Proteome analysis of leaves from the resurrection plant Boea hygrometrica in response to dehydration and rehydration

Abstract

Resurrection plants differ from other species in their unique ability to survive desiccation. In order to understand the mechanisms of desiccation tolerance, proteome studies were carried out using leaves of the resurrection plant Boea hygrometrica to reveal proteins that were differentially expressed in response to changes in relative water content. This opportunity was afforded by the rare ability of excised B. hygrometrica leaves to survive and resume metabolism following desiccation in a manner similar to intact plants. From a total of 223 proteins that were reproducibly detected and analyzed, 35% showed increased abundance in dehydrated leaves, 5% were induced in rehydrated leaves and 60% showed decreased or unchanged abundance in dehydrated and rehydrated leaves. Since the induction kinetics fall into clearly defined patterns, we suggest that programmed regulation of protein expression triggered by changes of water status. Fourteen dehydration responsive proteins were analyzed by mass spectrometry. Eight proteins were classified as playing a role in reactive oxygen species scavenging, photosynthesis and energy metabolism. In agreement with these findings, glutathione content and polyphenol oxidase activity were found to increase upon dehydration and rapid recovery of photosynthesis was observed.

Photoprotection Conferred by Changes in Photosynthetic Protein Levels and Organization during Dehydration of a Homoiochlorophyllous Resurrection Plant

“…Elevated levels of the thylakoid protease FtsH have been observed at late stages of dehydration of the poikilochlorophyllous resurrection plant Xerophyta viscosa (Ingle et al, 2007), in which degradation of thylakoid proteins is a natural occurrence during desiccation. However, the observation of a similar phenomenon in homoiochlorophyllous types such as Selaginella tamariscina (Wang et al, 2010), H. rhodopensis (Georgieva et al, 2009;Sárvári et al, 2014), and Boea hygrometrica (Jiang et al, 2007) suggests . The density of PSII complexes in grana thylakoid membranes changes during dehydration and rehydration.…”

Proteome Analysis of Detached Fronds from a Resurrection Plant Selaginella Bryopteris - Response to Dehydration and Rehydration

“…This was ascertained by studying various physiological parameters in intact plants as well as in detached fronds (data not shown). The desiccation and rehydration of detached fronds avoid interference from developmental regulation and long-distance signalling from other organs (Jiang et al, 2007). We intend to use it as a model system to gain a system-level understanding of responses to desiccation using multiple platforms that provide information about global transcript levels, proteomes, a wide range of metabolites, enzyme activities, and growth parameters.…”

A Sister Group Contrast Using Untargeted Global Metabolomic Analysis Delineates the Biochemical Regulation Underlying Desiccation Tolerance in Sporobolus stapfianus

“…Antioxidants and their attending enzymes also appear central to the desiccation response in DT plants and tissues (Sherwin and Farrant, 1998;Illing et al, 2005;Kranner and Birtic, 2005;Berjak, 2006;Farrant, 2007). For example, ascorbate-glutathione cycle metabolites are often elevated during drying to combat ROS activity in resurrection plants Jiang et al, 2007). In fact, the length of time a resurrection plant can survive the dried state has been correlated to the level of antioxidants in its tissue (Kranner et al, 2002).…”