Proteoglycan Depletion and Magnetic Resonance Parameters of Articular Cartilage

Toffanin, Renato; Mlynarik, Vladimir; Russo, Stefano Alberto; Szomolányi, Pavol; Piras, Alessandro; Vittur, Franco

doi:10.1006/abbi.2001.2338

Cited by 54 publications

(42 citation statements)

References 20 publications

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“…For many of these studies, cartilage plugs were detached from the bone. Some studies concentrated on the influence of PG depletion on proton magnetic resonance parameters, such as T,, T1 and T1, relaxation times, diffusion constant, and magnetization transfer contrast [6,41]. The relaxation rates were also studied as a function of osmotic pressure [2 11.…”

Section: Introductionmentioning

confidence: 99%

The effect of detachment of the articular cartilage from its calcified zone on the cartilage microstructure, assessed by ²H‐spectroscopic double quantum filtered MRI

Keinan‐Adamsky¹,

Shinar²

2005

Journal Orthopaedic Research

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Most studies on articular cartilage properties have been conducted after detachment of the cartilage from the bone. In the present work we investigated the effect of detachment on collagen fiber architecture. We used one-dimensional 2H double quantum filtered MRI on cartilage bone plugs equilibrated in deuterated saline. The quadrupolar splittings observed in the different zones were related to the degree of order and the density of the collagen fibers. The method is non-destructive, allowing for measurements on the same plug without the need for fixation, dehydration, sectioning and decalcification. Detachment of the radial from the calcified zone resulted in swelling of the cartilage plug in physiological saline and a concomitant decrease in the quadrupolar splitting. The effect of mechanical pressure on the 'H quadrupolar splittings for the detached cartilage and for the calcified zone-bone plugs were compared with those of the same zones in the intact cartilage-bone plug. The splitting in the radial zone of the detached cartilage collapsed at much smaller loads compared to the intact cartilage-bone plug. The effect of the load on the size of the cartilage was also greater for the detached plug. These results indicate that anchoring of the cartilage to the bone through the calcified zone plays an important role in retaining the order of the collagen fibers. The water 2H quadrupolar splitting in intact and proteoglycan-depleted cartilage was the same, indicating that the proteoglycans d o not contribute to the ordering of the collagen fibers.

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Section: Introductionmentioning

confidence: 99%

The effect of detachment of the articular cartilage from its calcified zone on the cartilage microstructure, assessed by ²H‐spectroscopic double quantum filtered MRI

Keinan‐Adamsky¹,

Shinar²

2005

Journal Orthopaedic Research

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“…As has been demonstrated in vitro (14) and in vivo (15), T 1 relaxation in delayed Gd-DTPA 2 -enhanced MRI of cartilage (dGEMRIC) can be considered the method of choice for detecting proteoglycan depletion in articular cartilage. A recent study (16) showed that only T 1 relaxation times and ADC of articular cartilage varied significantly when cartilage proteoglycan content was reduced by treatment with guanidine and the specimens were subsequently conditioned in saline.…”

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confidence: 99%

Investigation of apparent diffusion constant as an indicator of early degenerative disease in articular cartilage

Mlynárik

Sulzbacher

Bittšanský

et al. 2003

Magnetic Resonance Imaging

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Purpose: To investigate the apparent diffusion constant (ADC) as a prospective magnetic resonance imaging (MRI) marker of early degeneration in articular cartilage. Materials and Methods:Early degenerative changes were studied using in vitro MRI on cartilage-bone specimens excised from human femoral condyles. The loss of proteoglycans developed in vivo due to a degenerative process was compared with a gadolinium diethylenetriamine pentaacetate anion (Gd-DTPA 2-) enhanced decrease of T 1 relaxation times, and with an increase of ADCs and T 2 relaxation times.Results: Contrast enhanced T 1 values decreased and the diffusion constants increased in cartilage regions with depleted proteoglycans. The relative changes in diffusion constants were smaller than those of Gd-DTPA 2-enhanced T 1 , and in some proteoglycan-depleted regions no changes in the diffusion constants were detected. T 2 relaxation times showed considerable spatial variability that did not correlate with proteoglycan concentration. Conclusion:In contrast to Gd-DTPA 2-enhanced T 1 , which reflects changes in chemical composition, diffusion constants may reflect structural degradation of the cartilage matrix.

