Protein tethered lipid bilayer: An alternative mimic of the biological membrane (Mini Review)

Naumann, Renate; Knoll, Wolfgang

doi:10.1116/1.2936939

Cited by 21 publications

(12 citation statements)

References 33 publications

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“…The phospholipid bilayer is an important component of cell membranes that regulates material transport in a wide range of conditions [1][2][3]. It was first extracted and identified by Gorter and Grendel in 1925 [4].…”

Section: Introductionmentioning

confidence: 99%

Surface energy and separation mechanics of droplet interface phospholipid bilayers

Huang

Suja

Tajuelo

et al. 2021

J. R. Soc. Interface.

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Droplet interface bilayers are a convenient model system to study the physio-chemical properties of phospholipid bilayers, the major component of the cell membrane. The mechanical response of these bilayers to various external mechanical stimuli is an active area of research because of its implications for cellular viability and the development of artificial cells. In this article, we characterize the separation mechanics of droplet interface bilayers under step strain using a combination of experiments and numerical modelling. Initially, we show that the bilayer surface energy can be obtained using principles of energy conservation. Subsequently, we subject the system to a step strain by separating the drops in a step-wise manner, and track the evolution of the bilayer contact angle and radius. The relaxation time of the bilayer contact angle and radius along with the decay magnitude of the bilayer radius were observed to increase with each separation step. By analysing the forces acting on the bilayer and the rate of separation, we show that the bilayer separates primarily through the peeling process with the dominant resistance to separation coming from viscous dissipation associated with corner flows. Finally, we explain the intrinsic features of the observed bilayer separation by means of a mathematical model comprising the Young–Laplace equation and an evolution equation. We believe that the reported experimental and numerical results extend the scientific understanding of lipid bilayer mechanics, and that the developed experimental and numerical tools offer a convenient platform to study the mechanics of other types of bilayers.

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Section: Introductionmentioning

confidence: 99%

Surface energy and separation mechanics of droplet interface phospholipid bilayers

Huang

Suja

Tajuelo

et al. 2021

J. R. Soc. Interface.

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show abstract

“…Strong evidence that the electron transfer observed by cyclic voltammetry takes place directly within the enzyme was derived from Soret band excited surface-enhanced resonance Raman spectroscopy (SERRS) taken as a function of potential. 240,241 The cyclic voltammogram of COX oriented with the cytochrome c binding side directed toward the electrode was used to determine the functional activity of the enzyme as a function of its surface density. 242 This density was varied by diluting the thiol functionalized with the chelator nitrilotriacetic (NTA) moiety with a nonfunctionalized thiol that did not bind to the enzyme.…”

Section: Protein-tethered Bilayer Lipid Membranes (Ptblms)mentioning

confidence: 99%

4 Electrochemistry of Biomimetic Membranes

Guidelli

Becucci

2011

Modern Aspects of Electrochemistry

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“…The phospholipid bilayer is an important component of cell membrane that regulates material transport in a wide range of conditions [1][2][3]. It was first extracted and identified by Gorter and Grendel in 1925 [4].…”

Section: Introductionmentioning

confidence: 99%

Surface Energy and Separation Mechanics of Droplet Interface Phospholipid Bilayers

Huang,

Suja,

Tajuelo

et al. 2020

Preprint

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Droplet interface bilayers are a convenient model system to study the physio-chemical properties of phospholipid bilayers, the major component of the cell membrane. The mechanical response of these bilayers to various external mechanical stimuli is an active area of research due to implications for cellular viability and development of artificial cells. In this manuscript we characterize the separation mechanics of droplet interface bilayers under step strain using a combination of experiments and numerical modeling. Initially, we show that the bilayer surface energy can be obtained using principles of energy conservation. Subsequently, we subject the system to a step strain by separating the drops in a step wise manner, and track the evolution of the bilayer contact angle and radius. The relaxation time of the bilayer contact angle and radius, along with the decay magnitude of the bilayer radius were observed to increase with each separation step. By analyzing the forces acting on the bilayer and the rate of separation, we show that the bilayer separates primarily through the peeling process with the dominant resistance to separation coming from viscous dissipation associated with corner flows. Finally, we explain the intrinsic features of the observed bilayer separation by means of a mathematical model comprising of the Young-Laplace equation and an evolution equation. We believe that the reported experimental and numerical results extend the scientific understanding of lipid bilayer mechanics, and that the developed experimental and numerical tools offer a convenient platform to study the mechanics of other types of bilayers.

show abstract

Protein tethered lipid bilayer: An alternative mimic of the biological membrane (Mini Review)

Cited by 21 publications

References 33 publications

Surface energy and separation mechanics of droplet interface phospholipid bilayers

Surface energy and separation mechanics of droplet interface phospholipid bilayers

4 Electrochemistry of Biomimetic Membranes

Surface Energy and Separation Mechanics of Droplet Interface Phospholipid Bilayers

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