Protein-templated gold nanoclusters as fluorescence probes for the detection of methotrexate

Abstract

In this contribution, bovine serum albumin stabilized gold nanoclusters as novel fluorescent probes were successfully utilized for the detection of methotrexate for the first time. Our prepared gold nanoclusters exhibited strong emission with peak maximum at 633.5 nm. However, the addition of methotrexate induced the strong fluorescence intensity of the gold nanoclusters to decrease. The decrease in fluorescence intensity of the gold nanoclusters caused by methotrexate allowed the sensitive detection of methotrexate in the range of 0.0016 μg mL(-1) to 24 μg mL(-1). The detection limit for methotrexate is 0.9 ng mL(-1) at a signal-to-noise ratio of 3. The present sensor for methotrexate detection possessed a low detection limit and wide linear range. In addition, the real samples were analyzed with satisfactory results.

A dual-responsive fluorescence method for the detection of clenbuterol based on BSA-protected gold nanoclusters

“…The BSA-protected AuNCs (AuNCs@BSA) have been used to detect hydrogen peroxide (H 2 O 2 ) [27,28], metal ions [29][30][31][32], small biomolecules as trypsin [33], xanthine [34] and glucose [35], anions as cyanide [36], medicament as methotrexate [37] and so on. The good performance of AuNCs@BSA in these areas illustrated it was an excellent fluorescence probe for the analysis of specific target.…”

Naked-eye nanobiosensor for therapeutic drug monitoring of methotrexate

“…Other strategies that have been proposed for MTX sensing include methods based on electrochemistry, [16][17][18][19][20][21] nanoparticlebased optical detection [22][23][24] or bioluminescence. 25 We recently introduced a colorimetric test for MTX based on a competitive assay involving folic acid-derived Au NP and human dihydrofolate reductase (hDHFR).…”

Fluorescent Gold Nanoclusters: Promising Fluorescent Probes for Sensors and Bioimaging

“…Moreover, other important small biomolecules, such as ATP [79,92], bilirubin [93], cholesterol [94], FA [95], heparin [96], histidine [97], methotrexate [98], urea [99], and uric acid [100], can also be detected by using fluorescent AuNCs.…”

“…The fluorescent BSA-AuNCs have been used for the rapid detection of drug molecules including vitamin B12 [120], methotrexate [121], and organophosphorus pesticides (OPs) [60]. For example, an efficient fluorimetric sensing platform was constructed for sensitive detection of OPs by tyrosinase (TYR) enzyme-controlled quenching of AuNCs [60].…”