Proteinase activity and stability of natural bromelain preparations

Hale, Laura P.; Greer, Paula K.; Trinh, Chau T.; James, Cindy L.

doi:10.1016/j.intimp.2004.12.007

Cited by 186 publications

(155 citation statements)

References 23 publications

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“…All biopsies were treated using aliquots derived from the same lot of bromelain. Bromelain proteolytic activity was monitored periodically using model substrate assays as described (14). The biopsies were washed with media + 10% FBS to remove and/or inactivate any residual bromelain, then cultured in 0.5 ml media.…”

Section: Culture Of Colon Biopsies and Cytokine Assaysmentioning

confidence: 99%

“…These proteinases have different substrate specificities toward both model substrates and cell surface molecules. The activity of individual proteinases within pharmaceutical bromelain preparations can be monitored using model substrate assays or by flow cytometric assessment of their effects on specific bromelain-sensitive cell surface molecules (14).…”

Section: Introductionmentioning

confidence: 99%

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Bromelain treatment decreases secretion of pro-inflammatory cytokines and chemokines by colon biopsies in vitro

Onken

Greer

Calingaert

et al. 2008

Clinical Immunology

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Oral bromelain has been anecdotally reported to decrease inflammation in ulcerative colitis (UC). Proteolytically active bromelain is known to decrease expression of mRNAs encoding proinflammatory cytokines by human leukocytes in vitro. To assess the effect of bromelain on mucosal secretion of cytokines in inflammatory bowel disease (IBD), endoscopic colon biopsies from patients with UC, Crohn's disease (CD), and non-IBD controls were treated in vitro with bromelain or media, then cultured. Secretion of pro-inflammatory cytokines and chemokines was measured. Significant increases in granulocyte colony stimulating factor (G-CSF), interferon (IFN)-γ, interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF) were detected in the media from actively inflamed areas in UC and CD as compared with non-inflamed IBD tissue and non-IBD controls. In vitro bromelain treatment decreased secretion of G-CSF, granulocyte-macrophage colony stimulating factor (GM-CSF), IFN-γ, CCL4/macrophage inhibitory protein (MIP)-1β, and TNF by inflamed tissue in IBD. Bromelain may be a novel therapy for IBD.

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Section: Culture Of Colon Biopsies and Cytokine Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bromelain treatment decreases secretion of pro-inflammatory cytokines and chemokines by colon biopsies in vitro

Onken

Greer

Calingaert

et al. 2008

Clinical Immunology

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“…These enzymes are a mixture of proteinases derived from pineapple stem, which is sold as a nutritional supplement to "promote digestive health" and as an anti-inflammatory medication in some developed countries (Hale et al, 2005a and2005b;Wen et al, 2006). Bromelain is clinically used from the pineapple extract and the natural product.…”

Section: Introduction Bromelain From Pineapple Steammentioning

confidence: 99%

“…Concentrated bromelain solutions are more resistant to spontaneous inactivation of their proteolytic activity than are dilute solutions. The relative stability of concentrated versus dilute bromelain solutions to inactivation under physiologically relevant conditions suggests that the delivery of bromelain as a concentrated bolus would be the preferred method to maximize its proteolytic activity in vivo (Hale et al, 2005b;Arroyo-Reyna and Hernández-Arana, 1995). Other problem in vivo studies using bromelain, is the limitation by the lack of assays to control for potential differences in the composition and proteolytic activity of this naturally derived proteinase mixture (Arroyo-Reyna and Hernández-Arana, 1995).…”

Section: Introduction Bromelain From Pineapple Steammentioning

confidence: 99%

The effect of pH on bromelain partition from Ananas comosus by PEG4000/phosphate ATPS

Ferreira

Santana

Tambourgi

2011

Braz. arch. biol. technol.

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“…Many publications have described the pharmacological activities displayed by various plant proteins (Hale et al, 2005, Oliveira et al, 2010, including those belonging to the Leguminoseae family (Leite et al, 2012). These plant proteins receive special attention, as they are promising molecules for human therapeutic treatment.…”

Section: Introductionmentioning

confidence: 99%

The anti-inflammatory and antinociceptive effects of proteins extracted from Acacia farnesiana seeds

Leal

Silva

Araújo

et al. 2016

Rev. bras. plantas med.

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Seeds of Acacia farnesiana are commonly sold in the local markets of northeastern Brazil as a therapeutic agent. The present work aimed to evaluate the anti-inflammatory and analgesic activities of proteins obtained from A. farnesiana seeds. Five different protein fractions (albumin, globulin, prolamin, acidic and basic glutelins) were obtained and investigated for the protein pattern, the presence of hemagglutinating and proteolytic activities. The globulin fraction (GLB) was also evaluated for anti-inflammatory and analgesic activities. Globulins reduced the paw edema induced by carrageenan in a dose-dependent manner, which was accompanied by a reduction of myeloperoxidase activity (p < 0.05). Additionally, GLB reduced the neutrophil peritoneal migration induced by carrageenan. However, GLB was not able to inhibit the edema triggered by dextran. Pre-treatment with globulins reduced the abdominal constrictions induced by acetic acid as well as the paw licking time induced by formalin (69.1% at first phase). However, it did not produce a significant antinociceptive effect in the hot plate test (55-56 °C). Treating the GLB with heat (at 100 °C for 30 min) abolished its anti-edematogenic and hemagglutinating activities. Our results showed that seeds from A. farnesiana are a source of proteins with antiinflammatory and analgesic properties.Keywords: cysteine proteinase, Coronha, inflammation, lectin, nociception RESUMO: Efeito antiiflamatório e antinociceptivo de proteínas extraídas de sementes de Acacia farnesiana. Sementes de Acacia farnesiana são comumente vendidas em feiras locais no nordeste do Brasil como agente terapêutico. O presente trabalho objetivou avaliar as atividades antiinflamatória e antinociceptiva de proteínas obtidas de sementes de A. farnesiana. Cinco frações protéicas distintas (albuminas, globulinas, prolaminas, glutelinas ácidas e básicas) foram obtidas e investigadas quanto o perfil de proteínas, presença de atividade hemaglutinante e proteolítica. A fração globulina (GLB) também foi avaliada quanto a presença de atividade antiinflamatória e analgésica. Globulinas reduziram o edema de pata induzido por carragenina de modo dependente da dose que foi acompanhada da redução da atividade da mieloperoxidase (p < 0,05). Em adição, GLB reduziu a migração de neutrófilos para cavidade peritoneal induzida por carragenina. Entretanto, GLB não foi capaz de inibir o edema induzido por dextrana. O pré-tratamento com globulinas reduziu as contorções abdominais induzidas por ácido acético, bem como o tempo de lambedura da pata induzida por formalina (69.1% na primeira fase). Por outro lado, GLB não produziu um efeito antinociceptivo significante no teste de placa quente (55-56 °C). O pré-tratamento de GLB com calor (100 °C por 30 min) aboliu sua atividade anti-edematogênica e hemaglutinante. Nossos resultados mostraram que sementes de A. farnesiana são fonte de proteínas com propriedades antiinflamatórias e analgésicas.

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Proteinase activity and stability of natural bromelain preparations

Cited by 186 publications

References 23 publications

Bromelain treatment decreases secretion of pro-inflammatory cytokines and chemokines by colon biopsies in vitro

Bromelain treatment decreases secretion of pro-inflammatory cytokines and chemokines by colon biopsies in vitro

The effect of pH on bromelain partition from Ananas comosus by PEG4000/phosphate ATPS

The anti-inflammatory and antinociceptive effects of proteins extracted from Acacia farnesiana seeds

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