2021
DOI: 10.1016/j.jprot.2021.104335
|View full text |Cite
|
Sign up to set email alerts
|

Proteins involved in mitochondrial metabolic functions and fertilization predominate in stallions with better motility

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
6
0

Year Published

2022
2022
2024
2024

Publication Types

Select...
6

Relationship

3
3

Authors

Journals

citations
Cited by 10 publications
(6 citation statements)
references
References 62 publications
0
6
0
Order By: Relevance
“…Recent proteomics studies link higher amounts of the enzyme fructose bisphosphate aldolase and poor motility and velocities; this is the enzyme involved in splits of 1, 6 fructose bisphosphate in dihydroxyacetone phosphate (DHAP) and glyceraldehyde 3-phosphate (G3P), are precursors of G and MG (49). Production of G, and especially MG was concomitant with reduced motility and sperm velocities, a drop in mitochondrial membrane potential, and an increment of the production of ROS (49,125).…”
Section: Metabolic Disfunction Production Of Ros In the Spermatozoamentioning
confidence: 99%
“…Recent proteomics studies link higher amounts of the enzyme fructose bisphosphate aldolase and poor motility and velocities; this is the enzyme involved in splits of 1, 6 fructose bisphosphate in dihydroxyacetone phosphate (DHAP) and glyceraldehyde 3-phosphate (G3P), are precursors of G and MG (49). Production of G, and especially MG was concomitant with reduced motility and sperm velocities, a drop in mitochondrial membrane potential, and an increment of the production of ROS (49,125).…”
Section: Metabolic Disfunction Production Of Ros In the Spermatozoamentioning
confidence: 99%
“…The separation and analysis were conducted in an Agilent 1290 Infinity II Series UHPLC (Agilent Technologies, Santa Clara, CA, USA) equipped with an automated multisampler module and a High Speed Binary Pump, coupled to a Mass Spectrometer (Agilent 6550 Q-TOF, Agilent Technologies, Santa Clara, CA, USA) using an Agilent Jet Stream Dual electrospray (AJS-Dual ESI) interface, controlled by the MassHunter Workstation Data Acquisition software (Agilent Technologies, Rev. B.06.01), following previously published protocols [ 2 , 4 , 5 , 9 ]. The digested proteins were injected onto an Agilent Advance Bio Peptide Mapping HPLC column (2.7 μm, 150 × 2.1 mm, Agilent technologies), at 55 °C, at a flow rate of 0.4 ml/min.…”
Section: Experimental Design Materials and Methodsmentioning
confidence: 99%
“…This information, after re-analysis, may be used to improve our understanding of the regulation of stallion sperm motility, and to develop on farm tests to check sperm quality. The data set has been deposited to the ProteomeXchange Consortium [2] via the PRIDE partner repository with the dataset identifier PRIDE PXD025807 , https://www.ebi.ac.uk/pride/archive/projects/PXD025807…”
Section: Data Descriptionmentioning
confidence: 99%
“…A noteworthy finding of recent proteomic studies is a molecular explanation for the link between sperm motility and velocities, metabolism, and ability to fertilize [5]. In stallions showing better percentages of motility, circular and average velocity, mitochondrial proteins with roles in the citric acid cycle, pyruvate metabolism, and oxidative phosphorylation predominate.…”
Section: Proteomics In the Improvement Of Stallion Sperm Biotechnologiesmentioning
confidence: 99%
“…As far as the authors know, the first comprehensive study of the proteome of the stallion spermatozoa was conducted by Australian researchers at the University of Newcastle [2]. Since this first study, proteomic and phospho-proteomic approaches have been introduced to the study of stallion sperm biology [3][4][5][6][7][8][9][10][11]. This review aims to provide an updated overview of the major findings and advances in the comprehension of the stallion spermatozoa obtained thanks to the introduction of proteomics to the study of stallion sperm biology, and the impact of these findings on sperm biotechnology in equines.…”
mentioning
confidence: 99%