Proteinase inhibition of the detection of Listeria monocytogenes in milk using the polymerase chain reaction

Powell, Hilary A.; Gooding, Christopher M.; Garrett, S. D.; Lund, Barbara M.; McKEE, R. K.

doi:10.1111/j.1472-765x.1994.tb00802.x

Cited by 137 publications

(76 citation statements)

References 10 publications

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“…This result is similar with other reports of the lack of extracted DNA from highly fermented products such as soy margarine, soy sauce, and soy and maize oil (Abdullah et al, 2006;Greiner et al, 2005). In addition, since milk products include factors such as proteinase that inhibits the PCR reaction (Powell et al, 1994;Rossen et al, 1992;Wernars et al, 1991), no amplification was presently apparent despite sufficient DNA concentration for PCR. Hence, other technical methods are needed for better efficiency of DNA extraction from fermented dairy products such as cheese.…”

Section: Not Testedsupporting

confidence: 80%

Validation of Korean Meat Products and Processed Cheese for the Detection of GMO using p35S and tNOS Primers

Shin¹,

Heo²,

Moon³

et al. 2011

Korean Journal for Food Science of Animal Resources

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In this study, 543 samples of press hams, sausages, processed ground meat and processed cheese acquired from retail markets in Seoul and Gyeonggi province in Korea from 2005 to 2010 were monitored using a one-step multiplex polymerase chain reaction (PCR) method that involves the amplification of specific soya or maize endogenous genes and the amplification of 35S promoter (p35S) and nopaline synthase terminator (tNOS) for GMO detection. Among the 543 samples, 477 samples were amplified for maize and/or soybean endogenous genes. Although one sausage sample collected in 2008 showed amplification of tNOS, the result was assumed to be false positive based on the results from further tests of other sausage samples of the same brand. Our results demonstrate the absence of GM soya and/or maze of livestock products in the Korean market during 2005-2010. In addition, the one-step multiplex PCR using previously constructed primer sets appears to be useful as a screening method for the detection of GMOs in processed livestock products. However, more specific methods should be established and employed to detect the event-specific GM gene for positive reaction samples by screening tests in processed livestock products.

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Section: Not Testedsupporting

confidence: 80%

Validation of Korean Meat Products and Processed Cheese for the Detection of GMO using p35S and tNOS Primers

Shin¹,

Heo²,

Moon³

et al. 2011

Korean Journal for Food Science of Animal Resources

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“…In theory, RNA detection is indicative of metabolically active organisms, but often requires an enrichment step prior to the molecular biological analysis (Blais et al, 1997 ;Klein & Juneja, 1997). This precludes simple, rapid quantification and suffers from poor sensitivity when attempted directly on food samples (Powell et al, 1994 ;Wang et al, 1992 ;Cano et al, 1995 ;Makino et al, 1995). (Kjellgaard & Kurland 1963 ;Rosset et al, 1966 ;Gausing, 1977 ;Kerkhof & Ward, 1993 ;Amann et al, 1990a, b ;Muttray & Mohn, 1998).…”

Section: Introductionmentioning

confidence: 99%

Relationship between nucleic acid ratios and growth in Listeria monocytogenes

2001

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Listeria monocytogenes is a pathogen whose distribution in a range of foodstuffs requires the development of methods for sensitive and rapid detection. Molecular biological methods usually rely on specific detection of L. monocytogenes rDNA directly amplified by the application of PCR to DNA extracts. Information on the metabolic status of L. monocytogenes populations would be valuable and can, in theory, be provided by quantitative detection of rRNA itself. Both fluorometry and oligonucleotide probe assays were applied to L. monocytogenes cultures to quantify RNA and DNA and produced more meaningful data than previous estimates for bacteria based on eukaryotic nucleic acid standards. In batch culture, the RNA-DNA ratio was found to be greatest at the end of exponential growth, after which RNA became degraded in accordance with the rapid decrease in viability. When the pH of the medium was controlled at neutrality, culture viability was dramatically extended and although RNA was degraded, intact DNA was maintained for the duration of the experiment. Ribosome numbers per cell were estimated to decrease from about 25 000 observed during mid-exponential growth to about 600 during stationary phase, under pH-controlled conditions. Like Escherichia coli, therefore, L. monocytogenes loses viability and rRNA rapidly once exponential growth has ceased in batch culture. However, much improved survival of a culturable L. monocytogenes population when pH is controlled has clear implications for the persistence of this species in buffered environments such as dairy products.

