Proteins and cholesterol lipid vesicles are mediators of drug release from thermosensitive liposomes

Hossann, Martin; Syunyaeva, Zulfiya; Schmidt, Rebecca; Zengerle, Anja; Eibl, Hansjörg; Issels, Rolf D.; Lindner, Lars H.

doi:10.1016/j.jconrel.2012.06.032

Cited by 69 publications

(49 citation statements)

References 37 publications

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“…Lipid-grafted PEG (eg, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy(PEG)-2000, DSPE-PEG 2000 , Figure 5A) is commonly used in liposomes to create a steric barrier for inhibition of uptake by the reticuloendothelial system and increased blood circulation time, [47][48][49] but also potentially affects vesicle stability ( Figure 5B). 50 In addition to phospholipid composition, the manifestation of heat-triggered drug release depends to some degree on the drug molecule encapsulated ( Figure 4C), 28,51,52 vesicle size, 51 and the presence of serum components. 45,52 In vitro and in vivo behavior of selected formulations Until now distinct liposomal formulations have been described, which will be discussed in detail in this section (Table 1).…”

Section: Influence Of Lipid Composition On Drug Releasementioning

confidence: 99%

“…50 In addition to phospholipid composition, the manifestation of heat-triggered drug release depends to some degree on the drug molecule encapsulated ( Figure 4C), 28,51,52 vesicle size, 51 and the presence of serum components. 45,52 In vitro and in vivo behavior of selected formulations Until now distinct liposomal formulations have been described, which will be discussed in detail in this section (Table 1).…”

Section: Influence Of Lipid Composition On Drug Releasementioning

confidence: 99%

“…46 DPPG 2 -TSL showed improved in vitro stability in complete serum when compared with LTSL. 52 The presence of serum components at 37°C stabilized the formulation over time, whereas the opposite was found for LTSL. Interestingly, the lipid composition of a TSL formulation markedly influenced the effect of serum components on vesicle stability.…”

mentioning

confidence: 97%

“…Interestingly, the lipid composition of a TSL formulation markedly influenced the effect of serum components on vesicle stability. DPPC/ DSPC/DSPE-PEG 2000 80:15:5 (mol/mol) (Stealth TSL) and LTSL were more susceptible towards destabilizing effects by cholesterol-containing vesicles, 52 whereas the presence of immunoglobulin type G stronger affected the stability of DPPG 2 -TSL. 52 Moreover, the stability of DPPG 2 -TSL was less affected by size changes in the range of 100-150 nm compared to surfactant containing LTSL.…”

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confidence: 99%

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Thermosensitive liposomal drug delivery systems: state of the art review

Kneidl¹,

Peller²,

Winter³

et al. 2014

IJN

Self Cite

148

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Thermosensitive liposomes are a promising tool for external targeting of drugs to solid tumors when used in combination with local hyperthermia or high intensity focused ultrasound. In vivo results have demonstrated strong evidence that external targeting is superior over passive targeting achieved by highly stable long-circulating drug formulations like PEGylated liposomal doxorubicin. Up to March 2014, the Web of Science listed 371 original papers in this field, with 45 in 2013 alone. Several formulations have been developed since 1978, with lysolipid-containing, low temperature-sensitive liposomes currently under clinical investigation. This review summarizes the historical development and effects of particular phospholipids and surfactants on the biophysical properties and in vivo efficacy of thermosensitive liposome formulations. Further, treatment strategies for solid tumors are discussed. Here we focus on temperature-triggered intravascular and interstitial drug release. Drug delivery guided by magnetic resonance imaging further adds the possibility of performing online monitoring of a heating focus to calculate locally released drug concentrations and to externally control drug release by steering the heating volume and power. The combination of external targeting with thermosensitive liposomes and magnetic resonance-guided drug delivery will be the unique characteristic of this nanotechnology approach in medicine.

