Proteome analysis and differential expression by JA driven elicitation in Andrographis paniculata (Burm. f.) Wall. ex Nees using Q-TOF–LC–MS/MS

Bindu, Byreddi Bhavani Venkata; Srinath, Mote; Shailaja, Aayeti; Giri, Charu Chandra

doi:10.1007/s11240-019-01741-0

Cited by 11 publications

(3 citation statements)

References 59 publications

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“…Our present finding may be attributed to the JA mechanism of action in inducing genes and transcription factors in adventitious root cultures of A. paniculata [38,47]. Such observation further highlighted the combo action of in vitro adventitious root cultures, and JA elicitation can contribute towards understanding the gene expression pattern and biosynthesis of andrographolide and other DLs.…”

Section: Discussionsupporting

confidence: 70%

“…Despite the intensive study of genes and proteins contributing for diterpene biosynthesis in A. paniculata plant, a meager information is available for the isolation and characterization of the MEP pathway genes. However, in our laboratory, we have ongoing comprehensive research programs to decipher both MVA and MEP pathway genes using genomic and proteomic approaches [34,38]. In the present study, we have isolated, cloned, and carried out in silico functional characterization of DXS gene from A. paniculata for the first time.…”

Section: Discussionmentioning

confidence: 98%

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Molecular Cloning and Differential Gene Expression Analysis of 1-Deoxy-D-xylulose 5-Phosphate Synthase (DXS) in Andrographis paniculata (Burm. f) Nees

et al. 2020

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Andrographis paniculata 1-deoxy-D-xylulose-5-phosphate synthase (ApDXS) gene (GenBank Accession No MG271749.1) was isolated and cloned from leaves for the first time. Expression of ApDXS gene was carried out in Escherichia coli Rosetta cells. Tissue-specific ApDXS gene expression by quantitative RT-PCR (qRT-PCR) revealed maximum fold expression in the leaves followed by stem and roots. Further, the differential gene expression profile of Jasmonic acid (JA)-elicited in vitro adventitious root cultures showed enhanced ApDXS expression compared to untreated control cultures. A. paniculata 3-hydroxy-3-methylglutaryl-coenzyme A reductase (ApHMGR) gene expression was also studied where it was up-regulated by JA elicitation but showed lower expression compared to ApDXS. The highest expression of both genes was found at 25 µm JA elicitation followed by 50 µm. HPLC data indicated that the transcription levels were correlated with increased andrographolide accumulation. The peak level of andrographolide accumulation was recorded at 25 μM JA (9.38-fold) followed by 50 µM JA (7.58-fold) in elicitation treatments. The in silico generated ApDXS 3D model revealed 98% expected amino acid residues in the favored and 2% in the allowed regions of the Ramachandran plot with 92% structural reliability. Further, prediction of conserved domains and essential amino acids [Arg (249, 252, 255), Asn (307) and Ser (247)] involved in ligand/ inhibitor binding was carried out by in silico docking studies. Our present findings will generate genomic information and provide a blueprint for future studies of ApDXS and its role in diterpenoid biosynthesis in A. paniculata.

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Section: Discussionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 98%

Molecular Cloning and Differential Gene Expression Analysis of 1-Deoxy-D-xylulose 5-Phosphate Synthase (DXS) in Andrographis paniculata (Burm. f) Nees

et al. 2020

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“…Leaves of A. paniculata (AP) [24][25][26][27] were collected from Vellingiri hills, Coimbatore. The collected leaf sample was washed and shade dried for 14 days in an oxygen atmosphere.…”

Section: Methodsmentioning

confidence: 99%

Andrographis paniculata absorbed ZnO nanofibers as a potential antimicrobial agent for biomedical applications

Brindha

Kandeeban²,

Kamal³

et al. 2021

Adv. Nat. Sci: Nanosci. Nanotechnol.

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The present study focuses on developing antimicrobial nanofibers with superhydrophobicity and insect repellent properties for wound dressing and biomedical applications. For the first time, Andrographis paniculata leaf extract was prepared and incorporated into ZnO nanofibers (AP-ZnO). The antifungal and antibacterial activities of AP-ZnO were tested using the agar plate method. Amid the bacteria and fungus strain tested, the prepared sample exhibited a greater efficiency against Staphylococcus aureus and Trichophyton rubrum respectively. AP-ZnO showed the highest larvicidal activity (100 ± 0.2) against Aedes aegypti, proving its insect repellent characteristics. The in vivo toxicity studies of AP-ZnO (100 μg ml−1) tested on Danio rerio ensure the biocompatibility of the prepared sample with maximum toxicity of 21.1% after 72 h, which is lower than commercial Prallethrin. Moreover, AP-ZnO-chitosan coated cotton fabric showed higher durability with contact angle (θ ∼ 151°) and was suggested to be used for self-cleaning applications in the biomedical sector. The examined results confirmed that AP-ZnO possesses different medicinal characteristics suitable for biomedical and pharmaceutical applications.

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Recent Trends in Elicitation and Secondary Metabolic Pathway Analysis in Medicinal Plants: An Integrated Transcriptomics and Proteomics Approach

Giri

2022

Medicinal and Aromatic Plants of the World

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Proteome analysis and differential expression by JA driven elicitation in Andrographis paniculata (Burm. f.) Wall. ex Nees using Q-TOF–LC–MS/MS

Cited by 11 publications

References 59 publications

Molecular Cloning and Differential Gene Expression Analysis of 1-Deoxy-D-xylulose 5-Phosphate Synthase (DXS) in Andrographis paniculata (Burm. f) Nees

Molecular Cloning and Differential Gene Expression Analysis of 1-Deoxy-D-xylulose 5-Phosphate Synthase (DXS) in Andrographis paniculata (Burm. f) Nees

Andrographis paniculata absorbed ZnO nanofibers as a potential antimicrobial agent for biomedical applications

Recent Trends in Elicitation and Secondary Metabolic Pathway Analysis in Medicinal Plants: An Integrated Transcriptomics and Proteomics Approach

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