Proteolytic enzymes in fish development and the importance of dietary enzymes

Lauff, M.; Hofer, R.

doi:10.1016/0044-8486(84)90298-9

Cited by 222 publications

(139 citation statements)

References 12 publications

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“…A change in the pattern of trypsin isozymes also occurs during coregonid development, suggesting that the larval form of the enzyme disappears. Lauff and Hofer (1984) conclude that external enzymes obtained from zooplankton may contribute 70-80 % of the total enzyme activity in the fish digestive tract, confirming the earlier results of Dabrowski and Glogowski (1977).…”

Section: Introductionsupporting

confidence: 81%

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The feeding of fish larvae : present « state of the art » and perspectives

Dąbrowski¹

1984

Reprod. Nutr. Dévelop.

241

149

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Section: Introductionsupporting

confidence: 81%

“…Trypsin, chymotrypsin and aminopeptidase activities also undergo ontogenic changes in coregonid larvae (Lauff and Hofer, 1984). Tryptic activity in these larvae is only slightly lower than that of rainbow trout, whereas chymotrypsin activity is much lower in the former species up to day 50 of life.…”

Section: Introductionmentioning

confidence: 97%

The feeding of fish larvae : present « state of the art » and perspectives

Dąbrowski¹

1984

Reprod. Nutr. Dévelop.

241

149

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“…Some authors have suggested that larvae utilize enzymes from their prey to facilitate the process of digestion until the larval alimentary system is fully differentiated and developed [61][62][63][64]. The authors estimated that 40-80 % of the enzymatic activity was donated by the live food organisms.…”

Section: Discussionmentioning

confidence: 99%

Digestive Enzymes in Larvae and Juveniles of Farmed Sharpsnout Seabream (Diplodus puntazzo) (Cetti, 1777)

Savona¹

2011

TOMBJ

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Abstract:The ontogenetic development and pattern of main digestive enzyme activities (proteases, carbohydrases and lipases) were investigated in farmed sharpsnout seabream Diplodus puntazzo larvae and juveniles, during the passage from the larval stage to adult (from 21 to 277 days after hatching -DAH). Larvae showed exponential growth, thus confirming that Rotifers and Artemia nauplii are suitable preys for early feeding of D. puntazzo larvae.Pepsin activity was low in the larvae, but it increased afterwards in relation with stomach development. In juveniles, levels of specific activity of trypsin, chymotrypsin and elastase were lower compared to that recorded in larvae. A decrease in the activity of the alkaline proteases could be related to an increase in the functionality of stomach. Qualitative and quantitative assay of glycosidases ( -amylase, cellulase and maltase) showed high glycosidase activity, indicating that D. puntazzo is capable of digesting carbohydrates in its diet. Levels of lipase activity in juveniles were higher than those found in larvae. Our results didn't show an inductive character of lipases, since in juveniles the activity levels were almost constant even if the fat content of compound diet varied (from 7.0 % to 20 %).The rapid changes in the digestive activities of D. puntazzo larvae supported the existence of well established induction and regulation digestive mechanisms. Present results also confirm that D. puntazzo larvae have complete digestive enzymatic equipment that increased during larval ontogeny and enable larvae to digest and absorb nutrients contained in live preys once exogenous feeding occurred. The enzymatic pattern is correlated with omnivorous feeding habits of the species. Moreover, the patterns of activity of the main digestive enzymes of D. puntazzo larvae and juveniles indicated the early functional development of their digestive system and suggested this species as a good candidate for Mediterranean aquaculture using the compound diet.

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“…However, enzyme activity in fish larvae is age-dependent and modulated by diet composition (Cahu and Zambonino-Infante, 2001). Varying responses in terms of proteolytic activity has been observed when fish larvae are fed diets having different C:N ratios and an increasing amount of prey in the gut stimulates the total proteolytic activity (Lauff and Hofer, 1984). A better knowledge of serine protease regulation at the transcriptional and protein level is crucial to advance in the design of formulated diets that can replace live feeds during larval culture.…”

Section: Effect Of Feeding Regime On Trypsinogen Gene Expression and mentioning

confidence: 99%