Proteome Analysis and Tissue Microarray for Profiling Protein Markers Associated with Lymph Node Metastasis in Colorectal Cancer

Pei, Haiping; Zhu, Hong; Zeng, Shan; Li, Yixiong; Yang, Huixiang; Liang-fang, Shen; Jia, Chen; Zeng, Liang; Fan, Jianghong; Li, O Xiaogang; Gong, Yuewen; Shen, Hong

doi:10.1021/pr060644r

Cited by 90 publications

(96 citation statements)

References 20 publications

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“…To some extent, this result is consistent with the data reported by other groups, who listed several proteins involved in protein synthesis and folding (heat shock proteins), cellular reorganization and cytoskeleton (actin, tubulin), intracellular calciumbinding protein (calgranulin), and cell communication and signal transduction (annexin) in the proteomic profiles of colorectal cancer cell line and tissue. [27][28][29][30] However, there are still some different proteins between our data and other researchers' reports. 31,32 First, we consider that two-dimensional electrophoresis and MALDI-TOF MS-based PMF analysis for human tissue is more complex than that for cell line.…”

Section: Discussionmentioning

confidence: 56%

Transgelin as a suppressor is associated with poor prognosis in colorectal carcinoma patients

et al. 2009

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We performed comparative proteomic analysis of colorectal cancer to investigate potential target proteins correlated with carcinogenesis and prognosis. Among them, transgelin, a 22 kDa protein also called SM22, was identified as a novel tumor suppressor protein, but little is known about this protein in tumors so far. A remarkable reduced expression of transgelin was found in colorectal cancer samples compared with normal colorectal mucosa. The effect of 5-aza-2 0 -deoxycytidine as a demethylation agent would obviously restore the original expression level of transgelin, implicating DNA hypermethylation of transgelin is important in the regulation of transgelin transcription in colorectal cancer. As a control, the investigation at cell line level confirms that transgelin protein comes from epithelium but not mesenchymal cells. Further, immunohistochemical staining for transgelin was performed on paraffin sections of 62 and 126 cases of normal colorectal mucosa and colorectal cancer specimens, respectively. As compared to normal colorectal tissue, we observed a significantly lower transgelin expression in colorectal cancer samples (Po0.001). Survival analysis demonstrated that patients without transgelin expression had shorter overall survival, whereas patients with transgelin expression had better survival (P ¼ 0.006). Multivariate analysis showed that negative transgelin expression was an independent prognostic indicator for patient's survival. Our results suggest that transgelin as a suppressor may serve as important biomarker of malignancy. Loss of transgelin involves gene promoter hypermethylation and is closely associated with poor overall survival in colorectal cancer patients.

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Section: Discussionmentioning

confidence: 56%

Transgelin as a suppressor is associated with poor prognosis in colorectal carcinoma patients

et al. 2009

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“…To further analyze the degree of interstitial collagen deposition, Masson's trichrome-stained sections were graded as that in a previous report (9). Following an antigen retrieval by 6.5 mM citrate buffer (pH 6.0) pressure cooking and endogenous peroxidase activity blockage with 3% hydrogen peroxide in methanol for 20 min at room temperature, DAB immunohistochemistry staining was processed with the DAKO EnVision System (37). In brief, the slides were incubated with the primary antibody against collagen ␣ 1(I) or ␣-SMA overnight at 4°C, and then treated with a pepsin-based antigen retrieval technique.…”

Section: Methodsmentioning

confidence: 99%

Fluorofenidone attenuates hepatic fibrosis by suppressing the proliferation and activation of hepatic stellate cells

Yang

Wang

et al. 2014

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…Briefly, following a brief proteolytic digestion and a peroxidase blocking of tissue slides, the slides were incubated with the primary antibody against the respective target protein at a dilution of 1:500 overnight at 40°C. After PBS washing, peroxidase labeled polymer and substrate-chromogen were then employed in order to visualize the staining of the protein of interest [27]. Immunohistochemical staining was performed at the pathology department of the National Hepatobiliary and Enteric Surgery Research Center, Ministry of Health, China.…”

Section: Methodsmentioning

confidence: 99%

Expression Levels of Insulin-Like Growth Factor-1 and Multidrug Resistance-Associated Protein-1 Indicate Poor Prognosis in Patients with Gastric Cancer

Chen

et al. 2009

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Background/Objectives: Insulin-like growth factor-1 receptor (IGF-1R) displays a key role in tumor transformation and metastasis. It also makes tumor cells more resistant to chemotherapy. The aim of this study was to investigate the correlation between IGF-1R and multidrug resistance-associated protein-1 (MRP-1), and the clinical significance of their expression in gastric carcinoma (GC). Methods: IGF-1R and MRP-1 expressed in 113 specimens were detected by immunohistochemistry. The correlation between IGF-1R and MRP-1 expression was determined. In addition, the association of their expression with clinicopathological features and survival data of GC was also analyzed. Results: IGF-1R (75.2%) and MRP-1 (69.0%) were frequently expressed in GC. IGF-1R was associated with tumor size, quantity of stroma, depth of wall invasion, lymph node metastasis, TNM stages and differentiation status of GC (p < 0.05). MRP-1 was associated with tumor size, quantity of stroma, lymph node metastasis, distant metastasis and TNM stages (p < 0.05). IGF-1R over-expression positively correlated with MRP-1 over-expression (rp = 0.39, p < 0.01). IGF-1R and MPR-1 over-expression were correlated with poor prognosis of GC (p < 0.01). 102 patients receiving adjuvant FOLFOX-4 chemotherapy who co-expressed IGF-1R/MRP-1 had poor prognosis (p < 0.05). Conclusions: The levels of co-expression of IGF-1R/MRP-1 in GC may predict the therapeutic effect of chemotherapy and prognosis of GC.

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Proteome Analysis and Tissue Microarray for Profiling Protein Markers Associated with Lymph Node Metastasis in Colorectal Cancer

Cited by 90 publications

References 20 publications

Transgelin as a suppressor is associated with poor prognosis in colorectal carcinoma patients

Transgelin as a suppressor is associated with poor prognosis in colorectal carcinoma patients

Fluorofenidone attenuates hepatic fibrosis by suppressing the proliferation and activation of hepatic stellate cells

Expression Levels of Insulin-Like Growth Factor-1 and Multidrug Resistance-Associated Protein-1 Indicate Poor Prognosis in Patients with Gastric Cancer

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