Protein synthesis measurement in cancer patients with 13C valine

Egerland, A.; Reynier, J; Ballèvre, Olivier; Di-Costanzo, J; Obled, Christiane; Arnal, M.

doi:10.1016/0009-8981(96)06313-9

Cited by 6 publications

(4 citation statements)

References 13 publications

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“…In the present experiment the flooding dose technique was used, which involves the injection of a relatively large amount of unlabeled AA together with the tracer AA to minimize differences in isotopic enrichment of the free AA in plasma and tissue compartments (Garlick et al, 1989). This technique is particularly well suited for the study of tissues with rapid protein turnover and associated cell renewal (Egerland et al, 1996), as is expected to be the case in neonatal animals. In contrast to the constant infusion technique, improved detection of precursor enrichment and shorter periods of measurement are additional advantages of this method (Rathmacher, 2000).…”

Section: Tracer Studiesmentioning

confidence: 99%

“…Valine was chosen as a carrier of the tracer based on metabolic and physiochemical criteria. Valine has several advantages compared with other AA used in other tracer studies: 1) It is safe because even high amounts are nontoxic (Egerland et al, 1996); 2) it is relatively inexpensive (Sève et al, 1993); 3) it is the most soluble essential AA; 4) it can easily be purified by chromatography; 5) it does not stimulate insulin secretion, which is a well-known factor that increases PS (Egerland et al, 1996); and 6) the Val content in proteins is generally high (Egerland et al, 1996). Hence, relatively high PS rates can be expected, thus allowing a relatively sensitive measurement system to be established.…”

Section: Tracer Studiesmentioning

confidence: 99%

“…Hence, relatively high PS rates can be expected, thus allowing a relatively sensitive measurement system to be established. However, the tracer influences the K s value because the PS rate depends on the incorporation rate of an AA and not on all steps that finally result in protein deposition (Egerland et al, 1996). Therefore, the synthesis rate differs depending on which AA is used.…”

Section: Tracer Studiesmentioning

confidence: 99%

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Protein Synthesis in Jejunum and Liver of Neonatal Calves Fed Vitamin A and Lactoferrin

Rufibach

Stefanoni

Rey-Roethlisberger

et al. 2006

Journal of Dairy Science

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Rates of protein synthesis (PS) and turnover are more rapid during the neonatal period than during any other stage of postnatal life. Vitamin A and lactoferrin (Lf) can stimulate PS in neonates. However, newborn calves are vitamin A deficient and have a low Lf status, but plasma vitamin A and Lf levels increase rapidly after ingestion of colostrum. Neonatal calves (n = 6 per group) were fed colostrum or a milk-based formula without or with vitamin A, Lf, or vitamin A plus Lf to study PS in the jejunum and liver. l-[(13)C]Valine was intravenously administered to determine isotopic enrichment of free (nonprotein-bound) Val (AP(Free)) in the protein precursor pool, atom percentage excess (APE) of protein-bound Val, fractional protein synthesis rate (FSR) in the jejunum and liver, and isotopic enrichment of Val in plasma (APE(Pla)) and in the CO(2) of exhaled air (APE(Ex)). The APE, AP(Free), and FSR in the jejunum and liver did not differ significantly among groups. The APE(Ex) increased, whereas APE(Pla) decreased over time, but there were no group differences. Correlations were calculated between FSR(Jej) and histomorphometrical and histochemical data of the jejunum, and between FSR(Liv) and blood metabolites. There were negative correlations between FSR(Liv) and plasma albumin concentrations and between FSR(Jej) and the ratio of villus height:crypt depth, and there was a positive correlation between FSR(Jej) and small intestinal cell proliferation in crypts. Hence, there were no effects of vitamin A and Lf and no interactions between vitamin A and Lf on intestinal and hepatic PS. However, FSR(Jej) was correlated with histomorphometrical traits of the jejunum and FSR(Liv) was correlated with plasma albumin concentrations.

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Section: Tracer Studiesmentioning

confidence: 99%

Section: Tracer Studiesmentioning

confidence: 99%

Section: Tracer Studiesmentioning

confidence: 99%

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Protein Synthesis in Jejunum and Liver of Neonatal Calves Fed Vitamin A and Lactoferrin

Rufibach

Stefanoni

Rey-Roethlisberger

et al. 2006

Journal of Dairy Science

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“…Phenylalanine is the most widely used AA for the flooding dose method. Valine is also used, although less frequently, to measure tissue protein synthesis in a number of different species, such as sheep (Attaix et al., ), pigs (Sève et al., ; Hamard et al., ), humans (Egerland et al., ) and rats (Pirman et al., ). The use of valine, rather than phenylalanine, is a more sensible approach when examining synthesis of mucosal proteins, especially mucin proteins, which have low phenylalanine content (Faure et al., ).…”

Section: Introductionmentioning

confidence: 99%

Influence of a flooding dose of valine on key indicators of metabolic status in the growing pig

Libao-Mercado

Columbus

Lange

2014

Animal Physiology Nutrition

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A key concern with the flooding dose technique for measuring protein synthesis is that a large dose of amino acid (AA) can potentially change the animals' hormonal and nutritional status, which in turn can influence protein synthesis. Among stable isotope tracers, 1-[(13)C]-valine is the preferred AA for measuring protein synthesis in gut tissue and mucins. A study was conducted to determine the impact of a flooding dose of valine on the metabolic status of pigs. Six barrows [16.5 kg body weight (BW)] were randomly assigned to intravenous infusions of either 150 mM valine (1.5 mmol/kg BW) or physiological saline, following a crossover design. Blood samples were taken 10 min prior to infusion, at the end of infusion, at 10-min intervals for 60 min post-infusion, and at 90 and 120 min post-infusion. Plasma concentrations of insulin, glucose, AA, urea nitrogen and packed cell volume (PCV) were measured. Infusion of valine increased plasma valine concentrations (4129 vs. 582 μM; P < 0.05) but had no influence on PCV (26.4% vs. 27.2%) and plasma concentrations of glucose (6.0 vs. 5.8 mM) and insulin (8.2 vs. 8.5 μU/ml; P > 0.10). Plasma urea nitrogen concentration was reduced with valine infusion (8.5 vs. 7.8 mg/dl; P < 0.05). A flooding dose of valine had no impact on plasma concentrations of AA, and specifically branched-chain AA such as leucine (240 vs. 231 μM) and isoleucine (310 vs. 331 μM; P > 0.10). There was, however, a slight increase in the plasma concentrations of threonine (224 vs. 263 μM; P < 0.05) and a tendency towards reduced glycine (1387 vs. 1313 μM; P < 0.10). The results indicate that a flooding dose of valine does not cause a substantial change in the metabolic status of growing pigs and is therefore suitable for measuring protein synthesis rates.

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Determination of δ13C values of valine in protein hydrolysate by gas chromatography–combustion isotope ratio mass spectrometry

Kulik

Meesterburrie

Jakobs

et al. 1998

Journal of Chromatography B: Biomedical Sciences and Applicatio

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Protein synthesis measurement in cancer patients with 13C valine

Cited by 6 publications

References 13 publications

Protein Synthesis in Jejunum and Liver of Neonatal Calves Fed Vitamin A and Lactoferrin

Protein Synthesis in Jejunum and Liver of Neonatal Calves Fed Vitamin A and Lactoferrin

Influence of a flooding dose of valine on key indicators of metabolic status in the growing pig

Determination of δ13C values of valine in protein hydrolysate by gas chromatography–combustion isotope ratio mass spectrometry

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