Proteinuria in rats in relation to age-dependent renal changes

Alt, J.; Hackbarth, Hansjoachim; Deerberg, F; Stolte, Hilmar

doi:10.1258/002367780780942809

Cited by 62 publications

(20 citation statements)

References 19 publications

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“…Albumin and IgL were the only serum proteins recognized in the pattern of WKY and SHR at all time points, [21][22][23] whereas in SHRSP the proteins of high M r lost in urine during treatment were resolved by 2-dimensional electrophoretic analysis as transferrin, albumin, thiostatin, kallikrein-binding protein, ␣ 2 -HS-glycoprotein, serine protease inhibitor 3, ␣ 1 -antitrypsin, Gc-globulin, apolipoprotein A-IV, transthyretin, and the albumin fragments migrating as slanted rows with M r 50 and 30 kDa. Hemopexin is not easily recognized in Coomassie-stained patterns, but its presence was confirmed by immunoblotting (not shown).…”

Section: Proteomics Datamentioning

confidence: 85%

Acute-Phase Proteins Before Cerebral Ischemia in Stroke-Prone Rats

Sironi

Tremoli

Miller

et al. 2001

Stroke

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Background and Purpose-A high degree of proteinuria has been reported in stroke-prone spontaneously hypertensive rats (SHRSP). We studied the effect of salt loading on the detailed protein pattern of serum and urine in 3 rat strains: Wistar-Kyoto, spontaneously hypertensive rats, and SHRSP, an inbred animal model for a complex form of cerebrovascular disorder resembling the human disease. Methods-Rats were given a permissive diet and received 1% NaCl in drinking water. The protein pattern in body fluids was assessed over time by 2-dimensional electrophoretic analysis. Brain alterations were monitored by MRI and histology. Results-Several proteins were excreted in urine after weeks of treatment and in advance of stroke: transferrin, hemopexin, albumin, ␣ 2 -HS-glycoprotein, kallikrein-binding protein, ␣ 1 -antitrypsin, Gc-globulin, and transthyretin. Markers of an inflammatory response, including very high levels of thiostatin, were detected in the serum of SHRSP at least 4 weeks before a stroke occurred. Conclusions-In

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Section: Proteomics Datamentioning

confidence: 85%

Acute-Phase Proteins Before Cerebral Ischemia in Stroke-Prone Rats

Sironi

Tremoli

Miller

et al. 2001

Stroke

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“…The gels were stained with amido black and scanned with a Joyce-Loebl doublebeam microdensitometer. The concentration of LMW proteins, albumin and HMW proteins was esti mated by planimetry' of the protein peaks and in rela tion to an albumin standard [1], Since no specific standards for LMW and HMW proteins are available, the values for these proteins given in this paper are only estimates. This could explain the discrepancy between the sum of LMW.…”

Section: Methodsmentioning

confidence: 99%

Species and Strain Differences in Urinary Protein Excretion

Alt¹,

Maeß²,

Hackbarth

1985

Kidney Blood Press Res

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The physiological excretion of urinary protein is subject to great variation influenced not only by environmental and hormonal factors but also by genetics. The present study demonstrates that there is not only variation in respect to the excretion of plasma proteins but also of other types of proteins which are specific urinary proteins. There is a close relation between body weight and total protein excretion. However, male rats and mice do not fit the allometric line calculated for the other species studied. This is of special importance since these two species are often used in kidney research. The reason for this divergence in total protein is the excretion of sex-dependent low molecular weight proteins. The excretion of albumin, which is a marker of glomerular permeability and tubular reabsorption as well, shows a marked genetic variation between different rat strains. The data presented in this study demonstrate the general admissibility of transferring data from one species to the other but also the limitations in respect especially to the sex-dependent proteins which are excreted by some species.

