Proteins with abortifacient, ribosome inactivating, immunomodulatory, antitumor and anti-AIDS activities from Cucurbitaceae plants

Ng, Tzi Bun; Chan, Wood Yee; Yeung, H.W.

doi:10.1016/0306-3623(92)90131-3

Cited by 151 publications

(61 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is well established that hydrophobic residues stabilize the interaction between nucleic acids and proteins by intercalating between bases of DNA or RNA (Frankel et al, 1989). The molecular-weight and N-terminal sequence similarities between ME2 and MAP suggest the presence of RIPs among the genus Mirabilis, similar to the widespread content of RIPs in the genus Trichosanthes (Ng et al, 1992(Ng et al, , 1993.…”

Section: Discussionmentioning

confidence: 81%

Characterization of Two Novel Type I Ribosome-Inactivating Proteins from the Storage Roots of the Andean CropMirabilis expansa1

1999

View full text Add to dashboard Cite

Two novel type I ribosome-inactivating proteins (RIPs) were found in the storage roots of Mirabilis expansa, an underutilized Andean root crop. The two RIPs, named ME1 and ME2, were purified to homogeneity by ammonium sulfate precipitation, cationexchange perfusion chromatography, and C 4 reverse-phase chromatography. The two proteins were found to be similar in size (27 and 27.5 kD) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and their isoelectric points were determined to be greater than pH 10.0. Amino acid N-terminal sequencing revealed that both ME1 and ME2 had conserved residues characteristic of RIPs. Amino acid composition and western-blot analysis further suggested a structural similarity between ME1 and ME2. ME2 showed high similarity to the Mirabilis jalapa antiviral protein, a type I RIP. Depurination of yeast 26S rRNA by ME1 and ME2 demonstrated their ribosome-inactivating activity. Because these two proteins were isolated from roots, their antimicrobial activity was tested against root-rot microorganisms, among others. ME1 and ME2 were active against several fungi, including Pythium irregulare, Fusarium oxysporum solani, Alternaria solani, Trichoderma reesei, and Trichoderma harzianum, and an additive antifungal effect of ME1 and ME2 was observed. Antibacterial activity of both ME1 and ME2 was observed against Pseudomonas syringae, Agrobacterium tumefaciens, Agrobacterium radiobacter, and others.

show abstract

Section: Discussionmentioning

confidence: 81%

Characterization of Two Novel Type I Ribosome-Inactivating Proteins from the Storage Roots of the Andean CropMirabilis expansa1

1999

View full text Add to dashboard Cite

show abstract

“…A glycoprotein was isolated from seeds of L. acutangula, which was found to be immunologically distinct from abortifacient proteins isolated from other members of the Cucurbitaceae family (Yeung et al, 1991). The luffaculin, a protein isolated from its seed, exhibited abortifacient, antitumor, ribosome inactivating and immunomodulatory activities (Ng et al, 1992). Another ribosome inactivating peptide isolated from seeds of ridge gourd designated luffangulin inhibited cell-free translation (Wang and Ng, 2002).…”

Section: Introductionmentioning

confidence: 99%

Variability, character association and diversity analysis of ridge gourd (Luffa acutangula roxb.) genotypes of Bangladesh

2014

View full text Add to dashboard Cite

Sixty genotypes of ridge gourd (Luffa acutangula Roxb.) were collected from different locations of Bangladesh and were used to study their variability, character association and genetic diversity. Seventeen traits studied, of these, eight showed high heritability (over 80%). Correlation coefficient study indicated that yield per plant had highly significant positive relationship with the number of fruits per plant (r = 0.874**), average fruit weight (r = 0.707**), vine length at harvest (r = 0.359**) and length of fruit (r = 0.334**). On the other hand, the sex ratio and circumference of fruit exhibited negative association with yield. Path co-efficient analysis showed that number of fruits per plant exhibited the highest positive direct effect (0.7192) on yield followed by average fruit weight (0.4656). Based on Mahalanobis' D 2 statistics, the sixty genotypes were grouped into four different clusters. Clustering pattern revealed that genotypes collected from the same location were grouped into different clusters. The average intercluster distances were always higher than the average intracluster distances suggesting wider genetic diversity among the genotypes within the cluster. Custer-III had the highest intracluster distance (97.9).

show abstract

“…The potential of some of the RIPs to depurinate the rRNA at multiple sites 7 along with several other polynucleotide substrates 8 has been addressed. Broad and diverse pharmacological attributes have been associated with RIPs, as protein synthesis-inhibitory, immunosuppressive, anti-tumor, antiviral, anti-HIV and abortificient traits to mention a few 9 . The fascinating multimodal activities of RIPs have since long time generated interest towards developing antitumor drugs that selectively target tumor cells 10 .…”

mentioning

confidence: 99%

Cytological and Subcellular Response of Cells Exposed to the Type-1 RIP Curcin and its Hemocompatibility Analysis

Mohamed

Veeranarayanan

Minegishi

et al. 2014

Sci Rep

View full text Add to dashboard Cite

Curcin, a type 1 ribosome inactivating protein (RIP) is investigated here for its cellular competence on six mammalian cell lines. Cells exposed to curcin (100 mg/ml) for 72 h exhibited significant cellular metabolic arrest, with the cancer cell lines being more sensitive. The viability assessment of the cancer cells in a 3D cell culture based assay revealed highly restricted sprouting and proliferation with near to complete dead cell population. Prominent mitochondrial dysfunction, elevated reactive oxygen species levels, nuclear degeneration, structural/mechanical destabilization and suppression of defense mechanisms were imminent with the RIP treated cells. Expression levels of nuclear factor kB (NF-kB), cytoskeletal focal adhesion kinases (FAK) and vinculin were significantly diminished. Vital cellular organelles as nucleus, mitochondria and actin were severely incapacitated on RIP exposure resulting in multimodal apoptosis and necrosis. The ability of curcin to impart comprehensive shutdown of the cells, especially cancer cells, complemented with its hemocompatibility, opens up possibilities of utilizing this ribotoxin as a prospective therapeutic candidate against cancers of diverse origins.

show abstract

Proteins with abortifacient, ribosome inactivating, immunomodulatory, antitumor and anti-AIDS activities from Cucurbitaceae plants

Cited by 151 publications

References 41 publications

Characterization of Two Novel Type I Ribosome-Inactivating Proteins from the Storage Roots of the Andean CropMirabilis expansa1

Characterization of Two Novel Type I Ribosome-Inactivating Proteins from the Storage Roots of the Andean CropMirabilis expansa1

Variability, character association and diversity analysis of ridge gourd (Luffa acutangula roxb.) genotypes of Bangladesh

Cytological and Subcellular Response of Cells Exposed to the Type-1 RIP Curcin and its Hemocompatibility Analysis

Contact Info

Product

Resources

About