Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models

Blanco-Fernandez, Bárbara; Gaspar, Vítor M.; Engel, Elisabeth; Mano, João F.

doi:10.1002/advs.202003129

Cited by 51 publications

(53 citation statements)

References 290 publications

(357 reference statements)

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“…The tumor microenvironment (TME) modulates tumor progression and treatment efficacy (Blanco-Fernandez et al, 2021). Therefore, we evaluated the relationship between the angiogenesis score and the TME.…”

Section: High Angiogenesis Scores Are Associated With Hot Tumor Microenvironmentsmentioning

confidence: 99%

Association of Angiogenesis Gene Expression With Cancer Prognosis and Immunotherapy Efficacy

et al. 2022

Front. Cell Dev. Biol.

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Background: Several new blood vessels are formed during the process of tumor development. These new blood vessels provide nutrients and water for tumour growth, while spreading tumour cells to distant areas and forming new metastases in different parts of the body. The available evidence suggests that tumour angiogenesis is closely associated with the tumour microenvironment and is regulated by a variety of pro-angiogenic factors and/or angiogenic inhibitors.Methods: In the present study, a comprehensive characterization of angiogenesis genes expression was performed in a pan-cancer analysis across the 33 human cancer types. Further, genetic data from several public databases were also used in the current study. An angiogenesis score was assigned to The Cancer Genome Atlas (TCGA) pan-cancer data, with one angiogenesis score as per sample for each tumour.Results: It was found that angiogenesis genes vary across cancer types, and are associated with a number of genomic and immunological features. Further, it was noted that macrophages and iTreg infiltration were generally higher in tumours with high angiogenesis scores, whereas lymphocytes and B cells showed the opposite trend. Notably, NK cells showed significantly different correlations among cancer types. Furthermore, results of the present study showed that a high angiogenesis score was associated with poor survival and aggressive types of cancer in most of the cancer types.Conclusion: In conclusion, the current study evidently showed that the expression of angiogenesis genes is a key feature of tumour biology that has a major impact on prognosis of patient with cancers.

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Section: High Angiogenesis Scores Are Associated With Hot Tumor Microenvironmentsmentioning

confidence: 99%

Association of Angiogenesis Gene Expression With Cancer Prognosis and Immunotherapy Efficacy

et al. 2022

Front. Cell Dev. Biol.

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“…Increased ECM stiffness is typically linked to tumour aggressiveness [31]. The recognition of the importance of the ECM in regulating developmental and oncogenic processes prompted research on biomaterials that can mimic the properties and dynamics of the ECM and development of scaffold-based 3D models [32][33][34][35].…”

Section: Introductionmentioning

confidence: 99%

3D Cancer Models: Depicting Cellular Crosstalk within the Tumour Microenvironment

Franchi-Mendes

Eduardo

Domenici

et al. 2021

Cancers

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The tumour microenvironment plays a critical role in tumour progression and drug resistance processes. Non-malignant cell players, such as fibroblasts, endothelial cells, immune cells and others, interact with each other and with the tumour cells, shaping the disease. Though the role of each cell type and cell communication mechanisms have been progressively studied, the complexity of this cellular network and its role in disease mechanism and therapeutic response are still being unveiled. Animal models have been mainly used, as they can represent systemic interactions and conditions, though they face recognized limitations in translational potential due to interspecies differences. In vitro 3D cancer models can surpass these limitations, by incorporating human cells, including patient-derived ones, and allowing a range of experimental designs with precise control of each tumour microenvironment element. We summarize the role of each tumour microenvironment component and review studies proposing 3D co-culture strategies of tumour cells and non-malignant cell components. Moreover, we discuss the potential of these modelling approaches to uncover potential therapeutic targets in the tumour microenvironment and assess therapeutic efficacy, current bottlenecks and perspectives.

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“…Its primary structure consists of a tetrapeptide, arginine–alanine–aspartate–alanine (RADA), resembling the tripeptide RGD, an amino acid sequence that promotes cell adhesion. Peptide self-assembly in different electrolytic solvents results in a β-sheet secondary structure, thanks to both ionic and hydrophobic interactions [ 249 ]. The self-assembly process is strongly related to peptide sequence, concentration, pH, and the presence of salts, leading to the formation of different structures, such as fibers, membranes, and hydrogels.…”

Section: Polymers For 3d Ovarian Cancer (Oc) Modelingmentioning

confidence: 99%

Polymeric Hydrogels for In Vitro 3D Ovarian Cancer Modeling

Braccini

Tacchini

Chiellini

et al. 2022

IJMS

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Ovarian cancer (OC) grows and interacts constantly with a complex microenvironment, in which immune cells, fibroblasts, blood vessels, signal molecules and the extracellular matrix (ECM) coexist. This heterogeneous environment provides structural and biochemical support to the surrounding cells and undergoes constant and dynamic remodeling that actively promotes tumor initiation, progression, and metastasis. Despite the fact that traditional 2D cell culture systems have led to relevant medical advances in cancer research, 3D cell culture models could open new possibilities for the development of an in vitro tumor microenvironment more closely reproducing that observed in vivo. The implementation of materials science and technology into cancer research has enabled significant progress in the study of cancer progression and drug screening, through the development of polymeric scaffold-based 3D models closely recapitulating the physiopathological features of native tumor tissue. This article provides an overview of state-of-the-art in vitro tumor models with a particular focus on 3D OC cell culture in pre-clinical studies. The most representative OC models described in the literature are presented with a focus on hydrogel-based scaffolds, which guarantee soft tissue-like physical properties as well as a suitable 3D microenvironment for cell growth. Hydrogel-forming polymers of either natural or synthetic origin investigated in this context are described by highlighting their source of extraction, physical-chemical properties, and application for 3D ovarian cancer cell culture.

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Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models

Cited by 51 publications

References 290 publications

Association of Angiogenesis Gene Expression With Cancer Prognosis and Immunotherapy Efficacy

Association of Angiogenesis Gene Expression With Cancer Prognosis and Immunotherapy Efficacy

3D Cancer Models: Depicting Cellular Crosstalk within the Tumour Microenvironment

Polymeric Hydrogels for In Vitro 3D Ovarian Cancer Modeling

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