Proteoglycan biosynthesis is stimulated by D-penicillamine in chondrifying high density cell cultures

Módis, László; Hadházy, C; László, M; Kostenszky, K; Földeş, I

doi:10.1016/s0232-1513(88)80144-0

Cited by 2 publications

(2 citation statements)

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“…Although it was postulated that homocysteic acid may act as a sulfate source for phosphoadenosine phosphosulfate (PAPS), the substrate necessary for sulfoester and glycosaminoglycans synthesis, no direct confirmation of this concept has been reported [54]. The increased Ca/P ratio found on bone mineral analysis may parallel an elevation of extracellular calcium associated with a greater production of non-aggregated proteoglycans similar to those seen in vitro by others [53,55], but further study is needed. Because of the strongly polyanionic nature of their sulfate and carboxylate groups, proteoglycans have a considerable capacity to form calcium complexes, which may serve as a readily accessible source of mineral for hydroxyapatite deposition [56].…”

Section: Discussionmentioning

confidence: 99%

Chemical and biomechanical characterization of hyperhomocysteinemic bone disease in an animal model

Massé

Boskey

Ziv

et al. 2003

BMC Musculoskelet Disord

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Section: Discussionmentioning

confidence: 99%

Chemical and biomechanical characterization of hyperhomocysteinemic bone disease in an animal model

Massé

Boskey

Ziv

et al. 2003

BMC Musculoskelet Disord

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“…Primary mesenchymal cell culture system established from chondrogenic mesenchymal cells isolated from chicken limb buds is a widely used experimental model for cartilage differentiation [16][17][18][19]. This high density cell (HDC) culture system represents a unusual kind of primary differentiating cells, in which a high concentration of cell suspension (15 million cells/mL) is required to initiate differentiation [20].…”

Section: Introductionmentioning

confidence: 99%

Optimalized transient transfection of chondrogenic primary cell cultures

et al. 2010

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Abstract:We aimed to find a transfection method which provides high efficiency with minimal cytotoxic and/or apoptotic effects for gene transfer into multilayer primary chondrogenic cell cultures. The pEGFP-C1 plasmid was introduced into the cell culture and the efficiency of transformation quantified by GFP fluorescence; the resulting nucleofection was effective but resulted in severe apoptosis. Two liposomal reagents designed to allow transfection into adherent cells did not deliver the plasmids sufficiently and cartilage formation did not occur. In addition, a third liposomal compound, recommended for transfection into either adherent or suspension cell cultures, lead to acceptable transfection efficiency but no cartilage formation. When an amphiphilic reagent was used however, there was acceptable transfection efficiency as well as cartilage formation. The viability of the cells which were transfected using the amphiphilic reagent remained unaffected but proliferation was severely diminished, particularly in the presence of GFP. In addition, the amount of cartilage decreased when GFP was expressed, despite unchanged levels of mRNAs of sox9 and aggrecan core protein, factors reflecting on the efficiency of chondrogenesis. Overexpression of both the constitutively active delta and gamma isoforms of catalytic subunit of calcineurin, a protein phosphatase described as a positive regulator of chondrogenesis, decreased protein level of Sox9 and subsequent cartilage formation. In conclusion, we found that amphiphilic reagent applied prior to the adhesion of cells provides a useful means to transfer plasmids to primary differentiating chondrogenic cells.

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Proteoglycan biosynthesis is stimulated by D-penicillamine in chondrifying high density cell cultures

Cited by 2 publications

References 39 publications

Chemical and biomechanical characterization of hyperhomocysteinemic bone disease in an animal model

Chemical and biomechanical characterization of hyperhomocysteinemic bone disease in an animal model

Optimalized transient transfection of chondrogenic primary cell cultures

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