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“…130 Strain characteristics MWF rats develop mild SS spontaneous hypertension, spontaneous albuminuria of early onset, structural renal abnormalities such as an inherited nephron reduction of 30-50%, glomerulosclerosis, reduced podocyte number, renal interstitial fibrosis and endothelial dysfunction. [130][131][132][133][134][135][136][137][138][139] Moreover, a sexual dimorphism leads to a more severe manifestation and progression of albuminuria and subsequent renal failure in males compared with females. 130,133,140 Cosegregation and linkage analyses Schulz et al performed two genome-wide linkage analyses in backcross populations between MWF and a normotensive (Lewis/ FUB ) and a hypertensive (SHR/ FUB ) reference strain and identified overall 11 different QTLs on 10 rat chromosomes including RNO1 (QTL1 and QTL2), RNO4, RNO6-RNO9, RNO12, RNO15, RNO17 and RNOX, which accounted for albuminuria development in MWF (Tables 1 and 5).…”

Section: Munich Wistar Frö Mter Rat Strain Breedingmentioning

confidence: 99%

Mapping genetic determinants of kidney damage in rat models

Schulz

Kreutz

2012

Hypertens Res

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During the last two decades, significant progress in our understanding of the development of kidney diseases has been achieved by unravelling the mechanisms underlying rare familial forms of human kidney diseases. Due to the genetic heterogeneity in human populations and the complex multifactorial pathogenesis of the disease phenotypes, the dissection of the genetic basis of common chronic kidney diseases (CKD) remains a difficult task. In this regard, several inbred rat models provide valuable complementary tools to uncover the genetic basis of complex renal disease phenotypes that are related to common forms of CKD. In this review, data obtained in nine experimental rat models, including the Buffalo (BUF), Dahl salt-sensitive (SS), Fawn-hooded hypertensive (FHH), Goto-Kakizaki (GK), Lyon hypertensive (LH), Munich Wistar Frömter (MWF), Sabra hypertension-prone (SBH), spontaneously hypertensive rat (SHR) and stroke-prone spontaneously hypertensive rat (SHRSP) inbred strains, that contributed to the genetic dissection of renal disease phenotypes are presented. In this panel of inbred strains, a large number of quantitative trait loci (QTL) linked to albuminuria/proteinuria and other functional or structural kidney abnormalities could be identified by QTL mapping analysis and follow-up studies including consomic and congenic rat lines. The comprehensive exploitation of the genotype-renal phenotype associations that are inherited in this panel of rat strains is suitable for making a significant contribution to the development of an integrated approach to the systems genetics of common CKD. INTRODUCTIONCommon forms of chronic kidney diseases (CKD) represent complex disease phenotypes that are influenced by both environmental and genetic factors. [1][2][3][4][5] Both arterial hypertension and type-2 diabetes mellitus are major contributors to complex CKD, which has a high prevalence in the general human population worldwide affecting B11-15% of individuals in Europe and United States. 6-9 CKD represents as expected a major risk factor for the progression to end-stage renal disease but associates also with an increased risk of cardiovascular morbidity and mortality. 10,11 In addition to the assessment of impaired renal function or glomerular filtration rate, urinary albumin excretion rate (albuminuria) represents another important clinical marker for the evaluation of CKD and cardiovascular risk of patients. [10][11][12][13][14][15] These renal disease phenotypes are also inherited in several inbred rat strains many of which are hypertensive. 16 During the last decades, genetic mapping studies by genome-wide linkage analysis followed by fine mapping of selected quantitative trait loci (QTL) for renal disease phenotypes were reported. Subsequently, studies in consomic and congenic rats were performed to further unravel the genetics of kidney injury in inbred rat strains. For QTL confirmation, consomic strains were generated by transfer of an entire chromosome carrying a QTL from a donor strain into the disease backgro...

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Proteinuria in rats in relation to age-dependent renal changes

Cited by 62 publications

References 19 publications

Acute-Phase Proteins Before Cerebral Ischemia in Stroke-Prone Rats

Acute-Phase Proteins Before Cerebral Ischemia in Stroke-Prone Rats

Species and Strain Differences in Urinary Protein Excretion

Mapping genetic determinants of kidney damage in rat models

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