Proteome analysis of infection-specific proteins from Japanese birch (Betula platyphylla var. japonica) plantlet No.8 infected with Inonotus obliquus strain IO-U1

Abstract

This study aimed to identify the proteins produced specifically in Betula platyphylla var. japonica plantlet No. 8 by infection with Inonotus obliquus strain IO-U1. Sterile Japanese birch plantlets were infected with the fungus, and the protein samples obtained at 2 days post-infection were subjected to two-dimensional electrophoresis to detect infectionspecific proteins. The specific proteins were analyzed using MALDI-TOF-MS and identified by peptide mass fingerprinting with MASCOT software. Among the 735 protein spots detected in the infected plantlets, 169 spots were recognized as infection-specific proteins. Of these spots, 91 were analyzed using MALDI-TOF-MS, with two heat shock proteins (Hsp70 and Hsp60) being identified as infection-specific proteins. Hsp70 and Hsp60 may cooperate to refold proteins that were denatured by the infection of I. obliquus strain IO-U1 in the birch plantlets. It is suggested that these proteins are expressed in B. platyphylla plantlet No. 8 by infectious stress with I. obliquus strain IO-U1.

Proteomic Analysis of Responsive Proteins Induced in Japanese Birch Plantlet Treated with Salicylic Acid

“…In our previous studies of pathogen resistance in Japanese birch plantlet No.8, heat shock 60 kDa and 70 kDa proteins were found to be specifically expressed during the infection of I. obliquus strain IO-U1 [ 29 ]. In addition, in anatomical and histochemical observations, lignification, phenolics deposition, and necrophylactic periderm formation have occurred as infection-induced responses in Japanese birch plantlet No.8 [ 30 ] and Tohoku [ 31 ] infected with I. obliquus strain IO-U1.…”