Proteins and RNA sequences required for the transition of the t-Utp complex into the SSU processome

Gallagher, Jennifer E. G.

doi:10.1093/femsyr/foy120

Cited by 6 publications

(5 citation statements)

References 93 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistently, Pol5 weakly copurifies with the so-called UtpA/t-UTP subcomplex (Gallagher et al 2004;Krogan et al 2004), which is believed to be the protein module that binds first to the large pre-rRNA during the formation of early 90S preribosomal particles. UtpA association to the large pre-rRNA seems to occur cotranscriptionally and this is required for both efficient pre-rRNA transcription and recruitment of other subcomplexes to these nascent particles (Gallagher et al 2004;Pérez-Fernández et al 2007Chaker-Margot et al 2015;Zhang et al 2016;Gallagher 2019). Our data, however, indicate that Pol5 is an RRB/RiBi factor required for maturation of 60S r-subunits.…”

Section: Introductionmentioning

confidence: 57%

“…Previous work has identified Pol5 as a component of the t-Utp/UtpA subcomplex of 90S preribosomal particles (Gallagher et al 2004;Krogan et al 2004). However, more recently, it has been reported that Pol5 is rather a t-UTP/UtpA accessory factor, which dissociates from the subcomplex before the incorporation of this latter into nascent 90S preribosomal particles (Gallagher 2019). The association of Pol5 with pre-60S r-particles was not previously unveiled despite the abundant information available on the composition of different affinity-purified pre-60S complexes (e.g., Fromont-Racine et al 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Delayed processing of 35S pre-rRNA, which is common for practically all loss-of-function mutations in genes involved in 60S r-subunit biogenesis including 60S r-proteins, has previously been well discussed (e.g., Axt et al 2014;Espinar-Marchena et al 2016;Talkish et al 2016) and arises as the consequence of a functional limitation of early trans-acting factors that are sequestered on aberrant pre-60S intermediates and/or the specific switch of pre-rRNA processing to the so-called A 3 pathway (Kos-Braun et al 2017). Recently, Gallagher has reported comparable results after assessing the steady-state levels of pre-rRNAs from an equivalent GAL::POL5 strain shifted to glucose medium for 24 h (Gallagher 2019). It is unlikely that Pol5 plays a direct role in 27SB pre-rRNA processing.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Pol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation in Saccharomyces cerevisiae

Ramos-Sáenz

González-Álvarez

Rodríguez-Galán

et al. 2019

RNA

View full text Add to dashboard Cite

In Saccharomyces cerevisiae, more than 250 trans-acting factors are involved in the maturation of 40S and 60S ribosomal subunits. The expression of most of these factors is transcriptionally coregulated to ensure correct ribosome production under a wide variety of environmental and intracellular conditions. Here, we identified the essential nucleolar Pol5 protein as a novel trans-acting factor required for the synthesis of 60S ribosomal subunits. Pol5 weakly and/or transiently associates with early to medium pre-60S ribosomal particles. Depletion of and temperature-sensitive mutations in Pol5 result in a deficiency of 60S ribosomal subunits and accumulation of half-mer polysomes. Both processing of 27SB pre-rRNA to mature 25S rRNA and release of pre-60S ribosomal particles from the nucle(ol)us to the cytoplasm are impaired in the Pol5depleted strain. Moreover, we identified the genes encoding ribosomal proteins uL23 and eL27A as multicopy suppressors of the slow growth of a temperature-sensitive pol5 mutant. These results suggest that Pol5 could function in ensuring the correct folding of 25S rRNA domain III; thus, favoring the correct assembly of these two ribosomal proteins at their respective binding sites into medium pre-60S ribosomal particles. Pol5 is homologous to the human tumor suppressor Myb-binding protein 1A (MYBBP1A). However, expression of MYBBP1A failed to complement the lethal phenotype of a pol5 null mutant strain though interfered with 60S ribosomal subunit biogenesis.

show abstract

Section: Introductionmentioning

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Pol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation in Saccharomyces cerevisiae

Ramos-Sáenz

González-Álvarez

Rodríguez-Galán

et al. 2019

RNA

View full text Add to dashboard Cite

show abstract

“…The downregulation of ribosome biogenesis is the first step in stress response such as starvation, heat, or chemical. To respond to stress, energy-intensive functions are down-regulated and inhibition of rRNA occurs in less than ten minutes of dextrose depletion [58].…”