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“…[5][6][7]9,[12][13][14][15] In particular, PG content decreases before the loss of denatured collagen, but morphologic changes are not seen in the early stage of osteoarthritis (OA). [4][5][6][7] Although the early denaturalization of articular cartilage can be depicted by T 1 r mapping as well as T 2 mapping and dGEM-RIC, [10][11][12][13][14][15][16] its depiction requires an elongated scanning time. Previous reports in the literature used 4 or 5 diŠerent T 1 r-prepared images with diŠerent TSLs for calculation, which required more than 12 min of scanning time.…”

Section: Discussionmentioning

confidence: 99%

“…1,2 As well, MR imaging can be used to assess the biochemical properties of articular cartilage by quantitative imaging approaches, such as sodium MR imaging, delayed gadolinium-enhanced MR imaging of cartilage (dGEMRIC), magnetization transfer imaging, T 2 mapping, diŠusion-weighted imaging, and T 1 r mapping. [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17] These approaches have been used to allow histological analysis of cellular morphology, proteoglycan (PG) concentrations, collagenˆber size, and orientation of hyaline cartilage. Sodium MR imaging and dGEMRIC have been employed to quantify the changes in PG depletion in articular cartilage.…”

Section: Introductionmentioning

confidence: 99%

A Simplified Method of T1ρ Mapping in Clinical Assessment of Knee Joint

et al. 2010

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Purpose: We assessed the clinical utility of our proposed simpliˆed method for T 1 r mapping calculations.Materials and Methods: Ten healthy subjects underwent scanning on a 3-tesla magnetic resonance system using an 8-channel phased-array coil. For each subject, we obtained sagittal T 1 r-prepared images using 5 diŠerent time of spin-lock pulses (TSL＝1, 20, 40, 60, and 80 ms), produced conventional T 1 r maps (cT 1 r maps) using all TSLs, and recomputed our proposed simpliˆed T 1 r maps (sT 1 r maps) using a decreasing number of TSLs (from 4 to 2). We then investigated the diŠerences and correlations in T 1 r values of the tissues obtained using diŠerent numbers of spin-lock times.Results: There was a strong positive correlation (single measure intraclass correlation coe‹cient＝0.948; 95z conˆdence interval＝0.911 to 0.970) in T 1 r values of tissues between the cT 1 r and sT 1 r [1, 80] maps. The 2 maps were comparable, though there was a small diŠerence in T 1 r value between the two. The total scan time to acquire the data from 5 spin-lock times was 16 min 15 s. Similarity of the T 1 r [1, 80] map with the conventional approach reduced scan time by 60z, to 6 min 30 s.Conclusion: The clinical relevance of our proposed simpliˆed method is potentially similar to that of the conventional method, and our method requires a shorter examination time and generally preserves the reliability of the T 1 r relaxation time of the tissues.

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Proteoglycan Depletion and Magnetic Resonance Parameters of Articular Cartilage

Cited by 54 publications

References 20 publications

The effect of detachment of the articular cartilage from its calcified zone on the cartilage microstructure, assessed by ²H‐spectroscopic double quantum filtered MRI

The effect of detachment of the articular cartilage from its calcified zone on the cartilage microstructure, assessed by ²H‐spectroscopic double quantum filtered MRI

Investigation of apparent diffusion constant as an indicator of early degenerative disease in articular cartilage

A Simplified Method of T1ρ Mapping in Clinical Assessment of Knee Joint

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Proteoglycan Depletion and Magnetic Resonance Parameters of Articular Cartilage

Cited by 54 publications

References 20 publications

The effect of detachment of the articular cartilage from its calcified zone on the cartilage microstructure, assessed by 2H‐spectroscopic double quantum filtered MRI

The effect of detachment of the articular cartilage from its calcified zone on the cartilage microstructure, assessed by 2H‐spectroscopic double quantum filtered MRI

Investigation of apparent diffusion constant as an indicator of early degenerative disease in articular cartilage

A Simplified Method of T1ρ Mapping in Clinical Assessment of Knee Joint

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The effect of detachment of the articular cartilage from its calcified zone on the cartilage microstructure, assessed by ²H‐spectroscopic double quantum filtered MRI

The effect of detachment of the articular cartilage from its calcified zone on the cartilage microstructure, assessed by ²H‐spectroscopic double quantum filtered MRI