Proteins involved in electron transfer to Fe(III) and Mn(IV) oxides by Geobacter sulfurreducens and Geobacter uraniireducens

Aklujkar, Muktak; Coppi, Maddalena V.; Leang, Ching; Kim, Byoung Chan; Chavan, Milind A.; Perpetua, Lorrie A.; Giloteaux, Ludovic; Liu, A; Holmes, Dawn E.

doi:10.1099/mic.0.064089-0

Cited by 164 publications

(147 citation statements)

References 96 publications

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“…This operon consists of genes coding for a tetraheme c-type cytochrome, an iron-sulfur cluster-binding protein (Ferp_0647, Ferp_1268, and Ferp_1438), and a putative cytoplasmic membrane-associated b-type cytochrome (Ferp_0646, Ferp_1269, and Ferp_1437) with quinone-binding site motifs (45). It is remarkable that F. placidus possesses these three putative menaquinol: ferricytochrome c oxidoreductases of the type known as Cbc4 in Geobacter species (46). These Cbc4 complexes may be the primary interfaces for electrons from the oxidation of organic compounds in the cytoplasm, carried by menaquinone, to reach periplasmic c-type cytochromes in both Bacteria and Archaea en route to extracellular electron acceptors such as Fe(III).…”

Section: Resultsmentioning

confidence: 99%

“…Differential expression of c-type cytochromes during growth with soluble or insoluble Fe(III) as electron acceptor. Previous studies with Fe(III)-reducing bacteria, including Geobacter and Shewanella species, have shown that these organisms express different c-type cytochrome and electron transport proteins when grown on soluble and insoluble electron acceptors (9,(46)(47)(48). Proteomic and transcriptomic studies were done to see if F. placidus also differentially expresses c-type cytochromes and other electron transport proteins when electron acceptors are varied.…”

Section: Resultsmentioning

confidence: 99%

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Mechanisms Involved in Fe(III) Respiration by the Hyperthermophilic Archaeon Ferroglobus placidus

Smith

Aklujkar

Risso

et al. 2015

Appl Environ Microbiol

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The hyperthermophilic archaeon Ferroglobus placidus can utilize a wide variety of electron donors, including hydrocarbons and aromatic compounds, with Fe(III) serving as an electron acceptor. In Fe(III)-reducing bacteria that have been studied to date, this process is mediated by c-type cytochromes and type IV pili. However, there currently is little information available about how this process is accomplished in archaea. In silico analysis of the F. placidus genome revealed the presence of 30 genes coding for putative c-type cytochrome proteins (more than any other archaeon that has been sequenced to date), five of which contained 10 or more heme-binding motifs. When cell extracts were analyzed by SDS-PAGE followed by heme staining, multiple bands corresponding to c-type cytochromes were detected. Different protein expression patterns were observed in F. placidus cells grown on soluble and insoluble iron forms. In order to explore this result further, transcriptomic studies were performed. Eight genes corresponding to multiheme c-type cytochromes were upregulated when F. placidus was grown with insoluble Fe(III) oxide compared to soluble Fe(III) citrate as an electron acceptor. Numerous archaella (archaeal flagella) also were observed on Fe(III)-grown cells, and genes coding for two type IV pilin-like domain proteins were differentially expressed in Fe(III) oxidegrown cells. This study provides insight into the mechanisms for dissimilatory Fe(III) respiration by hyperthermophilic archaea.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Mechanisms Involved in Fe(III) Respiration by the Hyperthermophilic Archaeon Ferroglobus placidus

Smith

Aklujkar

Risso

et al. 2015

Appl Environ Microbiol

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“…Several other cytochrome c-containing and biogenesis proteins appear to be regulated by cAG, including OmcS, which has been shown to be essential for metal oxide reduction and more highly expressed upon growth on insoluble Fe(III) or Mn(IV) oxides (28,29). Although cytochrome genes are remarkably abundant in Geobacter species, they are not well conserved, making it difficult to base functional assignments of electron conduits on phylogenetic arguments (30).…”