Section: Discussionmentioning

confidence: 99%

Effects of MCHM on yeast metabolism

Pupo

Gallagher

2019

PLoS ONE

Self Cite

View full text Add to dashboard Cite

On January 2014 approximately 10,000 gallons of crude 4-Methylcyclohexanemethanol (MCHM) and propylene glycol phenol ether (PPH) were accidentally released into the Elk River, West Virginia, contaminating the tap water of around 300,000 residents. Crude MCHM is an industrial chemical used as flotation reagent to clean coal. At the time of the spill, MCHM's toxicological data were limited, an issue that has been addressed by different studies focused on understanding the immediate and long-term effects of MCHM on human health and the environment. Using S. cerevisiae as a model organism we study the effect of acute exposure to crude MCHM on metabolism. Yeasts were treated with MCHM 550 ppm in YPD for 30 minutes. Polar and lipid metabolites were extracted from cells by a chloroform-methanol-water mixture. The extracts were then analyzed by direct injection ESI-MS and by GC-MS. The metabolomics analysis was complemented with flux balance analysis simulations done with genome-scale metabolic network models (GSMNM) of MCHM treated vs non-treated control. We integrated the effect of MCHM on yeast gene expression from RNA-Seq data within these GSMNM. A total of 215 and 73 metabolites were identified by the ESI-MS and GC-MS procedures, respectively. From these 26 and 23 relevant metabolites were selected from ESI-MS and GC-MS respectively, for 49 unique compounds. MCHM induced amino acid accumulation, via its effects on amino acid metabolism, as well as a potential impairment of ribosome biogenesis. MCHM affects phospholipid biosynthesis, with a potential impact on the biophysical properties of yeast cellular membranes. The FBA simulations were able to reproduce the deleterious effect of MCHM on cellular growth and suggest that the effect of MCHM on ubiquinol:ferricytochrome c reductase reaction, caused by the under-expression of CYT1 gene, could be the driven force behind the observed effect on yeast metabolism and growth.

show abstract

“…The SSU processome is a dynamic intermediate with recruitment and dislodgement of dozens of assembly factors (Grandi et al ., 2002; Kornprobst et al ., 2016). A series of protein complexes (UTP‐A, UTP‐B, and UTP‐C), U3 sno‐ribonucleoprotein (U3 snoRNP), Mpp10 complex, Rcl1‐Bms1 heterodimer, and individual RBFs sequentially assemble with the transcribed 5′ETS and 18S rRNA regions, which reduces the conformational freedom of the SSU processome and facilitates the maturation of 18S rRNA (Barandun et al ., 2018; Gallagher, 2019). Among the Utp complexes, the UTP‐C complex is the least studied due to its multiple functions and heterogeneous components.…”

Section: Introductionmentioning

confidence: 99%

Defective kernel 58 encodes an Rrp15p domain‐containing protein essential to ribosome biogenesis and seed development in maize

Ma,

Liu,

Xiu

et al. 2023

New Phytologist

View full text Add to dashboard Cite

Summary Ribosome biogenesis is a highly dynamic and orchestrated process facilitated by hundreds of ribosomal biogenesis factors and small nucleolar RNAs. While many of the advances are derived from studies in yeast, ribosome biogenesis remains largely unknown in plants despite its importance to plant growth and development. Through characterizing the maize (Zea mays) defective kernel and embryo‐lethal mutant dek58, we show that DEK58 encodes an Rrp15p domain‐containing protein with 15.3% identity to yeast Rrp15. Over‐expression of DEK58 rescues the mutant phenotype. DEK58 is localized in the nucleolus. Ribosome profiling and RNA gel blot analyses show that the absence of DEK58 reduces ribosome assembly and impedes pre‐rRNA processing, accompanied by the accumulation of nearly all the pre‐rRNA processing intermediates and the production of an aberrant processing product P‐25S*. DEK58 interacts with ZmSSF1, a maize homolog of the yeast Ssf1 in the 60S processome. DEK58 and ZmSSF1 interact with ZmCK2α, a putative component of the yeast UTP‐C complex involved in the small ribosomal subunit processome. These results demonstrate that DEK58 is essential to seed development in maize. It functions in the early stage of pre‐rRNA processing in ribosome biogenesis, possibly through interacting with ZmSSF1 and ZmCK2α in maize.

show abstract

Proteins and RNA sequences required for the transition of the t-Utp complex into the SSU processome

Cited by 6 publications

References 93 publications

Pol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation in Saccharomyces cerevisiae

Pol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation in Saccharomyces cerevisiae

Effects of MCHM on yeast metabolism

Defective kernel 58 encodes an Rrp15p domain‐containing protein essential to ribosome biogenesis and seed development in maize

Contact Info

Product

Resources

About