Protein tyrosine kinase activity in laryngeal squamous cell carcinoma

Verschuur, H. P.; Rijksen, Gert; Schipper-Kester, G. P. M.; Slootweg, Pieter J.; Hordijk, G. J.; Staal, Gerard E.J.

doi:10.1007/bf00168856

Cited by 12 publications

(2 citation statements)

References 13 publications

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“…These observations are in line with the concept of field cancerization proposed for HNSCC and are in accordance with previous studies describing increased PTK activity in normal mucosa of tumor patients compared with the mucosa of healthy patients. 53 Together with the high percentage (80%) of HNSCC with strong pSFK score the increased SFK activity in "normal" mucosa also indicates that activation of SFK is an early event in HNSCC development.…”

Section: Targeting Of Sfk In Hnscc Cellsmentioning

confidence: 98%

Analyzing tyrosine kinase activity in head and neck cancer by functional kinomics: Identification of hyperactivated Src family kinases as prognostic markers and potential targets

Bußmann

Hoffer

Bargen

et al. 2021

Intl Journal of Cancer

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Signal transduction via protein kinases is of central importance in cancer biology and treatment. However, the clinical success of kinase inhibitors is often hampered by a lack of robust predictive biomarkers, which is also caused by the discrepancy between kinase expression and activity. Therefore, there is a need for functional tests to identify aberrantly activated kinases in individual patients. Here we present a systematic analysis of the tyrosine kinases in head and neck cancer using such a testfunctional kinome profiling. We detected increased tyrosine kinase activity in tumors compared with their corresponding normal tissue. Moreover, we identified members of the family of Src kinases (Src family kinases [SFK]) to be aberrantly activated in the majority of the tumors, which was confirmed by additional methods. We could also show that SFK hyperphosphorylation is associated with poor prognosis, while inhibition of SFK impaired cell proliferation, especially in cells with hyperactive SFK. In summary, functional kinome profiling identified SFK to be frequently hyperactivated in head and neck squamous cell carcinoma. SFK may therefore be potential therapeutic targets. These results furthermore demonstrate how functional tests help to increase our understanding of cancer biology and support the expansion of precision oncology.

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Section: Targeting Of Sfk In Hnscc Cellsmentioning

confidence: 98%

Analyzing tyrosine kinase activity in head and neck cancer by functional kinomics: Identification of hyperactivated Src family kinases as prognostic markers and potential targets

Bußmann

Hoffer

Bargen

et al. 2021

Intl Journal of Cancer

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show abstract

“…6,7 Field cancerization is supported by clinical, histopathological, and recent molecular evidence. 1 In histologically normal tumour-adjacent mucosa (TAM), alterations were found, among others, in proliferation, 8,9 the extent of cytoplasmic areas, 10 the genetic state and expression of the tumour suppressor p53, 11,12 epidermal growth factor receptor expression, 13 protein tyrosine kinase activity, 14 and cytokeratin expression. 15 Some of these changes appeared to relate to the use of tobacco, such as increased proliferation 9 and expression of cytokeratin 8/cytokeratin 7.…”

Section: Introductionmentioning

confidence: 99%

p53 overexpression in oral mucosa in relation to smoking

Oijen¹,

Craats²,

Slootweg³

1999

J. Pathol.

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The tumour‐suppressor protein p53 is mutated in many head and neck squamous cell carcinomas (HNSCCs). In this immunohistochemical study, similar numbers of p53‐overexpressing cells were uniformly distributed throughout normal oral epithelium, irrespective of different smoking habits or the presence of an adjacent HNSCC. In a previous study, an increased number of proliferating cells were observed in normal oral mucosa from (ex)‐smoking individuals and the present observations indicate that overexpression of p53 does not play a role in this increase. In contrast, focally overexpressed p53 occurred more frequently (p < 0·05) in the tumour‐adjacent normal mucosa (TAM) from smoking HNSCC patients (50 per cent) than in that from non‐smoking HNSCC patients (20 per cent). This increase in focal p53 overexpression might represent an early alteration in the development of HNSCC, but it could not be detected in mucosa from healthy smokers. This indicates that besides the abuse of tobacco, other environmental and/or genetic factors must contribute to the presence of p53‐positive clusters in TAM. Abuse of alcohol, an additional factor in these HNSCC patients, together with the abuse of tobacco, might play a role in the development of the p53‐positive clusters. Copyright © 1999 John Wiley & Sons, Ltd.

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Larynx

Burkhardt¹,

Meyer-Breiting²

1999

Spezielle Pathologische Anatomie

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Protein tyrosine kinase activity in laryngeal squamous cell carcinoma

Cited by 12 publications

References 13 publications

Analyzing tyrosine kinase activity in head and neck cancer by functional kinomics: Identification of hyperactivated Src family kinases as prognostic markers and potential targets

Analyzing tyrosine kinase activity in head and neck cancer by functional kinomics: Identification of hyperactivated Src family kinases as prognostic markers and potential targets

p53 overexpression in oral mucosa in relation to smoking

Larynx

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