Proteins of rat serum V: Adjuvant arthritis and its modulation by nonsteroidal anti-inflammatory drugs

Abstract

The effect of adjuvant arthritis (AA) on the pattern of rat serum proteins includes the upregulation of haptoglobin, orosomucoid, alpha2-macroglobulin, serine protease inhibitor-3, thiostatin, alpha1-antitrypsin, C-reactive protein, and the downregulation of kallikrein-binding protein, alpha1-inhibitor III, apolipoprotein A-I, alpha2-HS-glycoprotein, albumin, apolipoprotein A-IV, transthyretin and transferrin. Minor changes (+/- 20%) are observed for Gc-globulin, ceruloplasmin, and alpha1-macroglobulin. AA thus grossly resembles the acute inflammatory response elicited by the injection of turpentine, although the changes in the levels of negative acute-phase proteins (APP) are smaller in acute inflammation. Indomethacine and ibuprofen inhibit the effects of arthritis on the synthesis of rat serum proteins in different ways: The former is, on average, three times as effective as the latter. Each drug interferes differently with different proteins. In animals without AA, both nonsteroidal anti-inflammatory drugs (NSAID) mimic the inflammatory pattern to a certain extent, with more effect on the negative than on the positive APPs. Overall, the shifts in serum protein levels parallel changes in inflammatory parameters such as joint swelling and serum interleukin-6 (IL-6) activity. Protein quantitation after two-dimensional electrophoresis (2-DE) reveals some effects of the drugs per se which escape detection by other routine tests.

Proteomic analysis of differentially expressed serum proteins in lung cancer

“…Studies by Gianazza et al on rat serum identified 34 proteins with human homologues and concluded that even abundant proteins could be markers for disease states [30][31][32]. Traditionally, it was believed that the only source of Immunoglobulins (IgGs) was mature B lymphocytes, but recently, some researchers have reported that IgG could also be detected in carcinoma cells derived from epithelium [33].…”

Proteins of rat serum, urine, and cerebrospinal fluid: VI. Further protein identifications and interstrain comparison

“…Most analytical procedures were as previously described [1,2,4,5,8]. 2-DE maps were obtained by IPG-DALT [9].…”

“…[26], provides no information of the possible influence of ethnic origin, although data are subdivided by gender and age. An extensive survey on minor protein components in human serum has been undertaken by Leigh Anderson et al (oral communication at 4th Siena Meeting "From Genome to Proteome: Knowledge Acquisition and Representation", Sept. [4][5][6][7]2000). When testing over a larger genetic distance (Mus musculus and Mus spretus vs. two strains), Klose and co-workers detected more than 1000 variant protein spots (in brain, heart, and skeletal muscle) between the two subspecies [27,28].…”

“…Subsequently applying our results to pharmacological testing, we were able to define the presence, intensity and detailed pattern of an inflammatory condition in several animal models [4][5][6]. Some of the latter investigations require analysis of samples from rat strains other than Sprague-Dawley, and from additional biological fluids.…”

Proteomics of rat biological fluids — The tenth anniversary update

“…Analysis of rat serum by 2-DE thus provides a very sensitive way to monitor onset and progression of any pathological state involving an inflammatory component as well as to assess the outcome of any therapeutic intervention. We could validate such principles in a few model systems (various types of experimentallyinduced inflammation [5][6][7], a genetically-determined susceptibility to cerebro-vascular disease [8][9][10][11]). We could then apply similar concepts to the analysis of cerebrospinal fluid (CSF) proteome [12].…”