Proteolysis of Thyroglobulin by Thyroid Enzymes

McQuillan, Mary T.; Mathews, Justin; Trikojus, V. M.

doi:10.1038/192333a0

Cited by 34 publications

(6 citation statements)

References 11 publications

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“…Fractions B and C had amino acid contents, hexose (4%) and sialic acid (1.2%) indistinguishable from those of Tg (see [ 12,131). (Polyacrylamide gel electrophoretic analysis of freshly prepared Al in R buffer has shown Al to be heterogeneous.)…”

Section: Resultsmentioning

confidence: 99%

The nature of the breakdown of thyroglobulin

Impiombato

Pitt‐Rivers

1971

FEBS Letters

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Section: Resultsmentioning

confidence: 99%

The nature of the breakdown of thyroglobulin

Impiombato

Pitt‐Rivers

1971

FEBS Letters

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“…-The activity of our protease solution towards labelled rat thyroid was reduced fourfold when the incubation was carried out at pH 5-6. This may have been due to loss during preparation -of a protease fraction demonstrated by Haddad & Rall (1960) and by McQuillan, Mathews & Trikojus (1961) to be active at this pH. No attempt was made to inactivate the ratthyroid protease in the glands themselves, or to separate it from rat thyroglobulin.…”

Section: Autoly8i8 Of Thyroid Protein During Dialy8i8mentioning

confidence: 99%

The free iodotyrosines of the rat thyroid gland

Pitt‐Rivers¹,

Cavalieri²

1963

Biochemical Journal

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“…It is likely that thyroglobulin is hydrolysed mainly by the thyroid mast-cell enzyme at pH 8-0 or 8-5 in vitro (Pastan & Almqvist, 1966a,b), rather than by the thyroidal proteases and peptidases that McQuillan, Mathews & Trikojus (1961) showed to have acid pH optima.…”

Section: Discussionmentioning

confidence: 99%

Hydrolysis of 125I-labelled thyroglobulin by pancreatin, Pronase and pepsin

Malan

1968

Biochemical Journal

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1. Pancreatin or Pronase hydrolysis of partially purified heat-denatured (125)I-labelled thyroglobulin does not release all the iodoamino acids from peptide linkage. 2. The addition of thyroidal proteases is essential to obtain maximum hydrolysis of thyroglobulin. 3. Pronase plus a fresh non-radioactive thyroid homogenate gives the best hydrolysis results, but peptide ;mapping' shows that about 10% of the radioactivity remains in the form of peptides. 4. These peptides have approximately the same R(F) as the iodotyrosines on one-dimensional chromatograms developed in butan-1-ol-2n-acetic acid (1:1, v/v). 5. Peptic hydrolysis of thyroglobulin releases virtually all of the iodothyronines but little of the iodotyrosines, in contrast with the action of pancreatin. 6. Hydrolysis of thyroglobulin with pepsin followed by Pronase plus a source of thyroidal proteases is satisfactory, but the results are not superior to those obtained by using Pronase plus a source of thyroidal proteases.

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Proteolysis of Thyroglobulin by Thyroid Enzymes

Cited by 34 publications

References 11 publications

The nature of the breakdown of thyroglobulin

The nature of the breakdown of thyroglobulin

The free iodotyrosines of the rat thyroid gland

Hydrolysis of 125I-labelled thyroglobulin by pancreatin, Pronase and pepsin

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