Proteome analysis of Duck Tembusu virus (DTMUV)‐infected BHK‐21 cells

Sun, Xin; Wang, Shengyu; Lin, Xian; Zhao, Lianzhong; Zhang, Dan; Yi, Chenyang; Sun, Xiaomei; Chen, Huanchun; Jin, Meilin

doi:10.1002/pmic.201700033

Cited by 15 publications

(14 citation statements)

References 63 publications

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“…The JEV standard virus (Beijing strain) was provided by the Animal Vector‐Borne Disease Research Group, Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University. The DK/TH/CU‐1 and JEV Beijing strain were propagated in baby hamster kidney (BHK‐21) cells as described previously (Sun et al., ; Teng et al., ). The viruses were harvested, clarified by centrifugation and the 50% tissue culture infectious doses (TCID 50 ) of viruses were calculated by the Reed and Muench method (Reed & Muench, ).…”

Section: Methodsmentioning

confidence: 99%

Serological evidence of duck Tembusu virus infection in free-grazing ducks, Thailand

Tunterak

Prakairungnamthip

Ninvilai

et al. 2018

Transbound Emerg Dis

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Duck Tembusu virus (DTMUV) has been reported in ducks raised in farming system since its emergence in 2010. No information is available on DTMUV infection in free-grazing ducks, which are commonly raised and widespread in several Asian countries. To determine the presence of DTMUV infection in free-grazing ducks in Thailand, retrospective serum samples collected from 1,000 free-grazing ducks during 2008-2015 were tested for DTMUV infection. Our result showed that 91 (9.10%) were positive for DTMUV neutralizing antibodies and DTMUV seropositive ducks have been detected in Thailand since 2008. To further investigate the seroprevalence and geographic distribution of DTMUV infection in free-grazing ducks in Thailand, a cross-sectional serological survey of DTMUV was conducted in 2016. Of 1,200 free-grazing ducks in the 60 flocks from 20 provinces located in the major free-grazing duck raising areas of Thailand, 365 (30.42%) were positive for DTMUV neutralizing antibodies and 56 flocks (93.33%) had at least one DTMUV seropositive duck. Additionally, DTMUV seropositive ducks were observed in all provinces tested. In conclusion, our data demonstrated the presence of DTMUV infection in free-grazing ducks since 2008 and widespread DTMUV infection in free-grazing ducks in Thailand with a relatively high seroprevalence. These findings suggest the potential role of free-grazing ducks in the dissemination of DTMUV and highlight the necessity of systemic DTMUV surveillance in free-grazing ducks in addition to farm ducks for early detection, prevention, and control of this emerging disease.

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Section: Methodsmentioning

confidence: 99%

Serological evidence of duck Tembusu virus infection in free-grazing ducks, Thailand

Tunterak

Prakairungnamthip

Ninvilai

et al. 2018

Transbound Emerg Dis

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“…Recently, several studies demonstrated the expression changes of several DDX proteins after DTMUV infection. Sun et al reported that DDX3X and DDX5 were significantly decreased in DTMUV-infected BHK-21 cells, and they found that DDX3X overexpression could inhibit DTMUV propagation by modulating the IFN-I via TBK1 protein [84], which was consistent with results showing duck DDX3X affecting DTMUV replication [101]. However, other quantitative proteomic analyses revealed that DDX5 was upregulated (1.84 fold) in DEF cells infected with DTMUV [102]; the result was different from DDX5 detection in mammalian cells, indicating that there might be a difference in the innate immune responses to DTMUV between avians and mammals.…”

Section: Other Prrs Relevant To Dtmuv Infectionmentioning

confidence: 99%

“…More importantly, the signaling proteins MyD88, TRAF3, and NF-κB were significantly upregulated [ 75 ], and DTMUV infection significantly increased the expression of IRF-1, indicating that the virus might induce MyD88 signaling pathway [ 83 ]. Differentially expressed proteins in DTMUV-infected BHK-21 cells were quantitatively identified by Sun et al by iTRAQ, the production of TLR9 increased at 48 hpi [ 84 ], which suggested that TLR9 can be potentially implicated in DTMUV infection in mammalian cell lines.…”

Section: Dtmuv Infection Triggers Host Innate Immune Responsesmentioning

confidence: 99%

Innate immune responses to duck Tembusu virus infection

Zhao

Yang

et al. 2020

Vet Res

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The disease caused by duck Tembusu virus (DTMUV) is characterized by severe egg-drop in laying ducks. Currently, the disease has spread to most duck-raising areas in China, leading to great economic losses in the duck industry. In the recent years, DTMUV has raised some concerns, because of its expanding host range and increasing pathogenicity, as well as the potential threat to public health. Innate immunity is crucial for defending against invading pathogens in the early stages of infection. Recently, studies on the interaction between DTMUV and host innate immune response have made great progress. In the review, we provide an overview of DTMUV and summarize current advances in our understanding of the interaction between DTMUV and innate immunity, including the host innate immune responses to DTMUV infection through pattern recognition receptors (PRRs), signaling transducer molecules, interferon-stimulated genes (ISGs), and the immune evasion strategies employed by DTMUV. The aim of the review is to gain an in-depth understanding of DTMUV pathogenesis to facilitate future studies.

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“…Isobaric tags for relative and absolute quantification (iTRAQ) as a high-throughput proteomics approach are useful for the analysis of infection-associated proteins of pathogens [22–24]. Sun et al [25] identified 192 significantly expressed host proteins in a DTMUV-infected baby hamster kidney cell line using the iTRAQ approach.…”

Section: Introductionmentioning

confidence: 99%

ITRAQ-Based Quantitative Proteomics Reveals the Proteome Profiles of Primary Duck Embryo Fibroblast Cells Infected with Duck Tembusu Virus

et al. 2019

BioMed Research International

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Outbreaks of duck Tembusu virus (DTMUV) have caused substantial economic losses in the major duck-producing regions of China since 2010. To improve our understanding of the host cellular responses to virus infection and the pathogenesis of DTMUV infection, we applied isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled with multidimensional liquid chromatography-tandem mass spectrometry to detect the protein changes in duck embryo fibroblast cells (DEFs) infected and mock-infected with DTMUV. In total, 434 cellular proteins were differentially expressed, among which 116, 76, and 339 proteins were differentially expressed in the DTMUV-infected DEFs at 12, 24, and 42 hours postinfection, respectively. The Gene Ontology analysis indicated that the biological processes of the differentially expressed proteins were primarily related to cellular processes, metabolic processes, biological regulation, response to stimulus, and cellular organismal processes and that the molecular functions in which the differentially expressed proteins were mainly involved were binding and catalytic activity. Some selected proteins that were found to be differentially expressed in DTMUV-infected DEFs were further confirmed by real-time PCR. The results of this study provide valuable insight into DTMUV-host interactions. This could lead to a better understanding of DTMUV infection mechanisms.

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Proteome analysis of Duck Tembusu virus (DTMUV)‐infected BHK‐21 cells

Cited by 15 publications

References 63 publications

Serological evidence of duck Tembusu virus infection in free-grazing ducks, Thailand

Serological evidence of duck Tembusu virus infection in free-grazing ducks, Thailand

Innate immune responses to duck Tembusu virus infection

ITRAQ-Based Quantitative Proteomics Reveals the Proteome Profiles of Primary Duck Embryo Fibroblast Cells Infected with Duck Tembusu Virus

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