Protein synthesis patterns in conjugating Tetrahymena thermophila

Ron, A.; Suhr-Jessen, Peter

doi:10.1016/0014-4827(81)90324-4

Cited by 18 publications

(5 citation statements)

References 26 publications

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“…The 35S-methionine labeling of cellular proteins has been used in a number of studies of the physiology and development of Tezrahyrnena. to detect shifts in protein synthesis patterns during heat shock (6,17), conjugation (8, 16), and various starvation conditions (Van Bell, unpublished). The 12SI-labeling of surface membrane proteins has been used to reveal both species-and strain-specific differences within the genus Tetrahyrnena (20) and has identified physiologically and developmentally mediated changes in membrane protein expression in T. thermophilu (Van Bell, manuscript in preparation).…”

Section: Discussionmentioning

confidence: 99%

Biochemical Approaches to Mating Type Expression in Tetrahymena thermophila¹

Bell

Williams

1983

The Journal of Protozoology

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Two‐dimensional gel electrophoresis was used to identify the patterns of protein synthesis during initiation, and the patterns of membrane protein expression following initiation, in all of the mating types of the Tetrahymena thermophila B family. In addition, one‐dimensional analysis was used to survey 125I‐Concanavalin A‐binding proteins. Although a large number of proteins was identified by each technique, no variation among the mating types was observed.

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Section: Discussionmentioning

confidence: 99%

Biochemical Approaches to Mating Type Expression in Tetrahymena thermophila¹

Bell

Williams

1983

The Journal of Protozoology

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“…Various stains have been used following fixation. The most frequent appear to be aceto‐carmine (Ray 1956a, 1956b), acid fuchsin (Napolitano, Wall, and Ganz 1970), Feulgen reaction (Chen, Luo, and Cao 1982; Ron and Suhr‐Jessen 1981; Wee 1983), Giemsa (Culbertson 1981), hematoxylin (Ron and Suhr‐Jessen 1981; Willumsen 1982), Kernechtrot (Willumsen, Siemensma, and Suhr‐Jessen 1987), methyl green and pyronin (Kovalchuk 1980), NMF (Borror 1969), and protargol method (Borror 1962, 1966, 1968b; Read et al 1983; Shyam and Sarma 1975). With regard to the Feulgen reaction, Golikova et al (1980) studied the intensity of the reaction in macro‐ and micronuclei of Paramecium bursaria under various conditions of hydrolysis and age of the organism.…”

Section: Epiloguementioning

confidence: 99%

“…The Fixative was used in studies of conjugation in the ciliates Paramecium polycaryum (Diller 1958), Tetrahymena thermophilia (Ron and Suhr‐Jessen 1981; Weiske‐Benner and Eckert 1985) and Aspidisca costata (Diller 1975), and in studying mitosis in Gonium pectorale (Shyam and Sarma 1975). In regard to algal flagellates, Shyam (1978) described a double fixation method using the Fixative and Carnoy's Fluid, followed by aceto‐carmine stain.…”

Section: Epiloguementioning

confidence: 99%

Behind the Discovery of “Nissenbaum's Fixative”

Nissenbaum¹

2001

J Eukaryotic Microbiology

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The author describes the serendipitous discovery, conception, development, and history of Nissenbaum's Fixative while an undergraduate biology major in the early 1950s. The subsequent uses, applications, and modifications over the past forty-seven years are also described. Some of the modifications omitted from his short original paper are mentioned. Highlights of his subsequent career in the field of medicine are noted.

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“…Other pre-pairing processes include a cessation of food vacuole formation (Frisch and Loyter, 1977;Casci and Hufnagle, 1988); a transient increase in the synthesis of p80, and 80 kD polypeptide (Garfinkel and Wolfe, 1981;Ron and Suhr-Jessen, 1981;Van Bell, 1983;Suhr-Jessen et al, 1986); and the synthesis of some required RNA (Allewell, 1976;Allewell and Wolfe, 1977).…”

Section: Introductionmentioning

confidence: 99%

Early encounters of the repetitive kind: A prelude to cell adhesion in conjugating Tetrahymena thermophila

Brown

Tirone

Wolfe

1993

Developmental Dynamics

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The relationship between direct cell contacts and subsequent cell-cell adhesion was studied in the ciliated protozoan, Tetruhymenu thermophila. During sexual reproduction, adhesion into pairs begins at approximately 1 hr after mixing starved complementary mating types. However, direct contacts between cells prior to pairing are known to be required for the development of adhesion-readiness. We find here that the initial contact interactions are necessary but not sufficient to drive the cells to adhesion-readiness. Secondary interactions are needed.Two distinct experimental strategies were used. First, we examined the effects of a mutant that is unable to pair but which can stimulate two different wild-type mating type cells to pair when mixed. We showed that stimulation by the mutant is only partial; in response to mutant cells, wildtype cells ceased forming food vacuoles but did not undergo tip transformation or concanavalin A (Con A)-receptor tipping. Further, kinetic analysis shows that when mixed together, pair-formation among partially stimulated wild-type cells is slightly delayed, allowing time for these pre-pairing processes to occur. This indicates that, beyond the initial contact interaction, mutant-stimulated wild-type cells require a subsequent interaction which cannot be fulfilled by the mutants. Secondly, we found that by blocking contact interactions between wild-type mating types at various time intervals after they were mixed, additional increase in tip transformation and Con A receptor tipping was prevented. Further, both processes underwent a regression. This indicates that multiple contact interactions are required to drive the cells to adhesion readiness and to prevent developmental slip-back. o 1993 Wiley-Liss, Inc.

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Protein synthesis patterns in conjugating Tetrahymena thermophila

Cited by 18 publications

References 26 publications

Biochemical Approaches to Mating Type Expression in Tetrahymena thermophila¹

Biochemical Approaches to Mating Type Expression in Tetrahymena thermophila¹

Behind the Discovery of “Nissenbaum's Fixative”

Early encounters of the repetitive kind: A prelude to cell adhesion in conjugating Tetrahymena thermophila

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Protein synthesis patterns in conjugating Tetrahymena thermophila

Cited by 18 publications

References 26 publications

Biochemical Approaches to Mating Type Expression in Tetrahymena thermophila1

Biochemical Approaches to Mating Type Expression in Tetrahymena thermophila1

Behind the Discovery of “Nissenbaum's Fixative”

Early encounters of the repetitive kind: A prelude to cell adhesion in conjugating Tetrahymena thermophila

Contact Info

Product

Resources

About

Biochemical Approaches to Mating Type Expression in Tetrahymena thermophila¹

Biochemical Approaches to Mating Type Expression in Tetrahymena thermophila¹