Proteolytic activity and immunogenicity of oral bromelain within the gastrointestinal tract of mice

Hale, Laura P.

doi:10.1016/j.intimp.2003.12.010

Cited by 78 publications

(59 citation statements)

References 25 publications

(27 reference statements)

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“…One biopsy of each set was treated with 1 mg/ml bromelain (catalog #B-4882; Sigma-Aldrich Chemical Co., St. Louis, MO) in RPMI + 1X pen/strep (media) for 1 hour at 37°C while the other was treated with media alone. These treatment conditions have previously been shown to be sufficient to remove bromelain-sensitive molecules from a variety of different cell types (15,16,21). All biopsies were treated using aliquots derived from the same lot of bromelain.…”

Section: Culture Of Colon Biopsies and Cytokine Assaysmentioning

confidence: 99%

“…Treatment with proteolytically active bromelain affects T cell expression of mRNAs encoding IL-2, IL-4, and interferon (IFN)-γ and inhibits ERK-2-mediated signal transduction in leukocyte and colon epithelial cell lines in vitro (19,20). We recently showed that oral bromelain can retain sufficient proteolytic activity within the gastrointestinal tract of mice to remove bromelain-sensitive molecules from intestinal epithelial cells and macrophages (21). Furthermore, treatment with oral bromelain decreases the incidence of spontaneous colitis and the severity of both spontaneous and established colitis in C57BL/6 IL-10 −/− mice (22).…”

Section: Introductionmentioning

confidence: 99%

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Bromelain treatment decreases secretion of pro-inflammatory cytokines and chemokines by colon biopsies in vitro

Onken

Greer

Calingaert

et al. 2008

Clinical Immunology

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Oral bromelain has been anecdotally reported to decrease inflammation in ulcerative colitis (UC). Proteolytically active bromelain is known to decrease expression of mRNAs encoding proinflammatory cytokines by human leukocytes in vitro. To assess the effect of bromelain on mucosal secretion of cytokines in inflammatory bowel disease (IBD), endoscopic colon biopsies from patients with UC, Crohn's disease (CD), and non-IBD controls were treated in vitro with bromelain or media, then cultured. Secretion of pro-inflammatory cytokines and chemokines was measured. Significant increases in granulocyte colony stimulating factor (G-CSF), interferon (IFN)-γ, interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF) were detected in the media from actively inflamed areas in UC and CD as compared with non-inflamed IBD tissue and non-IBD controls. In vitro bromelain treatment decreased secretion of G-CSF, granulocyte-macrophage colony stimulating factor (GM-CSF), IFN-γ, CCL4/macrophage inhibitory protein (MIP)-1β, and TNF by inflamed tissue in IBD. Bromelain may be a novel therapy for IBD.

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Section: Culture Of Colon Biopsies and Cytokine Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bromelain treatment decreases secretion of pro-inflammatory cytokines and chemokines by colon biopsies in vitro

Onken

Greer

Calingaert

et al. 2008

Clinical Immunology

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“…Experimental evidence suggests that bromelain's action as an anti-inflammatory is mediated via the following factors: (i) by increasing serum fibrinolytic activity (13), reducing plasma fibrinogen levels (14) and decreasing bradykinin levels (which results in reduced vascular permeability) and hence reducing oedema and pain (15); (ii) by mediating prostaglandin levels (by decreasing levels of PGE 2 and thromboxane A 2 ); and (iii) through modulation of certain immune cell surface adhesion molecules (16)(17)(18)(19)(20), which play a role in the pathogenesis of arthritis (21). However, many of these studies are of poor quality and further data is needed to clarify definitive mechanisms of its action.…”

Section: Mechanism Of Actionmentioning

confidence: 99%

Bromelain as a Treatment for Osteoarthritis: a Review of Clinical Studies

Brien

2004

Evidence-based Complementary and Alternative Medicine

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Bromelain, an extract from the pineapple plant, has been demonstrated to show anti-inflammatory and analgesic properties and may provide a safer alternative or adjunctive treatment for osteoarthritis. All previous trials, which have been uncontrolled or comparative studies, indicate its potential use for the treatment of osteoarthritis. This paper reviews the mechanism of its putative therapeutic actions, those clinical trials that have assessed its use in osteoarthritis to date, as well as considering the safety implications of this supplement for osteoarthritis and reviewing the evidence to date regarding the dosage for treating this condition. The data available at present indicate the need for trials to establish the efficacy and optimum dosage for bromelain and the need for adequate prospective adverse event monitoring in such chronic conditions as osteoarthritis.

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“…Hale et al [5] also reported that proteolytic activity is required for the therapeutic effect of bromelain. Bromelain exhibits therapeutic and pharmacological effects such as anti-inflammatory tumour growth modulation and aids in digestion [6] [7].…”

Section: Introductionmentioning

confidence: 99%

Effect of Combining Ultrasound and Mild Heat Treatment on Physicochemical, Nutritional Quality and Microbiological Properties of Pineapple Juice

Lagnika¹,

Adjovi²,

Lagnika³

et al. 2017

FNS

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Increasing consumer awareness regarding the health benefits of different nutrients in food has led to the requirement of assessing the effect of food processing approaches on the quality attributes. The present work focuses on understanding the effects of ultrasound (US) processing, mild heat pasteurization (65˚C for 15 min), thermal pasteurization (80˚C for 15 min) and their combination on physicochemical, microbiological properties and nutritional quality of pineapple juice through 60 days of storage at room temperature. Ultrasound treatment showed significantly lower browning degree. Ultrasound followed by ultrasound combined with mild heat pasteurization (UMP) treatments was effective in retaining the total phenolic content of pineapple juice as compared to the thermal treatment or the untreated juice sample at room temperature during 60 days of storage. Thermal pasteurization (TP) followed by ultrasound combined with mild heat pasteurization (UMP) and ultrasound (US) treatment, in increasing order, was found to be effective in delaying microbial growth in pineapple juice. This study demonstrates that ultrasound combined with mild heat pasteurization treatments could be able to effectively inactivate the microorganisms and pectin methylesterase in pineapple juice whilst preserving relatively high amount of phenols.

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Proteolytic activity and immunogenicity of oral bromelain within the gastrointestinal tract of mice

Cited by 78 publications

References 25 publications

Bromelain treatment decreases secretion of pro-inflammatory cytokines and chemokines by colon biopsies in vitro

Bromelain treatment decreases secretion of pro-inflammatory cytokines and chemokines by colon biopsies in vitro

Bromelain as a Treatment for Osteoarthritis: a Review of Clinical Studies

Effect of Combining Ultrasound and Mild Heat Treatment on Physicochemical, Nutritional Quality and Microbiological Properties of Pineapple Juice

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