Proteins of seminal fluid and spermatozoa in the trout (Oncorhynchus mykiss): Partial characterization and variations

Loir, Maurice; Labbé, Catherine; Maisse, Gérard; Pinson, A.; Boulard, Geneviève; Mourot, Brigitte; Chambeyron, F.

doi:10.1007/bf00003405

Cited by 67 publications

(64 citation statements)

References 23 publications

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“…Generally, interactions of ions present in the seminal plasma with the sperm membrane influence the membrane potential and represent a mechanism of inhibition of spermatozoa in the seminal plasma or sperm duct (Boitano and Omoto, 1991), allowing to control the initiation of sperm motility after release to the surrounding medium (Krasznai et al, 2000). The total protein concentration in the present study (0.1-0.9, 0.4 ± 0.2 mg/ml) was lower than that reported for salmonids such as rainbow trout: 1.74 ± 0.79 mg/ml (Loir et al, 1990), 1.34 ± 0.67 mg per ml (Ciereszko and Dabrowski, 1993) and 1.47 ± 0.84 mg/ml (Lahnsteiner et al, 1998). In addition, the protein content is significantly different from that of non-salmonid fish (Piironen and Hyvarinen, 1983;Lahnsteiner et al, 1995;Lahnsteiner et al, 1996).…”

Section: Organic and Inorganic Components Of Seminal Plasmacontrasting

confidence: 70%

Evaluation of semen quality of endangered Caspian brown trout (Salmo trutta caspius) in different times of spermiation during the spawning season

Hajirezaee¹,

Amiri²,

Mirvaghefi³

et al. 2010

CZECH J ANIM SCI

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Sperm motility and sperm production as well as organic and inorganic components present in the seminal plasma were measured at different time periods of spermiation during the spawning season in order to evaluate the semen quality in Caspian brown trout, Salmo trutta caspius. For such evaluation, males were divided into four groups based on the date of spermiation: group A (pre-mature broodstocks), group B and C (mid-mature broodstocks), group D (late-mature broodstocks). Our results showed that the Caspian brown trout produces semen of variable quality depending on the date in the period of spermiation. In this regard, the percentage of motile spermatozoa, duration of motility, sperm density, osmolality and also the concentrations of Na + , Cl -, K + , Ca 2+ , Mg 2+ and total protein content were significantly higher in mid-mature broodstocks (which matured in the middle of spawning season, i.e. groups B and C) compared to pre-mature (group A) and late-mature (group D) samples. Statistically significant positive correlations were also found between these semen parameters: sperm production vs Na

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Section: Organic and Inorganic Components Of Seminal Plasmacontrasting

confidence: 70%

Evaluation of semen quality of endangered Caspian brown trout (Salmo trutta caspius) in different times of spermiation during the spawning season

Hajirezaee¹,

Amiri²,

Mirvaghefi³

et al. 2010

CZECH J ANIM SCI

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“…Generally in fish, the protein concentration in seminal plasma does not exceed 2 g/l. Currently, only two protein groups were identified in fish seminal plasma: lipoproteins (Loir et al, 1990) and anti-proteinases (Ciereszko et al, 2000). Besides that, some proteolytic activity was found in fish seminal plasma (Lahnsteiner et al, Supported by UWM Olsztyn,Poland (Project No.…”

mentioning

confidence: 99%

Quality of semen and histological analysis of testes in Eurasian perch Perca fluviatilis L. during a spawning period

Król¹,

Demska‐Zakęś²,

Hliwa³

2006

CZECH J ANIM SCI

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ABSTRACT:A qualitative assessment of the Eurasian perch Perca fluviatilis semen describing the basic parameters of seminal plasma was completed. The histological methods used in this study showed changes in perch gonads during a spawning and post-spawning period. At the late period of the spawning season, the structure of testes was clearly loosened and spermatozoa did not fill uniformly all the ampullae of the testes, leaving free spaces at their banks and no spermatids were observed. The results confirmed that there was an additional period after spawning in the annual reproductive cycle of the male Eurasian perch. In both years of investigations (2000)(2001), an essential decline in sperm motility at the late period of the spawning season was observed, from more than 85% to 56% in 2000. The sperm motility was not influenced by sperm concentrations because throughout the spawning time no changes in the sperm concentration were observed, 32.4 and 32.6 mld/ml at beginning and at the late period of spawning period, respectively. In contrast to sperm concentrations, protein concentrations in seminal plasma increased in the late period of spawning season, from 3.95 to 5.16 g/l in 2001. This study confirmed that protein concentrations in the seminal plasma of most fish are much lower than in the other vertebrates. Among the fish examined, perch is characterized by one of the highest values of protein concentrations in plasma. Our observations confirmed that a high water temperature influenced anatomical and functional parameters of the reproductive system in male perch.

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“…After SDS-PAGE analysis, there is slight changes in the protein composition of membranes in the three bands. Among the proteins, the relative amount of which increases from B1 to B3, as compared to protein 42 KD, only 2 (14.5 and 29 KD) are seminal proteins adsorbed onto the gamete surface (Loir et al 1990b). The quantitative changes of these 2 proteins in the 3 bands could be due to partial removal during membrane preparation.…”

Section: Discussionmentioning

confidence: 99%

“…Membrane proteins were also present in B4, but they were mixed with proteins characteristic of flagellae, especially the main flagellar protein of 49 KD (Loir et al 1990b), which predominated in this fraction (Fig. 4E).…”

Section: Protein Analysismentioning

confidence: 98%

Plasma membrane of trout spermatozoa: I. Isolation and partial characterization

Labbé

Loir

1991

Fish Physiol Biochem

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AbslractThe plasma membrane from spermatozoa of rainbow trout was isolated by four techniques: sonication, hypotonic shock, mechanical homogenization after freeze-thawing, and nitrogen cavitation, in combination with continuous sucrose gradient centrifugation. Nitrogen cavitation (900 PSI, 20 min equilibration at 4°C) was the most effective technique.Following nitrogen cavitation, four bands were recovered in the sucrose gradient at densities = 1.03, 1.05, 1.09 and 1.15 g/ml. Elect ron microscopy revealed membrane vesicles of various sizes in bands 1 to 3, while enzyme analysis revealed a 3.9 to 5.5-fold enrichment in 5'-nucleotidase and little contamination by lactate dehydrogenase (cytosol) and succinic dehydrogenase (mitochondria). Lipid analysis of bands 1 and 2 indicated a 6 to 7-fold enrichment in cholesterol and a cholesterol: phospholipid ratio of 0.59-0.70. Seven classes of phospholipids were present in bands 1-3 with no significant differences observed among bands. These data indicate that the vesicles (in bands 1 and 2) obtained after nitrogen cavitation are primarily plasma membranes. Membranes in band 3 appear to be slightly contaminated with nuclear membranes.Most of the plasma membrane proteins were acidic to neutral. The 2 main membrane proteins were 42 and 30 Kilodaltons.

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Proteins of seminal fluid and spermatozoa in the trout (Oncorhynchus mykiss): Partial characterization and variations

Cited by 67 publications

References 23 publications

Evaluation of semen quality of endangered Caspian brown trout (Salmo trutta caspius) in different times of spermiation during the spawning season

Evaluation of semen quality of endangered Caspian brown trout (Salmo trutta caspius) in different times of spermiation during the spawning season

Quality of semen and histological analysis of testes in Eurasian perch Perca fluviatilis L. during a spawning period

Plasma membrane of trout spermatozoa: I. Isolation and partial characterization

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