Proteins and trypsin inhibitors in seeds of various plants

Adenanthera pavonina, a potential plant-based protein resource: Seed protein composition and immunohistochemical localization of trypsin inhibitors

“…Previous studies have shown that seeds of A. pavonina contain high levels of trypsin inhibitor activity ( Prabhu and Pattabiraman, 1980 , Sowbaghya et al, 2019 ). To partially purify and characterize the trypsin inhibitors from the seeds of A. pavonina we initially fractionated the albumin protein fraction with CaCl 2 .…”

“…Although the preliminary results are encouraging, the presence of high levels of trypsin inhibitor in A. pavonina seeds could pose a problem for its widespread use. The trypsin inhibitor activity in A. pavonina seeds is about 3–16-fold higher than that in soybean ( Sowbaghya et al, 2019 ). Therefore, it will be critical to significantly lower or inactivate the trypsin inhibitor content of A. pavonina seeds to enhance its nutritional value.…”

“…Legume seeds not only have high protein content but also accumulate significant amount of anti-nutritional factors such as trypsin inhibitors. Earlier work has shown that the seeds of A. pavonina contain very high levels of trypsin inhibitor activity ( Prabhu and Pattabiraman, 1980 , Sowbaghya et al, 2019 ). A comparison of trypsin inhibitor activity from several plant sources demonstrated that seeds of A. pavonina had the highest activity which was estimated to be about 3–16-times higher than that is present in soybean seeds ( Prabhu and Pattabiraman, 1980 , Sowbaghya et al, 2019 ).…”

“…Earlier work has shown that the seeds of A. pavonina contain very high levels of trypsin inhibitor activity ( Prabhu and Pattabiraman, 1980 , Sowbaghya et al, 2019 ). A comparison of trypsin inhibitor activity from several plant sources demonstrated that seeds of A. pavonina had the highest activity which was estimated to be about 3–16-times higher than that is present in soybean seeds ( Prabhu and Pattabiraman, 1980 , Sowbaghya et al, 2019 ). Several researchers have isolated and purified trypsin inhibitors from the seeds of A. pavonina ( Prabhu and Pattabiraman, 1980 , Richardson et al, 1986 , Souza et al, 2016 ; Chandrashekharaiah, Shashank, Bharadwaj, Raju, Ramachandra Swamy, 2017).…”

Assessment of Cytotoxic Effects of Plant Protease Inhibitors on Cancerous Cell Lines

“…Previous research has identified trypsin inhibitors in many food plants like potatoes (Solanum tuberosum), sweet corn (Zea mays), sweet potato (Ipomea batatus), spinach (Spinacia oleracea), broccoli (Brassica oleracea var.botrytis), Brussels sprouts (Brassica oleracea var. gemmifera) [25], cucumber (Cucumis sativus) [25,26], red bryony (Bryonia diotica), figleaf gourd (Cucurbita ficifolia), spaghetti squash (Cucurbita pepo), water melon (Citrullus vulgaris) [26] and bitter gourd (Momordica charantia L.) [27], green gram (Vigna radita), cashew nuts (Anacardium occidentale), jack fruit seeds (Artocarpus heterophyllus) [28] and other plants like red lucky seed (Adenanthera pavonina L.), babul (Acacia nilotica L.) [27 ] , Dalbergia latifolia (85.09±0.40%) [29], etc. Trypsin inhibitors have been also isolated from sweet corn [25] from the seeds of cucumber (Cucumis sativus), red bryony (Bryonia diotica), figleaf gourd (Cucurbita ficifolia), spaghetti squash (Cucurbita pepo) and water melon (Citrullus vulgaris) [26], Spatholobus parviflorus [30] Moringa oleifera [31,32] etc.…”