Proteome analysis of Lactobacillus plantarum strain under cheese-like conditions

Wu, Zhen; Wang, Pingping; He, Jianzhong; Pan, Daodong; Zeng, Xiaoqun; Cao, Jinxuan

doi:10.1016/j.jprot.2016.07.008

Cited by 12 publications

(6 citation statements)

References 25 publications

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“…The up-regulation of phosphoglycerate kinase ( pgk ), a crucial enzyme in glycolysis, accelerates the catalysis of the 1,3-bisphosphoglycerate to 3-phosphoglycerate, which serves as a precursor of lipoteichoic acid and mediate the characteristics of S-layer protein ( Cai et al, 2020 ). Glycogenesis and glycolysis are the main pathways for energy acquisition in bacteria cells, during which glucose is catalyzed to produce pyruvate and energy, and pyruvate can be further reduced to lactate ( Wu et al, 2016 ). It can be speculated that the enhanced cellular ATP and pyruvate synthesis supports higher survival rates of bacteria.…”

Section: Discussionmentioning

confidence: 99%

Effect and potential mechanism of nitrite reductase B on nitrite degradation by Limosilactobacillus fermentum RC4

Fan,

Xia,

Zeng

et al. 2024

Current Research in Food Science

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Section: Discussionmentioning

confidence: 99%

Effect and potential mechanism of nitrite reductase B on nitrite degradation by Limosilactobacillus fermentum RC4

Fan,

Xia,

Zeng

et al. 2024

Current Research in Food Science

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“…Aldehyde-alcohol dehydrogenase (adhE, D7VE84) was significantly upregulated in this study, perhaps promoting the production of ATP and NAD + during pyruvate metabolism when Salmonella sp. D194-2 was exposed to CIN (Wu et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of 1,8-cineole on Carbohydrate Metabolism Related Cell Structure Changes of Salmonella

et al. 2018

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Salmonella is gram-negative foodborne zoonotic bacteria which triggers disease in humans. Our previous studies showed 1,8-cineole possessed remarkable antimicrobial effects on foodborne zoonotic bacteria indicating it could serve as a new source of antibiotic for Salmonella. Present study elucidated the antibacterial mechanism of 1,8-cineole by analyzing serum protein expressed by Salmonella following treatment with 1,8-cineole (0.25 mg/mL, 3 h) using isobaric tags for relative and absolute quantification (iTRAQ) with two-dimensional liquid chromatography/tandem mass spectrometry (2D-LC-MS/MS). 1,8-cineole was found to significantly damage the structure of Salmonella cell walls and membranes. A total of 3011 proteins were extracted from the experimental group, of which 435 were differentially expressed (1.5-fold) with 123 upregulated and 312 downregulated. The expressed proteins were involved in 935 intracellular biological processes, 98 cellular components, 477 molecular functions and 86 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Among them, proteins associated with carbohydrate, nucleotide, amino acid, lipid, and energy metabolism were significantly changed following treatment with 1,8-cineole. Carbohydrate metabolism and membrane protein-related genes was down-regulated at the mRNA level when Salmonella was treated with 1,8-cineole. 1,8-cineole may be a potential antibiotic for Salmonella infections.

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“…The high specificity could be ascribed to the fact that only L. casei cells can be simultaneously recognized by the 2 aptamers and resulted in fluorescent signals for detection. For L. plantarum, fluorescent intensity higher than that of other 4 reference bacteria was observed, a phenomenon that was probably caused by the partially shared membrane protein motif between L. casei and L. plantarum (Shao et al, 2015;Wu et al, 2016). However, this fluorescent intensity was particularly low in comparison to that rendered by L. casei cells, ensuring the detection specificity of the established strategy.…”

Section: Analysis Of L Casei Cells In Viable and Nonviable Conditionmentioning

confidence: 90%

Rapid identification and quantitation of the viable cells of Lactobacillus casei in fermented dairy products using an aptamer-based strategy powered by a novel cell-SELEX protocol

Song

Wang

et al. 2019

Journal of Dairy Science

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An aptamer-based strategy was developed for qualitative and quantitative analysis of viable Lactobacillus casei in dairy products. Three highly specific aptamers for L. casei were obtained using systematic evolution of ligands by exponential enrichment protocol using the whole bacterium cell as the target (cell-SELEX) facilitated by polyethyleneglycol and chitosan modified graphene oxide and complementary ring-mediated rolling circle amplification. Two aptamers, one for separating and enriching the L. casei cells and the other for generating fluorescence signals, were employed to develop an aptamer-based strategy, which was demonstrated for the selective detection of L. casei in commercial dairy drinks, with a dynamic range of 10 5 to 10 9 cfu/mL. Viable and nonviable L. casei cells could be discriminated based on the significant difference in fluorescence intensity. This established strategy is of high selectivity and sensitivity, and can be used for rapid analysis of viable L. casei in quality control and food surveillance areas.

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Proteome analysis of Lactobacillus plantarum strain under cheese-like conditions

Cited by 12 publications

References 25 publications

Effect and potential mechanism of nitrite reductase B on nitrite degradation by Limosilactobacillus fermentum RC4

Effect and potential mechanism of nitrite reductase B on nitrite degradation by Limosilactobacillus fermentum RC4

Effects of 1,8-cineole on Carbohydrate Metabolism Related Cell Structure Changes of Salmonella

Rapid identification and quantitation of the viable cells of Lactobacillus casei in fermented dairy products using an aptamer-based strategy powered by a novel cell-SELEX protocol

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