÓGENO EN EL CONTENIDO DE CAROTENOIDES Y CLOROFILA DE CUATRO CEPAS PERUANAS DE Dunaliella salina Teod.

CORRELATED BIOCHEMICAL AND ULTRASTRUCTURAL CHANGES IN NITROGEN‐STARVED EUGLENA GRACILIS¹

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Protein Synthesis during the Initial Phase of the Temperature-Induced Bleaching Response in Euglena gracilis

Abstract: ABSTRACT

Cited by 7 publications

References 17 publications

Photocontrol of thylakoid protein synthesis in Euglena: differential post-transcriptional regulation depending on nutritional conditions

Photocontrol of thylakoid protein synthesis in Euglena: differential post-transcriptional regulation depending on nutritional conditions

EFECTO DE DIFERENTES FUENTES DE NITRÓGENO EN EL CONTENIDO DE CAROTENOIDES Y CLOROFILA DE CUATRO CEPAS PERUANAS DE Dunaliella salina Teod.

CORRELATED BIOCHEMICAL AND ULTRASTRUCTURAL CHANGES IN NITROGEN‐STARVED EUGLENA GRACILIS1

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CORRELATED BIOCHEMICAL AND ULTRASTRUCTURAL CHANGES IN NITROGEN‐STARVED EUGLENA GRACILIS¹