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“…However all mycoplasma species could be detected when mycoplasmas were collected from the simulated specimens by centrifugation and lysed with a mycoplasmal lysis buffer. Because a large number of milk components remain in template DNA in milk samples, these components may have interfered with the PCR reaction [2,16,18]. Therefore, we tried to eliminate milk components from the simulated specimens by centrifugation.…”

mentioning

confidence: 99%

Detection of Mycoplasma in Mastitic Milk by PCR Analysis and Culture Method.

Hirose

Kawasaki

Kotani

et al. 2001

The Journal of Veterinary Medical Science

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ABSTRACT. Mycoplasma alkalescens, M. bovigenitalium, M. bovirhinis and M. bovis were directly detected from milk specimens by a polymerase chain reaction (PCR) when milk specimens were centrifuged and treated with mycoplasmal lysis buffer. The sensitivity of this PCR method was 110 to 1,400 colony forming units (CFU). This method was useful for the detection of mycoplasmas in milk specimens from cows at an early stage of mycoplasmal mastitis since a small amount of mycoplasma could be detect in milk without culture. The results were available within 12 hr, which is faster than conventional culture techniques. M. bovirhinis was detected in more than 70% of mastitic milk specimens when mycoplasmas were detected in milk specimens from 30 cows with mastitis by this PCR method. KEY WORDS: mastitis, mycoplasma, PCR.J. Vet. Med. Sci. 63(6): 691-693, 2001 Mycoplasma mycoides subsp. mycoides, M. bovis, M. alkalescens, M. bovigenitalium, M. dispar and others are the causative agents of several diseases in cows and calves, such as mastitis, pneumonia, arthritis, genital disorders and abortions [1,3,6,12,15]. M. bovirhinis is a troublesome agent as a secondary invader in respiratory diseases, and it is the mycoplasma that is the most common isolate from the nasal cavity of cattle with respiratory disease [8]. Since the diseases are largely resistant to chemotherapy [6,24], it is necessary to have rapid and reliable diagnostic methods to detect the agents at an early stage, so that effective control measures can be introduced in time. However, current methods for the detection of mycoplasmas are restricted to culture and serology, and both of these diagnostic methods are time consuming, laborious and difficult. Isolation and identification of mycoplasmas may take weeks, and few laboratories have the capabilities required to routinely culture mycoplasmas. Serodiagnosis requires the demonstration of increasing antibody titers that are reached only ten to fourteen days after the onset of clinical symptoms [19]. Consequently, the pathogen cannot be detected during the incubation period. Moreover, the serological cross reactions among the mycoplasma species are a critical problem [19]. Therefore, the absence of practical and rapid detection methods and resistance to therapy hamper the effective control of mycoplasma infections.Gonzalez [7] and Hatanaka et al. [9] reported cases of mycoplasmal mastitis caused by M. bovis derived from the nasal secretion of cows with pneumonia, emphasizing the relationship between mycoplasmal pneumonia and mastitis. During 1996-1997, we performed nationwide research on the isolation of mycoplasma from the nasal secretions of calves with respiratory diseases, and the result was that a strain of mycoplasma was isolated from about 30% of calves with respiratory diseases. The majority of the isolates consisted of the following 4 species, M. alkalescens, M. bovigenitalium, M. bovirhinis and M. bovis (Data not shown). Therefore, we restrict discussion to these four species in this study.The purpose of...

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Proteinase inhibition of the detection of Listeria monocytogenes in milk using the polymerase chain reaction

Cited by 137 publications

References 10 publications

Validation of Korean Meat Products and Processed Cheese for the Detection of GMO using p35S and tNOS Primers

Validation of Korean Meat Products and Processed Cheese for the Detection of GMO using p35S and tNOS Primers

Relationship between nucleic acid ratios and growth in Listeria monocytogenes

Detection of Mycoplasma in Mastitic Milk by PCR Analysis and Culture Method.

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