Proteins of the mineral compartment of bovine fetal enamel share common antigenic determinants with serum proteins

The reason for the presence of albumin and other serum, cytoskeletal, cytosolic, and extracellular matrix proteins in enamel fractions was investigated by immunoblotting using homogenates prepared from freeze-dried and freshly dissected rat incisors, and antibodies capable of resolving at least 1 ng of the primary antigen. The data indicated that most of the 16 antibodies examined in this study reacted with antigens present only within "cell" homogenates (enamel organ cells + adhering labial connective tissue and blood vessels). One exception was rat serum albumin which was detected routinely in enamel homogenates prepared from freshly dissected, wiped incisors but rarely within enamel homogenates prepared from freeze-dried incisors. Another exception was calbindin-D 28 kDa which was consistently found within secretory stage enamel homogenates irrespective of preparative technique. A third exception was enamel proteins (amelogenins) which were enriched in secretory and early maturation stage enamel homogenates compared with cell homogenates and distributed as multiple molecular weight, antigenic bands in enamel homogenates (14-30 kDa), but mostly as a single antigenic band in cell homogenates (near 27 kDa). Overall, the results of this study suggest that developing rat incisor enamel naturally contains few exogenous proteins such as albumin. High concentrations of albumin (or other serum proteins) in crude homogenates, or purified fractions, derive mostly from blood and/or tissue fluids soaking into the enamel during sample preparation. This type of artifact can be avoided by using freeze-dried teeth for biochemical analyses.

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Immunoblotting studies on artifactual contamination of enamel homogenates by albumin and other proteins

Chen

Nanci

Smith

1995

Calcif Tissue Int

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Albumin Gene Expression During Mouse Odontogenesis

et al. 1996

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Albumin protein is present in developing teeth of several species. Oligomer primers and cRNA probes specific for albumin were designed to perform RT-PCR, and for in situ hybridization, respectively. In situ hybridization failed to reveal albumin expression in any tooth cells, however, albumin PCR products were amplified from tissues adhering to the roots of developing teeth from four-week-old mice. It is concluded that this source is not the primary source of albumin protein found in developing enamel, because of the location and level of expression of albumin mRNA in periodontal tissue.

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Ovalbumin is a Component of the Chicken Eggshell Matrix

Hincke¹

1995

Connective Tissue Research

114

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The protein components of biomineralized structures (matrix proteins) are believed to modulate crystal nucleation and growth, and thereby influence the shape and strength of the final structure. The chicken eggshell contains a complex array of distinct matrix proteins. One of these was found to have similar molecular weight and chromatographic properties as purified egg ovalbumin. A commercially available antibody to ovalbumin was utilized for western blotting to demonstrate that ovalbumin is one of the matrix proteins this is extracted from decalcified eggshell. Immunohistochemistry revealed that ovalbumin is found only in the mammillary bodies of decalcified shell, and is not distributed throughout the shell matrix. These results indicate that ovalbumin is present during the initial phase of shell formation and becomes incorporated into the protein matrix of the mammillary bodies. However, it is not yet clear whether ovalbumin at this site plays a specific role in shell mineralization.

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Proteins of the mineral compartment of bovine fetal enamel share common antigenic determinants with serum proteins

Cited by 3 publications

References 37 publications

Immunoblotting studies on artifactual contamination of enamel homogenates by albumin and other proteins

Immunoblotting studies on artifactual contamination of enamel homogenates by albumin and other proteins

Albumin Gene Expression During Mouse Odontogenesis

Ovalbumin is a Component of the Chicken Eggshell Matrix

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