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confidence: 99%

GEMM-I riboswitches from Geobacter sense the bacterial second messenger cyclic AMP-GMP

Kellenberger¹,

Wilson²,

Hickey³

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

121

146

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Cyclic dinucleotides are an expanding class of signaling molecules that control many aspects of bacterial physiology. A synthase for cyclic AMP-GMP (cAG, also referenced as 3′-5′, 3′-5′ cGAMP) called DncV is associated with hyperinfectivity of Vibrio cholerae but has not been found in many bacteria, raising questions about the prevalence and function of cAG signaling. We have discovered that the environmental bacterium Geobacter sulfurreducens produces cAG and uses a subset of GEMM-I class riboswitches (GEMM-Ib, Genes for the Environment, Membranes, and Motility) as specific receptors for cAG. GEMM-Ib riboswitches regulate genes associated with extracellular electron transfer; thus cAG signaling may control aspects of bacterial electrophysiology. These findings expand the role of cAG beyond organisms that harbor DncV and beyond pathogenesis to microbial geochemistry, which is important to environmental remediation and microbial fuel cell development. Finally, we have developed an RNA-based fluorescent biosensor for live-cell imaging of cAG. This selective, genetically encodable biosensor will be useful to probe the biochemistry and cell biology of cAG signaling in diverse bacteria.

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“…The cbcY gene that encodes for the Cbc1 enzyme, a enaquinol; ferricytochrome c oxioreductase was found to be downregulated on growth with both Fe(III) and Mn(IV) oxides and the gene that encodes for the periplasmic dihaem cytochrome c peroxidase, ccpA was found to be upregulated on growth with both Fe(III) and Mn(IV) oxides [185]. In order to explain these findings, the following paragraphs will describe what is presented in the current literature.…”

Section: Abundance Of Eet Related Proteinsmentioning

confidence: 99%

“…In a study comparing the metabolic status of cells growing close to an anode electrode versus cells in the outer portion of the anode biofilm, the gene GSU2743 a homologue of KN400_2682 was found to be decreased in the outer biofilm and was said to not play a direct role in EET [184]. A study observing the proteins involved in electron transfer between Fe(III) and Mn(IV) oxides as well as a soluble electron accepter Fe(III) citrate found that the genes GSU2732 (KN400_2674) and PgcA had higher transcript levels higher on growth on Mn(IV) oxide vs soluble Fe(III) citrate however this was not the case with Fe(III) oxide [185].…”

Section: Abundance Of Eet Related Proteinsmentioning

confidence: 99%

The mechanisms of extracellular electron transfer

Grobbler¹

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In bioelectrochemical systems (BESs) microbial activity facilitates electricity generation and product synthesis. Using the microbial process of extracellular electron transfer (EET) Shewanella and Geobacter species can respire using a solid terminal electron acceptor, such as an anode in BES. Study of these microorganisms and how they behave at the molecular level is important for shining light on geomicrobial processes and development of BES. Through the use of molecular and electrochemical techniques, this PhD thesis will focus on the molecular mechanisms employed by bacterial biofilms on the anode of a BES, specifically Shewanella oneidensis MR-1 and Geobacter sulfurreducens DL-1. The physiology of these microorganisms appears to be directly associated with the operational conditions of the BES. The application of electrochemical and molecular studies enables the comparison and understanding of the cellular response to the BES operation, in particular on how they respond to changes in the anode potential. Quantitative proteomics from low biomass, biofilm samples is not well documented.

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Proteins involved in electron transfer to Fe(III) and Mn(IV) oxides by Geobacter sulfurreducens and Geobacter uraniireducens

Cited by 164 publications

References 96 publications

Mechanisms Involved in Fe(III) Respiration by the Hyperthermophilic Archaeon Ferroglobus placidus

Mechanisms Involved in Fe(III) Respiration by the Hyperthermophilic Archaeon Ferroglobus placidus

GEMM-I riboswitches from Geobacter sense the bacterial second messenger cyclic AMP-GMP

The mechanisms of extracellular electron transfer

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