Proteiniclasticum sediminis sp. nov., an obligate anaerobic bacterium isolated from anaerobic sludge

Liu, Junwei; Bao, Yixuan; Zhang, Xuan; Chen, Yinran; Chen, Shuaimin; Wu, Haiyan; He, Jun

doi:10.1007/s10482-021-01620-9

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…The morphology of strain C-1 was observed using a S-4800 scanning electron microscope (Hitachi, Japan). The physiological-biochemical characteristics of strain C-1 were tested according to the methods previously described . The identification method for the 16S rRNA gene of strain C-1 has been previously described. , The identified 16S rRNA gene sequence of strain C-1 was submitted to NCBI and assigned the accession number (OQ719618).…”

Section: Methodsmentioning

confidence: 99%

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Novel Bifunctional Amidase Catalyzing the Degradation of Propanil and Aryloxyphenoxypropionate Herbicides in Rhodococcus sp. C-1

Zhou,

Huang,

et al. 2024

J. Agric. Food Chem.

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Propanil residues can contaminate habitats where microbial degradation is predominant. In this study, an efficient propanil-degrading strain C-1 was isolated from paddy and identified as Rhodococcus sp. It can completely degrade 10 μg/L−150 mg/L propanil within 0.33−10 h via the hydrolysis of the amide bond, forming 3,4-dichloroaniline. A novel bifunctional amidase, PamC, was identified in strain C-1. PamC can catalyze the hydrolysis of the amide bond of propanil to produce 3,4-dichloroaniline as well as the hydrolysis of the ester bonds of aryloxyphenoxypropionate herbicides (APPHs, clodinafop-propargyl, cyhalofop-butyl, fenoxaprop-p-ethyl, fluazifop-p-butyl, haloxyfop-p-methyl, and quizalofop-p-ethyl) to form aryloxyphenoxypropionic acids. Molecular docking and site-directed mutagenesis confirmed that the catalytic triad Lys82-Ser157-Ser181 was the active center for PamC to hydrolyze propanil and cyhalofop-butyl. This study presents a novel bifunctional amidase with capabilities for both amide and ester bond hydrolysis and enhances our understanding of the molecular mechanisms underlying the degradation of propanil and APPHs.

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Section: Methodsmentioning

confidence: 99%

“…A phylogenetic tree was constructed using MEGA 11.0 . The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) between strain C-1 and other closely related typical strains were calculated using the OrthoANIu algorithm tool () and the genome-to-genome distance calculator (), respectively …”

Section: Methodsmentioning

confidence: 99%

Novel Bifunctional Amidase Catalyzing the Degradation of Propanil and Aryloxyphenoxypropionate Herbicides in Rhodococcus sp. C-1

Zhou,

Huang,

et al. 2024

J. Agric. Food Chem.

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“…Bacteria from the Acinetobacter genus are capable of degrading aromatic hydrocarbons, phenol, diesel, petroleum, and amino acid derivatives [48]. Environmental isolates of Proteiniclasticum are responsible for the degradation of herbicides including acetochlor, chloroacetamide, and organoarsenic animal drugs [49][50][51][52]. Shewanellae have demonstrated the ability to reduce radionuclides, cobalt, uranium, chromium, mercury, and arsenic, as well as halogenated organic compounds and nitramines [40].…”

Section: Biodiversity Of Air Leachate and Soilmentioning

confidence: 99%

Uncontrolled Post-Industrial Landfill—Source of Metals, Potential Toxic Compounds, Dust, and Pathogens in Environment—A Case Study

Szulc,

Okrasa,

Nowak

et al. 2024

Molecules

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The aim of this case study was the evaluation of the selected metals’ concentration, potential toxic compound identification, cytotoxicity analysis, estimation of the airborne dust concentration, biodiversity, and number of microorganisms in the environment (leachate, soil, air) of the biggest uncontrolled post-industrial landfills in Poland. Based on the results obtained, preliminary solutions for the future management of post-industrial objects that have become an uncontrolled landfill were indicated. In the air, the PM1 fraction dominated, constituting 78.1–98.2% of the particulate matter. Bacterial counts were in the ranges of 9.33 × 101–3.21 × 103 CFU m−3 (air), 1.87 × 105–2.30 × 106 CFU mL−1 (leachates), and 8.33 × 104–2.69 × 106 CFU g−1 (soil). In the air, the predominant bacteria were Cellulosimicrobium and Stenotrophomonas. The predominant fungi were Mycosphaerella, Cladosporium, and Chalastospora. The main bacteria in the leachates and soils were Acinetobacter, Mortierella, Proteiniclasticum, Caloramator, and Shewanella. The main fungi in the leachates and soils were Lindtneria. Elevated concentrations of Pb, Zn, and Hg were detected. The soil showed the most pronounced cytotoxic potential, with rates of 36.55%, 63.08%, and 100% for the A-549, Caco-2, and A-549 cell lines. Nine compounds were identified which may be responsible for this cytotoxic effect, including 2,4,8-trimethylquinoline, benzo(f)quinoline, and 1-(m-tolyl)isoquinoline. The microbiome included bacteria and fungi potentially metabolizing toxic compounds and pathogenic species.

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External sodium acetate improved Cr(VI) stabilization in a Cr-spiked soil during chemical-microbial reduction processes: Insights into Cr(VI) reduction performance, microbial community and metabolic functions

Wang

Sun

et al. 2023

Ecotoxicology and Environmental Safety

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Proteiniclasticum sediminis sp. nov., an obligate anaerobic bacterium isolated from anaerobic sludge

Cited by 6 publications

References 25 publications

Novel Bifunctional Amidase Catalyzing the Degradation of Propanil and Aryloxyphenoxypropionate Herbicides in Rhodococcus sp. C-1

Novel Bifunctional Amidase Catalyzing the Degradation of Propanil and Aryloxyphenoxypropionate Herbicides in Rhodococcus sp. C-1

Uncontrolled Post-Industrial Landfill—Source of Metals, Potential Toxic Compounds, Dust, and Pathogens in Environment—A Case Study

External sodium acetate improved Cr(VI) stabilization in a Cr-spiked soil during chemical-microbial reduction processes: Insights into Cr(VI) reduction performance, microbial community and metabolic functions

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