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Section: Influence Of Lipid Composition On Drug Releasementioning

confidence: 99%

Section: Influence Of Lipid Composition On Drug Releasementioning

confidence: 99%

mentioning

confidence: 97%

mentioning

confidence: 99%

See 2 more Smart Citations

Thermosensitive liposomal drug delivery systems: state of the art review

Kneidl¹,

Peller²,

Winter³

et al. 2014

IJN

Self Cite

148

View full text Add to dashboard Cite

show abstract

“…promising way to achieve a targeted delivery by some stimulus, such as temperature [12], pH [13], magnetic field [14], or mutual combination [15,16]. Among them, magnetic TDDS is used extensively to improve the targeting property by application of an external magnetic field; consequently, the therapeutic effects of drugs can be enhanced.…”

mentioning

confidence: 99%

The in vitro and in vivo anti-tumor effects of MTX-Fe3O4-PLLA-PEG-PLLA microspheres prepared by suspension-enhanced dispersion by supercritical CO2

Chen

Ting-ting

Wang

et al. 2014

Sci. China Life Sci.

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The in vitro and in vivo anti-tumor efficacy of methotrexate-loaded Fe 3 O 4 -poly-L-lactide-poly(ethylene glycol)-poly-L-lactide magnetic composite microspheres (MTX-Fe 3 O 4 -PLLA-PEG-PLLA MCMs, MMCMs), which were produced by co-precipitation (C) and microencapsulation (M) in a supercritical process, was evaluated at various levels: cellular, molecular, and integrated. The results at the cellular level indicate that MMCMs (M) show a better anti-proliferation activity than raw MTX and could induce morphological changes of cells undergoing apoptosis. At the molecular level, MMCMs (M) lead to a significantly higher relative mRNA expression of bax/bcl-2 and caspase-3 than MMCMs (C) at 10 µg mL 1 (P<0.01); and the pro-caspase-3 protein expression measured by Western blot analysis also demonstrates that MMCMs (M) can effectively activate pro-caspase-3. At the integrated level, mice bearing a sarcoma-180 tumor are used; in vivo anti-tumor activity tests reveal that MMCMs (M) with magnetic induction display a much higher tumor suppression rate and lower toxicity than raw MTX. Pharmacokinetic studies show that MMCMs (M) with magnetic induction significantly increase the accumulation of MTX in the tumor tissue compared with the other treatments. These results suggest that the MMCMs (M) prepared by the SpEDS process have great potential to play a positive role in the magnetic targeted therapy field.

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From Enzyme Encapsulation to Environmental Solutions: Photostable Laccase in Dopc Vesicles

Luna,

Reynoso,

Biasutti

et al. 2024

Eur J Org Chem

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The laccase enzyme offers significant advantages for the removal of organic contaminants; but its stability and effectiveness in bioremediation can be compromised by photo‐induced degradation processes. To address this challenge, we investigated the immobilization of laccase in unilamellar and multilamellar vesicles of dioleoyl phosphatidylcholine (DOPC) as a means to enhance its photo‐stability while preserving its catalytic activity. Enzymatic activity was evaluated spectroscopically for both free and encapsulated laccase, before and after UVB irradiation. Our results demonstrate that DOPC vesicles effectively encapsulate the laccase enzyme, maintaining its activity within the vesicles. Moreover, the substrate syringaldazine, a model compound for laccase activity studies, was able to permeate through the bilayer. Notably, encapsulated laccase exhibited significantly higher retention of enzymatic activity (REA) post‐irradiation compared to the free enzyme. In conclusion, both unilamellar and multilamellar vesicles provide substantial photoprotection for laccase against UVB light. This protective effect is attributed to the enzyme's confinement within a system that scatters high‐energy photons. Furthermore, the simplicity and ease of large‐scale production of multilamellar vesicles present an advantageous option for various applications, including bioremediation.

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Proteins and cholesterol lipid vesicles are mediators of drug release from thermosensitive liposomes

Cited by 69 publications

References 37 publications

Thermosensitive liposomal drug delivery systems: state of the art review

Thermosensitive liposomal drug delivery systems: state of the art review

The in vitro and in vivo anti-tumor effects of MTX-Fe3O4-PLLA-PEG-PLLA microspheres prepared by suspension-enhanced dispersion by supercritical CO2

From Enzyme Encapsulation to Environmental Solutions: Photostable Laccase in Dopc Vesicles

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