Proteins involved in flor yeast carbon metabolism under biofilm formation conditions

Proteins involved in wine aroma compounds metabolism by a Saccharomyces cerevisiae flor-velum yeast strain grown in two conditions

“…Yeast cells from both media were centrifuged in a Rotina-38 centrifuge at 4500 g for 10 min and rinsed with sterile distilled water at 4 C. The cell pellets thus obtained were subjected to protein extraction, followed by OFFGEL protein fractionation, LTQ Orbitrap MS analysis and quantitation in terms of the exponentially modified protein abundance index (emPAI; Ishihama et al, 2005). These procedures and methods are described in detail elsewhere (Moreno-García et al, 2015).…”

Differential Proteome Analysis of a Flor Yeast Strain under Biofilm Formation

“…Ratios above two were also reported for glycolysis and glucose fermentation pathways (Table 3). Ten of the fifteen glycolytic proteins detected under BFC were also involved in the gluconeogenetic process required for yeast growth on ethanol or glycerol as the carbon source [10]. This may account for the large number of proteins found under BFC.…”

“…Data treated in the present manuscript were extracted from Moreno-García et al [10], where the Saccharomyces cerevisiae flor yeast strain, inoculum and cultivation conditions are described. In order to follow dissolved oxygen content in the medium, the Oxymeter device CRIMSON OXI 92 was used.…”

“…Methods for the collection of the cells and protein extraction, fractionation, identification and quantification are indicated in Moreno-García et al [4,10]. …”

“…This study is part of a sequence of proteomic research that was focused on specific flor yeast organelles/processes [4,10,11,12]. Distinctively, a broad vision of the flor yeast proteome is provided, and those organelles, biological processes and pathways with more proteins and in higher contents are highlighted in this work.…”